##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546876_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1194561
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.741271479648173	31.0	31.0	34.0	30.0	34.0
2	32.09404375331189	33.0	31.0	34.0	30.0	34.0
3	32.043806050925824	33.0	31.0	34.0	30.0	34.0
4	35.846862571270954	37.0	35.0	37.0	35.0	37.0
5	35.86285087157542	37.0	35.0	37.0	35.0	37.0
6	35.90728476821192	37.0	35.0	37.0	35.0	37.0
7	36.1015787389677	37.0	35.0	37.0	35.0	37.0
8	13.201839001942973	0.0	0.0	35.0	0.0	37.0
9	24.877137291440118	18.0	17.0	38.0	17.0	39.0
10	32.66951038917226	32.0	32.0	38.0	27.0	39.0
11	35.6166700570335	37.0	35.0	37.0	32.0	39.0
12	36.739881847808526	38.0	35.0	39.0	33.0	39.0
13	37.016183351038585	39.0	37.0	39.0	33.0	39.0
14	38.24100234312019	40.0	37.0	41.0	34.0	41.0
15	38.48704419447814	40.0	38.0	41.0	34.0	41.0
16	38.53568968014191	40.0	38.0	41.0	34.0	41.0
17	38.508323978432244	40.0	38.0	41.0	34.0	41.0
18	38.42735364707202	40.0	38.0	41.0	34.0	41.0
19	38.38136101881779	40.0	37.0	41.0	34.0	41.0
20	38.32269846412196	40.0	37.0	41.0	34.0	41.0
21	38.25125548213946	40.0	37.0	41.0	34.0	41.0
22	38.186149556196796	40.0	37.0	41.0	34.0	41.0
23	38.01860264984375	40.0	36.0	41.0	34.0	41.0
24	37.788678853570474	40.0	35.0	41.0	33.0	41.0
25	37.667322974716235	40.0	35.0	41.0	33.0	41.0
26	37.59607420634024	40.0	35.0	41.0	33.0	41.0
27	37.540453773394574	40.0	35.0	41.0	33.0	41.0
28	37.423666099931275	39.0	35.0	41.0	33.0	41.0
29	37.352914585358135	39.0	35.0	41.0	33.0	41.0
30	37.212586883382265	39.0	35.0	41.0	33.0	41.0
31	37.07614931342979	40.0	35.0	41.0	32.0	41.0
32	36.833220739669216	40.0	35.0	41.0	31.0	41.0
33	36.552406281470766	40.0	35.0	41.0	30.0	41.0
34	36.22943072810848	39.0	35.0	41.0	30.0	41.0
35	35.97892363805616	39.0	35.0	41.0	27.0	41.0
36	35.82249127503744	39.0	35.0	41.0	26.0	41.0
37	35.74068716457343	39.0	35.0	41.0	26.0	41.0
38	35.616904452765496	39.0	35.0	41.0	25.0	41.0
39	35.50846629012666	39.0	35.0	41.0	25.0	41.0
40	35.395268219873245	39.0	35.0	41.0	24.0	41.0
41	35.29765662866944	39.0	35.0	40.0	24.0	41.0
42	35.21913405845327	39.0	35.0	40.0	23.0	41.0
43	35.09235610404157	38.0	35.0	40.0	23.0	41.0
44	34.89116001610633	38.0	34.0	40.0	23.0	41.0
45	34.7920650347701	38.0	34.0	40.0	23.0	41.0
46	34.74565970260204	38.0	34.0	40.0	23.0	41.0
47	34.6982087980438	38.0	34.0	40.0	23.0	41.0
48	34.59716163511114	38.0	34.0	40.0	23.0	41.0
49	34.47712758075979	38.0	34.0	40.0	22.0	41.0
50	34.37613650537729	37.0	34.0	40.0	22.0	41.0
51	34.002260244558464	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	1.0
13	4.0
14	10.0
15	26.0
16	67.0
17	166.0
18	390.0
19	874.0
20	1626.0
21	2776.0
22	4476.0
23	7314.0
24	11554.0
25	17555.0
26	22737.0
27	23487.0
28	22501.0
29	22314.0
30	24882.0
31	30081.0
32	39062.0
33	53059.0
34	80279.0
35	122170.0
36	130964.0
37	213038.0
38	255478.0
39	107643.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.37209234187287	20.514649314685478	26.201006059966797	19.91225228347485
2	38.16774530559762	23.077766643980507	26.172878572128173	12.581609478293698
3	28.54019175245132	23.505287716575378	34.7326758533051	13.221844677668198
4	25.673448237469664	25.859374280593457	33.576100341464354	14.891077140472525
5	22.321170706225967	29.77713151525958	32.74784627993046	15.153851498583998
6	20.602631426942615	37.94121857318295	31.22862708559881	10.227522914275621
7	79.77499683984325	4.809967845928337	13.043285357549761	2.371749956678646
8	29.840167224612223	63.94700647350784	4.908916329932084	1.3039099719478535
9	74.93999887824899	5.793425367143244	14.983077465278038	4.28349828932972
10	41.42651568233016	23.216813540706585	21.287066964349247	14.069603812614007
11	34.5036377380477	22.94014286419865	26.657156896968843	15.899062500784808
12	30.29179757249734	21.27333807147563	31.73148964347572	16.703374712551305
