##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546877_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	875067
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.714115604862258	31.0	31.0	34.0	30.0	34.0
2	32.04864541800799	33.0	31.0	34.0	30.0	34.0
3	31.942949511294565	33.0	31.0	34.0	30.0	34.0
4	35.76869314006813	37.0	35.0	37.0	35.0	37.0
5	35.79370951024322	37.0	35.0	37.0	35.0	37.0
6	35.86123348269332	37.0	35.0	37.0	35.0	37.0
7	36.10217617622422	37.0	35.0	37.0	35.0	37.0
8	13.234194638810514	0.0	0.0	36.0	0.0	37.0
9	24.925365714853832	18.0	17.0	39.0	17.0	39.0
10	32.59675316290067	32.0	32.0	37.0	27.0	39.0
11	35.57003178042367	37.0	35.0	37.0	32.0	39.0
12	36.704171223460605	38.0	35.0	39.0	33.0	39.0
13	36.93454558336676	39.0	37.0	39.0	33.0	39.0
14	38.19283894833195	40.0	37.0	41.0	33.0	41.0
15	38.45536398927168	40.0	38.0	41.0	34.0	41.0
16	38.52884750539101	40.0	38.0	41.0	34.0	41.0
17	38.5201555995141	40.0	38.0	41.0	34.0	41.0
18	38.44350089764555	40.0	37.0	41.0	34.0	41.0
19	38.37599978058823	40.0	37.0	41.0	34.0	41.0
20	38.29285871824672	40.0	37.0	41.0	34.0	41.0
21	38.238182904851854	40.0	37.0	41.0	34.0	41.0
22	38.17843205148863	40.0	36.0	41.0	34.0	41.0
23	37.96985145137458	39.0	36.0	41.0	34.0	41.0
24	37.66477081183498	39.0	35.0	41.0	33.0	41.0
25	37.53222667521458	39.0	35.0	41.0	33.0	41.0
26	37.431776081145784	39.0	35.0	41.0	33.0	41.0
27	37.35710751291044	39.0	35.0	41.0	33.0	41.0
28	37.25574041759088	39.0	35.0	41.0	33.0	41.0
29	37.171527437327654	39.0	35.0	41.0	33.0	41.0
30	37.01392350528588	39.0	35.0	41.0	33.0	41.0
31	36.82775604610847	39.0	35.0	41.0	32.0	41.0
32	36.49282512081932	39.0	35.0	41.0	31.0	41.0
33	36.143574149179436	39.0	35.0	41.0	30.0	41.0
34	35.75220868802046	39.0	35.0	41.0	27.0	41.0
35	35.46108697962556	39.0	35.0	41.0	24.0	41.0
36	35.25836764499176	39.0	35.0	41.0	23.0	41.0
37	35.16260240644431	39.0	35.0	41.0	23.0	41.0
38	35.03291633669193	38.0	35.0	40.0	23.0	41.0
39	34.97306606237008	38.0	35.0	40.0	22.0	41.0
40	34.853350657721066	38.0	35.0	40.0	22.0	41.0
41	34.77755646139096	38.0	35.0	40.0	21.0	41.0
42	34.71215346939149	38.0	35.0	40.0	21.0	41.0
43	34.59774394417799	38.0	34.0	40.0	20.0	41.0
44	34.389566741746634	38.0	34.0	40.0	18.0	41.0
45	34.27335963989043	38.0	34.0	40.0	20.0	41.0
46	34.2190620832462	38.0	34.0	40.0	19.0	41.0
47	34.1712006052108	38.0	34.0	40.0	18.0	41.0
48	34.0923894970328	38.0	34.0	40.0	18.0	41.0
49	33.99433529089772	37.0	34.0	40.0	18.0	41.0
50	33.85930677308138	37.0	33.0	40.0	18.0	41.0
51	33.52235085999129	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	3.0
14	7.0
15	13.0
16	26.0
17	144.0
18	275.0
19	671.0
20	1345.0
21	2383.0
22	3939.0
23	6551.0
24	10830.0
25	16475.0
26	19631.0
27	18964.0
28	16826.0
29	16465.0
30	18026.0
31	22281.0
32	29081.0
33	40279.0
34	60524.0
35	100789.0
36	89979.0
37	143817.0
38	182304.0
39	73413.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.365484014366903	21.859469046370165	26.216849681224407	20.558197258038525
2	38.70549340793333	23.863772716831967	26.350210898136943	11.080522977097754
3	27.33196429530539	23.67007326296158	36.95157056545384	12.046391876279188
4	24.129352381017682	24.75204755750131	36.800839250023145	14.317760811457866
5	20.86045982764748	29.621731821677656	36.10294983127007	13.414858519404799
6	18.36807924421787	38.37866129107828	33.482121940377134	9.77113752432671
7	79.46294398028951	4.335782288670467	14.561742129459802	1.6395316015802217
8	30.066497765313972	63.72094936730559	5.280738503451736	0.931814363928705
9	76.03497789312132	5.2318279628874125	15.741423228164244	2.9917709158270167
10	40.07750263694094	23.79200678348058	23.045549655054984	13.084940924523494
11	35.22381714771555	23.015380536576057	27.042157914765387	14.718644400943012
