##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546874_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1355750
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.64910492347409	31.0	31.0	34.0	30.0	34.0
2	32.00075603909276	31.0	31.0	34.0	30.0	34.0
3	31.889081320302417	31.0	31.0	34.0	30.0	34.0
4	35.73518126498248	37.0	35.0	37.0	35.0	37.0
5	35.763300756039094	37.0	35.0	37.0	35.0	37.0
6	35.81735570717315	37.0	35.0	37.0	35.0	37.0
7	36.05036179236585	37.0	35.0	37.0	35.0	37.0
8	13.268854877374148	0.0	0.0	35.0	0.0	37.0
9	24.887916651300017	18.0	17.0	38.0	17.0	39.0
10	32.53334538078554	32.0	32.0	37.0	27.0	39.0
11	35.50207855430573	37.0	34.0	37.0	32.0	39.0
12	36.69323179052185	38.0	35.0	39.0	33.0	39.0
13	37.00976654988014	39.0	37.0	39.0	33.0	39.0
14	38.23308353309976	40.0	37.0	41.0	33.0	41.0
15	38.4519800848239	40.0	38.0	41.0	34.0	41.0
16	38.50334132399041	40.0	38.0	41.0	34.0	41.0
17	38.45711746265904	40.0	38.0	41.0	34.0	41.0
18	38.360032454361054	40.0	38.0	41.0	34.0	41.0
19	38.28886962935645	40.0	37.0	41.0	34.0	41.0
20	38.19799889360133	40.0	37.0	41.0	34.0	41.0
21	38.14394394246727	40.0	37.0	41.0	33.0	41.0
22	38.09055799373041	40.0	36.0	41.0	33.0	41.0
23	37.8649367508759	39.0	36.0	41.0	33.0	41.0
24	37.53928821685414	39.0	35.0	41.0	33.0	41.0
25	37.41450488659414	39.0	35.0	41.0	33.0	41.0
26	37.34238687073576	39.0	35.0	41.0	33.0	41.0
27	37.28225484049419	39.0	35.0	41.0	33.0	41.0
28	37.12174589710492	39.0	35.0	41.0	33.0	41.0
29	37.01030352203578	39.0	35.0	41.0	32.0	41.0
30	36.833435367877556	39.0	35.0	41.0	32.0	41.0
31	36.653000921998895	39.0	35.0	41.0	31.0	41.0
32	36.29883901899318	39.0	35.0	41.0	30.0	41.0
33	35.893858012170384	39.0	35.0	41.0	27.0	41.0
34	35.44905034113959	39.0	35.0	41.0	24.0	41.0
35	35.11222127973446	39.0	35.0	41.0	22.0	41.0
36	34.9099081689102	39.0	35.0	41.0	21.0	41.0
37	34.83755928452886	39.0	35.0	41.0	20.0	41.0
38	34.70950396459524	39.0	34.0	40.0	18.0	41.0
39	34.60164484602618	38.0	34.0	40.0	18.0	41.0
40	34.48666125760649	38.0	34.0	40.0	18.0	41.0
41	34.37336160796607	38.0	34.0	40.0	18.0	41.0
42	34.28029208924949	38.0	34.0	40.0	18.0	41.0
43	34.14629762124285	38.0	34.0	40.0	17.0	41.0
44	33.946934169279	38.0	33.0	40.0	15.0	41.0
45	33.8511473354232	38.0	33.0	40.0	17.0	41.0
46	33.80660077447907	38.0	33.0	40.0	17.0	41.0
47	33.74799778720266	38.0	33.0	40.0	17.0	41.0
48	33.644936013276784	37.0	33.0	40.0	17.0	41.0
49	33.54811063986723	37.0	33.0	40.0	15.0	41.0
50	33.438134611838464	37.0	33.0	40.0	15.0	41.0
51	33.095127420247096	36.0	32.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	4.0
13	5.0
14	12.0
15	27.0
16	92.0
17	247.0
18	587.0
19	1292.0
20	2276.0
21	4009.0
22	6556.0
23	10671.0
24	16700.0
25	26602.0
26	34600.0
27	35994.0
28	32677.0
29	30791.0
30	32337.0
31	37284.0
32	46408.0
33	61463.0
34	89101.0
35	129444.0
36	145538.0
37	230710.0
38	263262.0
39	117050.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.899834040199153	20.524064171122994	24.44484602618477	23.131255762493087
2	41.495260925686885	21.601991517610177	24.869924396090724	12.032823160612208
3	27.74810990226812	22.564484602618474	36.94980638023234	12.737599114881062
4	24.742245989304813	24.437986354416374	36.30396459524249	14.515803061036328
5	21.752387977134426	28.054729854324172	35.967471879033745	14.225410289507654
6	19.457200811359026	36.6480545823345	33.84842338189194	10.04632122441453
7	76.85937672874793	4.166181080582703	16.818956297252445	2.155485893416928
8	28.804351834777798	63.63488843813387	6.267305919232897	1.2934538078554305
9	71.5863544163747	5.438465793841048	19.053291536050157	3.921888253734095
10	36.375954268854876	23.69315876820948	25.60936750875899	14.321519454176654
11	30.247980822423017	23.223529411764705	30.743794947446062	15.78469481836622
12	26.436289876452147	21.157661810805827	35.363820763415085	17.04222754932694
13	22.36533284160059	23.975659229208926	36.3783145860225	17.28069334316799