13	23.263525261581453	26.811188377989907	32.05261179630006	17.87267456412858
14	19.002796843359192	29.75904955879189	34.185864095680344	17.05228950216858
15	18.363733622644638	22.300577366915544	41.702349231223856	17.633339779215962
16	20.621634223785975	20.13593278200109	39.96062151702592	19.281811477187016
17	20.91663799504588	19.796979810993324	33.17093057616982	26.11545161779097
18	23.431034497191856	22.07304608136378	34.298625185319125	20.197294236125238
19	26.893812873515877	23.309650993126347	30.133078176836513	19.663457956521267
20	27.076222980659843	23.525546204840104	28.920163976557077	20.478066837942976
21	24.774708030816342	25.050541579710035	31.353777663928422	18.8209727255452
22	23.208358551802714	22.22431504125783	31.95517014200196	22.612156264937497
23	21.90076521835218	25.386062327499392	30.36337198351528	22.349800470633145
24	22.059317188490166	23.813769242424623	34.2862356966283	19.84067787245691
25	21.530503674571662	23.37126358553477	33.34731336449122	21.750919375402344
26	19.551199143451026	26.70646371344787	31.528653622544184	22.213683520556923
27	20.51414703811693	25.039910059009124	33.12430256805638	21.32164033481756
28	20.21763643715139	24.599832072200584	35.73547102240907	19.44706046823896
29	20.555752280544905	24.34492671366301	34.96003971333402	20.139281292458065
30	21.987324213665104	24.235430421719776	33.60640436110002	20.1708410035151
31	26.187528305377455	23.74077171446247	29.48999674357358	20.581703236586495
32	26.162330764188685	24.45492528217479	29.888971764522697	19.493772189113827
33	25.130822117916125	24.441614953108296	29.112368476787708	21.315194452187875
34	21.41129670230319	26.362571689516063	30.878456604560167	21.347675003620576
35	21.76146718334183	26.117042160258038	30.40280069414622	21.718689962253915
36	25.35358177606669	26.519281978902708	27.803100888108684	20.324035356921915
37	23.152019863364032	28.14900201831468	28.152183103248806	20.546795015072483
38	23.036663678121084	29.225882981279316	26.16450729598572	21.57294604461388
39	22.344442853901977	27.27495707628158	27.341759859898325	23.03884020991812
40	23.105308142489164	25.482415715898977	28.2974247443203	23.11485139729156
41	20.864736082962697	24.785255838755827	29.14133309224058	25.208674986040897
42	22.509775557715344	27.057136471055053	26.835046514995888	23.598041456233712
43	22.433010955489088	26.232398345500986	27.670332448489443	23.664258250520483
44	22.15692626831112	27.198192474055322	28.916229476770127	21.72865178086343
45	21.344075354879326	29.076706840420876	27.686070447637253	21.893147357062553
46	22.980157564159555	27.46297593844098	28.78195420744525	20.774912289954216
47	22.50818501524828	26.45951106724562	28.874121957773607	22.158181959732488
48	23.318022269268795	25.446168090202175	29.830791395332678	21.405018245196352
49	22.73923223677987	24.627875847277785	30.280998626273586	22.351893289668755
50	20.851509466657625	26.840990121057022	30.073307265179427	22.234193147105923
51	21.101224633986877	28.110912711866536	28.774420058917045	22.013442595229545
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1177.0
1	1989.5
2	2802.0
3	17948.0
4	33094.0
5	22254.5
6	11415.0
7	11133.0
8	10851.0
9	10960.0
10	11069.0
11	10825.0
12	10581.0
13	10234.5
14	9888.0
15	9440.5
16	8993.0
17	8717.0
18	8441.0
19	8009.0
20	7577.0
21	7518.0
22	7459.0
23	7529.0
24	7599.0
25	8329.5
26	10021.5
27	10983.0
28	12687.5
29	14392.0
30	16169.0
31	17946.0
32	19900.0
33	21854.0
34	23591.0
35	25328.0
36	28734.5
37	32141.0
38	40376.0
39	48611.0
40	57507.0
41	66403.0
42	72422.5
43	78442.0
44	80909.0
45	83376.0
46	84008.0
47	84640.0
48	83013.5
49	81387.0
50	78233.0
51	75079.0
52	71973.5
53	68868.0
54	66382.5
55	63897.0
56	61784.0
57	59671.0
58	57362.0
59	55053.0
60	50942.5
61	46832.0
62	41513.0
63	36194.0
64	31226.0
65	26258.0
66	22550.0