12	31.54649872524047	22.09785079313927	31.812192666390114	14.543457815230148
13	21.438015603376655	33.48040778591811	31.59369511134576	13.487881499359478
14	16.69986412468988	37.51164196570092	32.21753305746874	13.570960852140464
15	14.376042063064887	25.76191308779785	44.961471521609205	14.900573327528063
16	15.933065696683796	21.22271780332249	44.98729811545859	17.856918384535128
17	15.636402698307672	21.4291020001897	34.653575097678235	28.280920203824394
18	19.350404026205993	25.43473814005099	36.37744309864273	18.837414735100282
19	24.246600546015333	27.96654427603829	29.669499592602623	18.11735558534375
20	25.168015706225923	25.71174550063024	29.20382096456614	19.9164178285777
21	21.125582383977456	27.941289067008583	32.738521736049925	18.194606812964036
22	20.55305479466144	24.721878439022383	30.695135343922235	24.029931422393943
23	17.496146009391282	31.065506984036652	30.08912460417317	21.3492224023989
24	18.754335382319294	28.674147236725872	36.75558557230475	15.81593180865008
25	18.642801065518412	27.073926910739406	35.61144460938419	18.671827414357985
26	16.65221063072885	31.724999342907456	31.36811238453741	20.254677641826284
27	17.957367835834283	29.844571901351554	33.570343756535216	18.62771650627895
28	15.818788732748464	28.301032949477012	38.7739453093306	17.106233008443926
29	16.02631569925503	27.55400443623174	37.76167996279142	18.657999901721812
30	18.711938628699286	28.600552871951518	35.59521728050537	17.092291218843812
31	25.597239982767032	26.999418330253572	29.113656440021163	18.289685246958232
32	24.112439390355252	28.090191951016326	30.641768001764436	17.15560065686399
33	22.801111229197307	27.34899156293175	28.913443199206462	20.93645400866448
34	18.382706695601595	28.881445649304567	31.348685300668404	21.38716235442543
35	19.634153727657424	26.76846458614026	32.07434402165777	21.523037664544546
36	25.57335609730455	26.874513608672252	29.619217728471074	17.932912565552122
37	20.26530539947227	30.962886270422725	29.71532465514069	19.056483674964316
38	19.622840308227826	33.33310477940547	26.874627885636187	20.169427026730524
39	20.034008824467158	30.363617871545834	27.80644224956489	21.79593105442212
40	21.322710146765907	27.159977464582713	29.37603634921669	22.141276039434697
41	18.73547968326997	25.82156566297209	30.292309046050185	25.15064560770775
42	20.6994435854626	30.22465708340047	26.567337129614078	22.508562201522857
43	21.004334525242065	29.437288801886023	27.119180588457798	22.439196084414107
44	20.247592470062294	31.834476674357504	28.939269793055843	18.978661062524356
45	19.090081102361307	34.27806099418673	26.242904829001667	20.3889530744503
46	22.75277207345266	31.653804794375745	27.77364476091545	17.81977837125614
47	21.215746908522434	30.379159538641044	27.907234531755854	20.49785902108067
48	21.450014684589867	29.193078929956222	28.96521066386917	20.391695721584746
49	20.16931274976659	27.509779251188764	30.349447528017855	21.97146047102679
50	19.347661379071546	30.416299551920023	30.082839371156723	20.153199697851708
51	19.194873078290005	32.57727693993717	27.705992798265733	20.52185718350709
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	953.0
1	1193.0
2	1433.0
3	15127.0
4	28821.0
5	18817.0
6	8813.0
7	9680.0
8	10547.0
9	11601.0
10	12655.0
11	13074.0
12	13493.0
13	13047.0
14	12601.0
15	11745.5
16	10890.0
17	10049.0
18	9208.0
19	8650.5
20	8093.0
21	7685.5
22	7278.0
23	7065.0
24	6852.0
25	6929.5
26	7646.0
27	8285.0
28	9479.5
29	10674.0
30	12116.5
31	13559.0
32	15118.5
33	16678.0
34	18819.5
35	20961.0
36	23989.5
37	27018.0
38	33986.5
39	40955.0
40	49721.5
41	58488.0
42	63394.5
43	68301.0
44	70559.5
45	72818.0
46	74788.5
47	76759.0
48	73839.5
49	70920.0
50	65529.0
51	60138.0
52	53726.0
53	47314.0
54	42254.0
