##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546878_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1230833
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.660408845066716	31.0	31.0	34.0	30.0	34.0
2	31.999384969366275	31.0	31.0	34.0	30.0	34.0
3	31.95172293885523	31.0	31.0	34.0	30.0	34.0
4	35.763983416109255	37.0	35.0	37.0	35.0	37.0
5	35.77240372983175	37.0	35.0	37.0	35.0	37.0
6	35.81616596240107	37.0	35.0	37.0	35.0	37.0
7	36.014460938242635	37.0	35.0	37.0	35.0	37.0
8	13.293542665820627	0.0	0.0	35.0	0.0	37.0
9	24.893188596665834	18.0	17.0	38.0	17.0	39.0
10	32.54539730410218	32.0	32.0	37.0	27.0	39.0
11	35.487118886152714	37.0	34.0	37.0	32.0	39.0
12	36.631714456794704	37.0	35.0	39.0	33.0	39.0
13	36.91168907560977	39.0	37.0	39.0	33.0	39.0
14	38.13260856671864	40.0	37.0	41.0	33.0	41.0
15	38.34923096797047	40.0	37.0	41.0	34.0	41.0
16	38.42022516458366	40.0	38.0	41.0	34.0	41.0
17	38.35694931806346	40.0	37.0	41.0	34.0	41.0
18	38.28327563528115	40.0	37.0	41.0	33.0	41.0
19	38.23886506130401	40.0	37.0	41.0	34.0	41.0
20	38.15353991971291	40.0	37.0	41.0	34.0	41.0
21	38.12346922775064	40.0	37.0	41.0	33.0	41.0
22	38.06012838459807	40.0	36.0	41.0	33.0	41.0
23	37.89507837375176	39.0	36.0	41.0	33.0	41.0
24	37.69400722925043	40.0	35.0	41.0	33.0	41.0
25	37.597206932215826	39.0	35.0	41.0	33.0	41.0
26	37.50899756506366	39.0	35.0	41.0	33.0	41.0
27	37.44133363340112	39.0	35.0	41.0	33.0	41.0
28	37.31560171038638	39.0	35.0	41.0	33.0	41.0
29	37.2706272906235	39.0	35.0	41.0	33.0	41.0
30	37.13138744248813	39.0	35.0	41.0	32.0	41.0
31	37.01209343590885	39.0	35.0	41.0	32.0	41.0
32	36.74587129204368	39.0	35.0	41.0	31.0	41.0
33	36.47121421021373	39.0	35.0	41.0	30.0	41.0
34	36.14562657972284	39.0	35.0	41.0	29.0	41.0
35	35.908402683385965	39.0	35.0	41.0	27.0	41.0
36	35.75156499703859	39.0	35.0	41.0	26.0	41.0
37	35.677281970827885	39.0	35.0	41.0	26.0	41.0
38	35.56683481837097	39.0	35.0	41.0	25.0	41.0
39	35.46085699684685	39.0	35.0	41.0	24.0	41.0
40	35.356169358475114	39.0	35.0	40.0	24.0	41.0
41	35.26706385025426	39.0	35.0	40.0	24.0	41.0
42	35.190689557397306	39.0	35.0	40.0	23.0	41.0
43	35.07249968111027	38.0	35.0	40.0	23.0	41.0
44	34.89488988351791	38.0	34.0	40.0	23.0	41.0
45	34.82526142864223	38.0	34.0	40.0	23.0	41.0
46	34.787391140796515	38.0	34.0	40.0	23.0	41.0
47	34.71024013818284	38.0	34.0	40.0	23.0	41.0
48	34.627054198254356	38.0	34.0	40.0	23.0	41.0
49	34.52083101444306	38.0	34.0	40.0	23.0	41.0
50	34.410133624951555	37.0	34.0	40.0	22.0	41.0
51	34.053038064465284	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	5.0
12	1.0
13	7.0
14	12.0
15	24.0
16	55.0
17	177.0
18	430.0
19	938.0
20	1781.0
21	3123.0
22	5148.0
23	8102.0
24	12438.0
25	18210.0
26	23108.0
27	24379.0
28	23350.0
29	23370.0
30	26644.0
31	32628.0
32	42288.0
33	57642.0
34	84824.0
35	125395.0
36	133777.0
37	214398.0
38	257935.0
39	110626.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.9162721506492	20.504162628073832	25.17652679120563	19.403038430071344
2	37.386550409356914	23.683879129012627	26.603446608922575	12.326123852707882
3	28.432045614636593	24.46156383522379	34.342189395311955	12.764201154827665
4	25.182213996537307	25.099749519228038	34.588607877754335	15.129428606480328
5	22.8122742890384	29.267739815230826	32.782676447576556	15.137309448154218
6	20.24645098075856	38.14051134475595	31.414253598985404	10.198784075500088
7	79.1121135036191	4.699175273981117	13.744106633475054	2.444604588924736
8	29.620590283165953	63.9607485337166	4.914801601842004	1.5038595812754452
9	73.94301257766081	5.346216749144685	15.18548820189254	5.5252824713019555
10	40.780105830766644	23.00962031404748	21.370648983249556	14.839624871936321
11	34.21243986795935	22.21593018711718	27.383162459895043	16.188467485028433