67	18842.0
68	16269.0
69	13696.0
70	11968.5
71	10241.0
72	8677.0
73	7113.0
74	5437.5
75	2760.5
76	1759.0
77	1322.5
78	886.0
79	694.0
80	502.0
81	364.5
82	227.0
83	166.0
84	105.0
85	73.0
86	41.0
87	25.5
88	10.0
89	7.5
90	5.0
91	3.5
92	2.0
93	4.0
94	6.0
95	3.0
96	0.0
97	1.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1194561.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.315803151938443
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.54504314108715	22.091746843512905
2	6.785039601746925	4.249579290928271
3	3.636924841353223	3.4167966723063743
4	2.5605563155223354	3.207435101454009
5	2.1038852098740257	3.2942427543344843
6	1.6903682890938416	3.1761144357325035
7	1.4867931053143502	3.2592084149578433
8	1.3111000933718044	3.2846521949215632
9	1.1323852830805092	3.1915399155391184
>10	8.663833166346175	46.4010385488361
>50	0.06043704978176312	1.2136138178684843
>100	0.022291068026785703	1.3071434193495335
>500	5.371341604601251E-4	0.10950332750483298
>1k	2.6856708023006255E-4	0.09512155407677889
>5k	2.6856708023006255E-4	0.6135382289921326
>10k+	2.6856708023006255E-4	1.0887254796851482
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12945	1.0836616966400208	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7295	0.6106845945916534	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00502276568546939	0.0
2	0.0	0.0	0.0	0.017663392660567354	0.0
3	0.0	0.0	0.0	0.035912774651106136	0.0
4	0.0	0.0	0.0	0.05876635851999186	0.0
5	0.0	0.0	0.0	0.11351450449160821	0.0
6	0.0	0.0	0.0	0.23121464705444092	0.0
7	0.0	0.0	0.0	0.3090675151792165	0.0
8	0.0	0.0	0.0	0.4800089740080247	0.0
9	0.0	0.0	0.0	0.7807052130447922	0.0
10	0.0	0.0	0.0	1.1596728840134576	0.0
11	0.0	0.0	0.0	1.3678665216761638	0.0
12	0.0	0.0	0.0	1.5497743522515803	0.0
13	0.0	0.0	0.0	1.6378401772701436	0.0
14	0.0	0.0	0.0	1.6817893770180008	0.0
15	0.0	0.0	0.0	1.7230597684002742	0.0
16	0.0	0.0	0.0	1.7839189459558784	0.0
17	0.0	0.0	0.0	1.8472057935927926	0.0
18	0.0	0.0	0.0	1.9589623300944865	0.0
19	8.371276142448984E-5	0.0	0.0	2.011533944269066	0.0
20	8.371276142448984E-5	0.0	0.0	2.0717234197332743	0.0
21	8.371276142448984E-5	0.0	0.0	2.1381913523043194	0.0
22	8.371276142448984E-5	0.0	0.0	2.2118585823578703	0.0
23	8.371276142448984E-5	0.0	0.0	2.2896277377212213	0.0
24	8.371276142448984E-5	0.0	0.0	2.354839978870899	0.0
25	8.371276142448984E-5	0.0	0.0	2.4095044120810907	0.0
26	8.371276142448984E-5	0.0	0.0	2.4691078982153276	0.0
27	8.371276142448984E-5	0.0	0.0	2.538840628481928	0.0
28	8.371276142448984E-5	0.0	0.0	2.609159348078499	0.0
29	8.371276142448984E-5	0.0	0.0	2.6854216737362093	0.0
30	8.371276142448984E-5	0.0	0.0	2.7825284769886176	0.0
31	8.371276142448984E-5	0.0	0.0	2.8599627813062707	0.0
32	8.371276142448984E-5	0.0	0.0	2.942252425786544	0.0
33	8.371276142448984E-5	0.0	0.0	3.022030687424083	0.0
34	8.371276142448984E-5	0.0	0.0	3.1064968637013934	0.0
35	8.371276142448984E-5	0.0	0.0	3.204524507329471	0.0
36	8.371276142448984E-5	0.0	0.0	3.2940134492922506	0.0
37	8.371276142448984E-5	0.0	0.0	3.3886925824633485	0.0
38	8.371276142448984E-5	0.0	0.0	3.5007002572493158	0.0
39	8.371276142448984E-5	0.0	0.0	3.6448536324222873	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATACG	20	7.0333976E-4	45.0	1
CGTTTTT	11140	0.0	43.54578	1
TTTAGCG	115	0.0	43.04348	1
GTTTTTA	7665	0.0	42.299416	2
TATACGA	55	6.184564E-11	40.909092	2
CGACGGT	195	0.0	40.384617	28
ATAACGC	95	0.0	40.263157	11
CGGTCTA	200	0.0	39.375004	31
TATGCGA	80	0.0	39.375	2
GCGTAAG	40	3.4583718E-7	39.375	1
GAGCGAT	970	0.0	38.96907	7
TATTTTT	8680	0.0	38.75288	7
TCGTTTA	70	0.0	38.571426	38
GCCGACC	70	0.0	38.571426	14
TTATTTT	8800	0.0	38.454544	6
CGTAGGA	65	9.094947E-12	38.07692	2
TTTTATT	8960	0.0	37.943638	4
AGGGGAA	6675	0.0	37.55056	8
CTCACGA	210	0.0	37.5	24
TACGCAA	30	1.139949E-4	37.499996	1
>>END_MODULE