FastQCFastQC Report
Thu 26 May 2016
SRR1546876_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546876_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1194561
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129451.0836616966400208No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72950.6106845945916534No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG207.0333976E-445.01
CGTTTTT111400.043.545781
TTTAGCG1150.043.043481
GTTTTTA76650.042.2994162
TATACGA556.184564E-1140.9090922
CGACGGT1950.040.38461728
ATAACGC950.040.26315711
CGGTCTA2000.039.37500431
TATGCGA800.039.3752
GCGTAAG403.4583718E-739.3751
GAGCGAT9700.038.969077
TATTTTT86800.038.752887
TCGTTTA700.038.57142638
GCCGACC700.038.57142614
TTATTTT88000.038.4545446
CGTAGGA659.094947E-1238.076922
TTTTATT89600.037.9436384
AGGGGAA66750.037.550568
CTCACGA2100.037.524
TACGCAA301.139949E-437.4999961