12	30.223352802532922	20.390337275649905	31.98565524323771	17.400654678579468
13	23.080791626483855	25.754834327646396	33.31938613930566	17.844987906564093
14	18.82001863778433	29.949473242917602	34.05352310183429	17.17698501746378
15	17.91924655903766	22.441630992994174	41.87968635875054	17.75943608921763
16	20.461346096505377	20.632530976988754	39.43240065874087	19.473722267765
17	20.554291280783016	20.7388004709006	32.70492422611353	26.00198402220285
18	22.614197051915248	22.619234290923302	34.05368559341519	20.71288306374626
19	25.390203220095657	24.344488651181763	30.029500346513295	20.235807782209285
20	27.100670846491763	23.681847984251316	28.834456014747733	20.383025154509184
21	24.159248248950103	25.714780152953328	31.496068109971056	18.62990348812552
22	22.906844389125087	21.68628887915745	31.848106120001656	23.558760611715808
23	22.190175271543744	25.5110157104985	30.280874822173274	22.01793419578448
24	22.09292406037212	23.94784670219274	34.200090507810565	19.75913872962457
25	21.23829959060246	24.04201057332717	32.75895267676444	21.960737159305936
26	19.183024829526023	25.961442372767063	31.785790598724606	23.069742198982315
27	21.235780971098436	24.766723024163312	31.89725982322541	22.100236181512845
28	19.6176898084468	25.16767100004631	35.371248577183096	19.843390614323795
29	20.905273095537737	23.722145896315748	35.19860127247157	20.173979735674948
30	21.932707361599828	24.572789322353238	33.63908832473618	19.855414991310763
31	25.89855813095684	23.604989466483268	29.49547176586913	21.000980636690763
32	26.281875770311654	23.950771550649034	30.634943976965197	19.132408702074123
33	25.460643320417958	23.88658737619157	29.563230755106503	21.08953854828397
34	21.049321882009988	25.90993254162019	32.03667759964187	21.004067976727956
35	21.867791975028293	25.35851736181919	31.019480303176795	21.754210359975723
36	24.915890295434068	26.300562302115722	28.576906859013366	20.206640543436844
37	22.550337860619596	27.71643269233113	29.09379257787206	20.639436869177217
38	22.066194195313255	28.97631116487777	26.54763075088172	22.409863888927255
39	21.87542907933083	27.21498367365841	27.14129374171801	23.768293505292757
40	22.426519275970016	24.51372363269428	28.77896513986869	24.280791951467016
41	20.61303198728016	24.723419017852137	28.585681404382235	26.07786759048547
42	22.19033776312465	26.28577556825337	27.436134715270065	24.087751953351916
43	22.172219951853744	25.6796007256874	27.593995286119238	24.554184036339617
44	21.99266675495376	26.856121017229796	28.97241136693605	22.178800860880397
45	21.12398676343582	27.82383962730931	28.039384709379746	23.012788899875126
46	22.788631764016724	27.255606568884648	28.93349463330931	21.022267033789312
47	21.936038439008378	25.94844304629466	29.64528900346351	22.47022951123345
48	22.567561968195523	25.173276959587533	29.660644457859025	22.59851661435792
49	22.20244338590207	24.19580885465372	30.96309572460277	22.638652034841446
50	20.5750089573484	26.171868970038993	30.665898623127593	22.587223449485023
51	21.008049020460128	27.207671552517688	28.994835205100937	22.78944422192125
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1119.0
1	1889.5
2	2660.0
3	17984.5
4	33309.0
5	22311.0
6	11313.0
7	11130.0
8	10947.0
9	10932.5
10	10918.0
11	10779.0
12	10640.0
13	10209.5
14	9779.0
15	9214.5
16	8650.0
17	8076.0
18	7502.0
19	7160.5
20	6819.0
21	6759.5
22	6700.0
23	7390.5
24	8081.0
25	8338.5
26	9534.5
27	10473.0
28	11890.0
29	13307.0
30	15313.0
31	17319.0
32	19247.0
33	21175.0
34	24106.5
35	27038.0
36	30003.0
37	32968.0
38	40173.0
39	47378.0
40	57156.0
41	66934.0
42	75125.0
43	83316.0
44	90758.5
45	98201.0
46	95782.5
47	93364.0
48	90113.5
49	86863.0
50	83911.0
51	80959.0
52	76617.0
53	72275.0
54	70100.5
55	67926.0
56	65664.5
57	63403.0
58	59278.5
59	55154.0
60	50314.0
