FastQCFastQC Report
Thu 26 May 2016
SRR1546877_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546877_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences875067
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143581.6407886481835106No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79610.9097589098891856No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC15920.18192892658505005No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAACC207.032364E-445.09
ACCGCTA207.032364E-445.010
CCAATCG1000.045.024
GTCTACG207.032364E-445.01
TTACGTC207.032364E-445.024
GAACCGT207.032364E-445.07
TAATGCG406.8121153E-945.01
CCGCAAT502.1827873E-1145.033
CTAGCGA253.8900405E-545.02
GCCGGAT302.1649066E-644.99999619
CGCCGGA302.1649066E-644.99999618
TCGACGA302.1649066E-644.9999962
TGGTACG302.1649066E-644.9999961
TATCCGA302.1649066E-644.9999962
CGTTTTT95600.044.4116131
GTTTTTA62900.043.962642
CACGACG4100.043.9024426
ACGGTCT4100.043.9024430
CGCATCG1750.043.71428721
ATTGCGA1100.042.9545442
Produced by FastQC (version 0.11.5)