FastQCFastQC Report
Thu 26 May 2016
SRR1546874_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546874_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1355750
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184641.361903005716393No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102730.7577355707173151No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGA453.8562575E-1045.02
ATACGCG207.033737E-445.01
CGTTTTT171350.043.870731
GTTTTTA119500.042.7029272
TATGCGA556.184564E-1140.909092
AACCGAT451.9286745E-840.08
TATTTTT131750.039.5863387
TTATTTT132250.039.4366766
TTTTATT134400.039.224334
TTTATTT134650.038.917565
ATACGAG1450.038.7931023
ACGAGTA1000.038.255
ATAACGC1300.038.07692711
TTTTTAT138850.037.8700073
GAGCGAT11300.037.632747
TACGTAG900.037.51
GTTTCGT552.750312E-936.8181843
TTCGAAT800.036.562516
AGACTTA9250.036.4864858
GCGCGAC6750.036.3333329