14	18.769758436289877	27.195869444956667	37.25546745343905	16.7789046653144
15	18.679328784805456	22.169205236953715	42.09360132767841	17.05786465056242
16	20.938152314217223	20.865203761755485	40.33730407523511	17.85933984879218
17	20.646874423750692	20.663027844366585	35.74729854324175	22.942799188640976
18	22.649382260741287	21.74921630094044	36.80818735017518	18.793214088143095
19	24.685967176839387	23.806453992255207	33.044440346671585	18.463138484233816
20	25.830868522957772	23.327162087405494	32.13232528120966	18.70964410842707
21	24.11565554121335	24.41482574220911	34.28014014383183	17.18937857274571
22	22.497068043518347	21.477042227549326	34.41593214088143	21.609957588050893
23	20.986391296330446	24.769832196201364	33.65598377281947	20.58779273464872
24	21.32568688917573	23.791111930665686	36.13446431864282	18.748736861515766
25	21.66453992255209	22.984030979162824	35.124174810990226	20.227254287294855
26	19.635183477779826	25.394947446063064	33.94681910381708	21.023049972340033
27	20.334206158952608	23.569463396643926	35.325686889175735	20.770643555227732
28	19.702231237322515	24.183588419693898	37.1280840862991	18.986096256684494
29	20.619140697031163	24.01239166513	35.844809146229025	19.52365849160981
30	22.059302968836437	22.88947077263507	35.52210953346856	19.529116725059932
31	25.26439240272912	22.526940807671032	32.443444587866495	19.76522220173336
32	24.391296330444405	23.62655356813572	32.768947077263505	19.21320302415637
33	23.915766181080585	23.421796053844734	31.97174995390005	20.690687811174627
34	21.156629172044994	25.254508574589714	33.10300571639314	20.485856536972154
35	21.412576064908723	25.463691683569976	32.875825189009774	20.247907062511526
36	23.816337820394615	26.747925502489394	29.789931772081875	19.645804905034115
37	22.084160059007928	27.403503595795687	29.999188640973628	20.513147704222757
38	21.79981560022128	29.164742762308688	28.11831089802692	20.917130739443113
39	21.589304812834225	26.670846394984327	28.623492531808964	23.116356260372488
40	22.896846763783884	25.385874976950028	29.769647796422642	21.947630462843446
41	20.68382813940623	25.473723031532362	29.952793656647614	23.889655172413793
42	22.249898580121705	27.28961829245805	28.197307763230683	22.263175364189564
43	21.957440531071363	26.09625668449198	29.229430204683755	22.716872579752902
44	21.93649271620874	27.053439055873135	29.595353125576253	21.414715102341876
45	21.22146413424304	27.63732251521298	29.44407154711414	21.697141803429837
46	22.788124654250417	26.809441268670476	29.470551355338376	20.93188272174073
47	21.97787202655357	26.52303153236216	30.030610363267563	21.468486077816706
48	21.897104923474092	26.2740918310898	30.518163378203944	21.310639867232158
49	22.01829245804905	25.116577540106956	30.80944126867048	22.055688733173522
50	20.519859856168175	26.10053475935829	31.530813203024156	21.848792181449383
51	20.869113037064356	27.383072100313477	30.160722847132583	21.58709201548958
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1013.0
1	1906.5
2	2800.0
3	25811.0
4	48822.0
5	33781.0
6	18740.0
7	18371.0
8	18002.0
9	17872.5
10	17743.0
11	17302.0
12	16861.0
13	16176.5
14	15492.0
15	14540.0
16	13588.0
17	12760.0
18	11932.0
19	11366.5
20	10801.0
21	10460.0
22	10119.0
23	9934.5
24	9750.0
25	10296.5
26	11779.0
27	12715.0
28	14586.0
29	16457.0
30	18327.0
31	20197.0
32	22498.0
33	24799.0
34	27566.5
35	30334.0
36	34244.5
37	38155.0
38	44469.5
39	50784.0
40	57914.5
41	65045.0
42	71354.0
43	77663.0
44	82972.0
45	88281.0
46	92089.0
47	95897.0
48	95807.0
49	95717.0
50	93415.5
51	91114.0
52	84962.5
53	78811.0
54	74708.5
55	70606.0
56	67923.0
57	65240.0
58	61598.0
59	57956.0
60	53166.5
61	48377.0
62	43152.0
63	37927.0
64	32292.0
65	26657.0
66	22805.0
67	18953.0
68	16291.5
69	13630.0
70	11833.0
71	10036.0
72	8366.5
73	6697.0
74	5229.5
75	2783.5
76	1805.0
77	1331.0
78	857.0
79	630.0
80	403.0