61	45474.0
62	40509.0
63	35544.0
64	30276.5
65	25009.0
66	21138.5
67	17268.0
68	14729.5
69	12191.0
70	10373.0
71	8555.0
72	7071.0
73	5587.0
74	4344.0
75	2347.0
76	1593.0
77	1184.5
78	776.0
79	562.5
80	349.0
81	272.0
82	195.0
83	131.0
84	67.0
85	42.5
86	18.0
87	16.0
88	14.0
89	8.5
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1230833.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.10044542478035
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.828389065943	22.736228377364124
2	7.01650198918705	4.504656783535233
3	3.7132958374597638	3.575953511293236
4	2.6002384277262545	3.338752469625731
5	1.9998733917449214	3.209841333408911
6	1.7613221592187436	3.392353550847453
7	1.4401368198780362	3.2360323373498137
8	1.294032291349732	3.323121035710043
9	1.1536963024137727	3.333074867316377
>10	8.105043028455798	43.829154285907066
>50	0.0593417395945915	1.2053789562873547
>100	0.025316052393530866	1.6968104542404254
>500	0.001022870776292435	0.22787217626498743
>1k	0.0012785884703655438	0.6875566816986798
>5k	2.5571769407310875E-4	0.6023508751557918
>10k+	2.5571769407310875E-4	1.1008623039948655
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13411	1.0895872957582386	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7338	0.5961816103403142	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCG	2334	0.18962767491609342	No Hit
GAATCTGACTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC	2284	0.1855653853934693	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC	1481	0.12032501566012611	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1232	0.10009481383745804	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.011780639615609916	0.0
2	0.0	0.0	0.0	0.055572120669497814	0.0
3	0.0	0.0	0.0	0.09635750747664387	0.0
4	0.0	0.0	0.0	0.17362225419695443	0.0
5	0.0	0.0	0.0	0.39006103996236696	0.0
6	0.0	0.0	0.0	0.5870820818096363	0.0
7	0.0	0.0	0.0	0.7226000602843765	0.0
8	0.0	0.0	0.0	0.9918486098439024	0.0
9	0.0	0.0	0.0	1.3925528483555445	0.0
10	0.0	0.0	0.0	2.1406640868420004	0.0
11	0.0	0.0	0.0	2.4524854305986272	0.0
12	0.0	0.0	0.0	2.817279029730272	0.0
13	0.0	0.0	0.0	2.970427344733201	0.0
14	0.0	0.0	0.0	3.0476108456630593	0.0
15	0.0	0.0	0.0	3.1250380839642746	0.0
16	0.0	0.0	0.0	3.232607510523361	0.0
17	0.0	0.0	0.0	3.3436705060719043	0.0
18	0.0	0.0	0.0	3.5412602684523407	0.0
19	0.0	0.0	0.0	3.645742354974233	0.0
20	0.0	0.0	0.0	3.7408811755940894	0.0
21	0.0	0.0	0.0	3.8404072688983804	0.0
22	0.0	0.0	0.0	3.95626376608362	0.0
23	0.0	0.0	0.0	4.0725264922211215	0.0
24	0.0	0.0	0.0	4.1666091175650966	0.0
25	0.0	0.0	0.0	4.249073594874366	0.0
26	0.0	0.0	0.0	4.339093930695716	0.0
27	0.0	0.0	0.0	4.437320091352767	0.0
28	0.0	0.0	0.0	4.535465006219365	0.0
29	0.0	0.0	0.0	4.631822513696009	0.0
30	0.0	0.0	0.0	4.771565273274279	0.0
31	0.0	0.0	0.0	4.881003353013772	0.0
32	0.0	0.0	0.0	5.0081530150719065	0.0
33	0.0	0.0	0.0	5.130590421283797	0.0
34	0.0	0.0	0.0	5.2441720363363675	0.0
35	0.0	0.0	0.0	5.370346748909072	0.0
36	0.0	0.0	0.0	5.4996088015189715	0.0
37	0.0	0.0	0.0	5.624727318815794	0.0
38	0.0	0.0	0.0	5.784375297054921	0.0
39	0.0	0.0	0.0	5.960353679174998	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGATC	20	7.0334825E-4	45.0	11
CTACCGT	20	7.0334825E-4	45.0	29
TAATGCG	20	7.0334825E-4	45.0	1
CGTTTTT	10435	0.0	43.33972	1
TGTACGA	110	0.0	42.954548	2
GTTTTTA	6965	0.0	42.286434	2
CGGTCTA	220	0.0	41.93182	31
TCGATAG	55	6.184564E-11	40.909092	1
GAGCGAT	1015	0.0	40.788177	7
TATACGG	50	1.0822987E-9	40.500004	2
CGTTAGA	85	0.0	39.705883	2
CGAGAAT	120	0.0	39.375004	6
TATTTTT	8025	0.0	38.887848	7
AGGGGAA	6460	0.0	38.86997	8
AGCGATC	180	0.0	38.75	8
CGACGGT	250	0.0	38.7	28
ATAACGC	70	0.0	38.571426	11
TAATCGG	35	6.2486506E-6	38.571426	2
TTTTATT	8120	0.0	38.46059	4
TTATTTT	8110	0.0	38.45253	6
>>END_MODULE