FastQCFastQC Report
Thu 26 May 2016
SRR1546873_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546873_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1056261
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169811.6076518966429698No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94680.8963693632539685No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGG207.0330274E-445.02
TACGTAG253.89059E-545.01
CCGGTAT302.165334E-644.99999642
CGTTTTT135250.043.6192251
GTTTTTA93200.042.537552
CGCATCG900.042.521
TGTACGA800.042.18752
CGTATGA603.6379788E-1241.2499962
GCGATAT501.0822987E-940.59
CGGTCTA1450.040.34482631
TTACGAG1350.040.0000043
GCGATAC451.9281288E-840.09
TATTTTT104250.039.6043137
ATAACGC1250.039.611
TTATTTT104600.039.4502836
TTTATTT105100.039.24125
AGACTTA5850.039.2307668
AGCGATA1900.039.078958
ATTGCGA750.039.02
TTTTATT105850.038.9844134
Produced by FastQC (version 0.11.5)