FastQCFastQC Report
Thu 26 May 2016
SRR1546875_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546875_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2246254
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74290.33072840382254187No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC44570.19841923486836308No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41600.18519722168552621No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACAC207.0347276E-445.039
TATCGGC207.0347276E-445.033
ACCTACG302.166431E-644.99999619
TATTACG302.166431E-644.9999961
TCGACGA302.166431E-644.9999962
ACGTAGA2000.042.752
CGACGGT2750.042.54545628
TCACGAC2850.041.84210625
AGGGGAA218950.041.762968
GAGCGAT19350.041.2790687
AGCGATA4700.041.170218
GAGGGGA217150.040.8864867
TATGCGA2650.040.754722
CGTTTTT70400.040.7492871
CAATACG501.0822987E-940.51
ACGGTCT2950.040.4237330
CGGTCTA3000.039.7531
GTTTTTA53500.038.859812
TTACGAG4300.038.7209323
TAAGTCC356.2509534E-638.5714261
Produced by FastQC (version 0.11.5)