FastQCFastQC Report
Thu 26 May 2016
SRR1546872_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546872_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences981888
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165841.6889910050840828No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90960.9263785686351192No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG253.8903898E-545.01
ATATGCG207.0327846E-445.01
TATTACG207.0327846E-445.01
ATCGCGA253.8903898E-545.02
TTAAGCG302.1651776E-644.9999961
CGTTTTT145750.043.7804451
GTTTTTA94450.042.998942
ATAACGC1050.042.85714311
TATGCGA900.042.52
CGACGGT1150.041.0869628
CACGACG1150.041.0869626
CGGTCTA1150.041.0869631
ACGTTAG501.0804797E-940.51
TATACGA501.0804797E-940.52
TATTTTT107600.039.5004657
GGTACGA403.457535E-739.3752
CGTAGGA403.457535E-739.3752
TCACGAC1200.039.37499625
GACGGTC1200.039.37499629
TTATTTT107950.039.3515556
Produced by FastQC (version 0.11.5)