81	301.0
82	199.0
83	145.0
84	91.0
85	67.0
86	43.0
87	30.5
88	18.0
89	12.5
90	7.0
91	6.5
92	6.0
93	4.5
94	3.0
95	1.5
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1355750.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.81087591857182
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.65598510529448	27.03409794907471
2	8.404701393357485	6.523876458204899
3	4.728432949770665	5.505438735085074
4	3.3177169389799257	5.150540018067751
5	2.608612346544428	5.062126505069514
6	2.0719031063832003	4.824742462628513
7	1.7058186298092601	4.634302062678078
8	1.3868746800867804	4.3060656898765615
9	1.1377747956739541	3.9742232776362205
>10	4.927501581752474	28.0601526426237
>50	0.03368000091515266	0.9145640462464512
>100	0.019248601090271003	1.502409786447468
>500	0.001361010265940526	0.338966323563569
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	3.8886007598300747E-4	2.1684940427974806
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18464	1.361903005716393	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10273	0.7577355707173151	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.375991148810622E-5	0.0	0.0	0.009662548404941913	0.0
2	7.375991148810622E-5	0.0	0.0	0.03791259450488659	0.0
3	7.375991148810622E-5	0.0	0.0	0.07538262954084456	0.0
4	7.375991148810622E-5	0.0	0.0	0.11233634519638576	0.0
5	7.375991148810622E-5	0.0	0.0	0.21412502304997233	0.0
6	7.375991148810622E-5	0.0	0.0	0.4274386870735755	0.0
7	7.375991148810622E-5	0.0	0.0	0.5543057348331182	0.0
8	7.375991148810622E-5	0.0	0.0	0.8075972708832749	0.0
9	7.375991148810622E-5	0.0	0.0	1.206859671768394	0.0
10	7.375991148810622E-5	0.0	0.0	1.7518716577540108	0.0
11	7.375991148810622E-5	0.0	0.0	2.05568873317352	0.0
12	7.375991148810622E-5	0.0	0.0	2.310455467453439	0.0
13	7.375991148810622E-5	0.0	0.0	2.4671215194541767	0.0
14	7.375991148810622E-5	0.0	0.0	2.554158215010142	0.0
15	7.375991148810622E-5	0.0	0.0	2.6234187718974735	0.0
16	7.375991148810622E-5	0.0	0.0	2.7273464871842155	0.0
17	7.375991148810622E-5	0.0	0.0	2.825594689286373	0.0
18	7.375991148810622E-5	0.0	0.0	3.008814309422829	0.0
19	7.375991148810622E-5	0.0	0.0	3.085377097547483	0.0
20	7.375991148810622E-5	0.0	0.0	3.169905956112853	0.0
21	7.375991148810622E-5	0.0	0.0	3.256057532730961	0.0
22	7.375991148810622E-5	0.0	0.0	3.353420615895261	0.0
23	7.375991148810622E-5	0.0	0.0	3.4587497695002765	0.0
24	7.375991148810622E-5	0.0	0.0	3.5539000553199336	0.0
25	7.375991148810622E-5	0.0	0.0	3.63894523326572	0.0
26	7.375991148810622E-5	0.0	0.0	3.726645768025078	0.0
27	7.375991148810622E-5	0.0	0.0	3.8231237322515215	0.0
28	7.375991148810622E-5	0.0	0.0	3.9233634519638576	0.0
29	7.375991148810622E-5	0.0	0.0	4.028987645214825	0.0
30	7.375991148810622E-5	0.0	0.0	4.165074681910382	0.0
31	7.375991148810622E-5	0.0	0.0	4.277853586575696	0.0
32	7.375991148810622E-5	0.0	0.0	4.410695187165776	0.0
33	7.375991148810622E-5	0.0	0.0	4.545307025631569	0.0
34	7.375991148810622E-5	0.0	0.0	4.68471325834409	0.0
35	7.375991148810622E-5	0.0	0.0	4.8198414161903	0.0
36	7.375991148810622E-5	0.0	0.0	4.945897104923474	0.0
37	7.375991148810622E-5	0.0	0.0	5.075198229762124	0.0
38	1.4751982297621244E-4	0.0	0.0	5.226627328047206	0.0
39	1.4751982297621244E-4	0.0	0.0	5.418993177208187	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACGA	45	3.8562575E-10	45.0	2
ATACGCG	20	7.033737E-4	45.0	1
CGTTTTT	17135	0.0	43.87073	1
GTTTTTA	11950	0.0	42.702927	2
TATGCGA	55	6.184564E-11	40.90909	2
AACCGAT	45	1.9286745E-8	40.0	8
TATTTTT	13175	0.0	39.586338	7
TTATTTT	13225	0.0	39.436676	6
TTTTATT	13440	0.0	39.22433	4
TTTATTT	13465	0.0	38.91756	5
ATACGAG	145	0.0	38.793102	3
ACGAGTA	100	0.0	38.25	5
ATAACGC	130	0.0	38.076927	11
TTTTTAT	13885	0.0	37.870007	3
GAGCGAT	1130	0.0	37.63274	7
TACGTAG	90	0.0	37.5	1
GTTTCGT	55	2.750312E-9	36.81818	43
TTCGAAT	80	0.0	36.5625	16
AGACTTA	925	0.0	36.486485	8
GCGCGAC	675	0.0	36.333332	9
>>END_MODULE