55	37194.0
56	33352.5
57	29511.0
58	25941.0
59	22371.0
60	19009.5
61	15648.0
62	13639.0
63	11630.0
64	9821.0
65	8012.0
66	6716.5
67	5421.0
68	5033.0
69	4645.0
70	4404.5
71	4164.0
72	3416.5
73	2669.0
74	1962.5
75	911.5
76	567.0
77	390.0
78	213.0
79	183.5
80	154.0
81	112.0
82	70.0
83	42.0
84	14.0
85	11.5
86	9.0
87	6.0
88	3.0
89	2.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	875067.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.99007964280275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.63620090856229	27.974451371097054
2	8.820614337518398	6.701916823615435
3	4.116325703828705	4.691386239725057
4	2.5171040330282826	3.8249993073585786
5	1.7386499381923302	3.3025724811440353
6	1.3149706237756416	2.9973503235109584
7	1.0434531099989404	2.7748606726672977
8	0.8506256056397843	2.5852267603570183
9	0.6840426353765409	2.3388150777324723
>10	5.154191766345887	35.164466766085376
>50	0.09090309349499645	2.2070866774838014
>100	0.030804228553180876	2.403267989925903
>500	0.001208008962855427	0.29026915617871485
>1k	3.020022407138567E-4	0.18265157969822693
>5k	3.020022407138567E-4	0.9133726293829048
>10k+	3.020022407138567E-4	1.6473061440371497
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14358	1.6407886481835106	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7961	0.9097589098891856	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1592	0.18192892658505005	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007885110511537974	0.0
2	0.0	0.0	0.0	0.0409111530888492	0.0
3	0.0	0.0	0.0	0.08193658314163373	0.0
4	0.0	0.0	0.0	0.11850521160094027	0.0
5	0.0	0.0	0.0	0.21655484665745595	0.0
6	0.0	0.0	0.0	0.37962807419317607	0.0
7	0.0	0.0	0.0	0.49401931509244434	0.0
8	0.0	0.0	0.0	0.7112598235335122	0.0
9	0.0	0.0	0.0	1.0958018071759077	0.0
10	0.0	0.0	0.0	1.6596443472328404	0.0
11	0.0	0.0	0.0	1.990019049969888	0.0
12	0.0	0.0	0.0	2.3011952227657995	0.0
13	0.0	0.0	0.0	2.466325435652356	0.0
14	0.0	0.0	0.0	2.5530616512792736	0.0
15	0.0	0.0	0.0	2.628141616584787	0.0
16	0.0	0.0	0.0	2.735790516611871	0.0
17	0.0	0.0	0.0	2.851895911970169	0.0
18	0.0	0.0	0.0	3.077707192706387	0.0
19	0.0	0.0	0.0	3.1650147931529813	0.0
20	0.0	0.0	0.0	3.2720923083603886	0.0
21	0.0	0.0	0.0	3.3782556078563126	0.0
22	0.0	0.0	0.0	3.4841903534243666	0.0
23	0.0	0.0	0.0	3.6245224651369554	0.0
24	0.0	0.0	0.0	3.726686070895143	0.0
25	0.0	0.0	0.0	3.810108254567936	0.0
26	0.0	0.0	0.0	3.898330070726013	0.0
27	0.0	0.0	0.0	3.993065673828404	0.0
28	0.0	0.0	0.0	4.08597284550783	0.0
29	0.0	0.0	0.0	4.198307101056262	0.0
30	0.0	0.0	0.0	4.352923833260768	0.0
31	0.0	0.0	0.0	4.465600919701005	0.0
32	1.1427696393533295E-4	0.0	0.0	4.592791180561031	0.0
33	1.1427696393533295E-4	0.0	0.0	4.714267593224291	0.0
34	2.285539278706659E-4	0.0	0.0	4.838029545166256	0.0
35	2.285539278706659E-4	0.0	0.0	4.984075505075611	0.0
36	2.285539278706659E-4	0.0	0.0	5.107608903089706	0.0
37	2.285539278706659E-4	0.0	0.0	5.231942239851349	0.0
38	2.285539278706659E-4	0.0	0.0	5.35410431429822	0.0
39	2.285539278706659E-4	0.0	0.0	5.48997962441733	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAACC	20	7.032364E-4	45.0	9
ACCGCTA	20	7.032364E-4	45.0	10
CCAATCG	100	0.0	45.0	24
GTCTACG	20	7.032364E-4	45.0	1
TTACGTC	20	7.032364E-4	45.0	24
GAACCGT	20	7.032364E-4	45.0	7
TAATGCG	40	6.8121153E-9	45.0	1
CCGCAAT	50	2.1827873E-11	45.0	33
CTAGCGA	25	3.8900405E-5	45.0	2
GCCGGAT	30	2.1649066E-6	44.999996	19
CGCCGGA	30	2.1649066E-6	44.999996	18
TCGACGA	30	2.1649066E-6	44.999996	2
TGGTACG	30	2.1649066E-6	44.999996	1
TATCCGA	30	2.1649066E-6	44.999996	2
CGTTTTT	9560	0.0	44.411613	1
GTTTTTA	6290	0.0	43.96264	2
CACGACG	410	0.0	43.90244	26
ACGGTCT	410	0.0	43.90244	30
CGCATCG	175	0.0	43.714287	21
ATTGCGA	110	0.0	42.954544	2
>>END_MODULE