##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546872_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	981888
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.644083642940945	31.0	31.0	34.0	30.0	34.0
2	31.99156013720506	31.0	31.0	34.0	30.0	34.0
3	31.82846210565767	31.0	31.0	34.0	30.0	34.0
4	35.709802951049404	37.0	35.0	37.0	35.0	37.0
5	35.752288448377	37.0	35.0	37.0	35.0	37.0
6	35.80565298689871	37.0	35.0	37.0	35.0	37.0
7	36.02766710663538	37.0	35.0	37.0	35.0	37.0
8	13.26417575120584	0.0	0.0	35.0	0.0	37.0
9	24.858562280015644	18.0	17.0	38.0	17.0	39.0
10	32.5971006876548	32.0	32.0	37.0	27.0	39.0
11	35.53601429083562	37.0	35.0	37.0	32.0	39.0
12	36.71794033535393	38.0	35.0	39.0	33.0	39.0
13	37.033000708838486	39.0	37.0	39.0	33.0	39.0
14	38.25432737746056	40.0	37.0	41.0	33.0	41.0
15	38.45701240874723	40.0	38.0	41.0	34.0	41.0
16	38.48579573230348	40.0	38.0	41.0	34.0	41.0
17	38.40393812736279	40.0	38.0	41.0	33.0	41.0
18	38.278808784708644	40.0	37.0	41.0	33.0	41.0
19	38.19253112371268	40.0	37.0	41.0	34.0	41.0
20	38.091254807065575	40.0	36.0	41.0	34.0	41.0
21	38.056296644831185	40.0	36.0	41.0	33.0	41.0
22	37.99776349237388	40.0	36.0	41.0	33.0	41.0
23	37.76886976926085	39.0	36.0	41.0	33.0	41.0
24	37.37422598096728	39.0	35.0	41.0	33.0	41.0
25	37.248902115108855	39.0	35.0	41.0	33.0	41.0
26	37.16796009320819	39.0	35.0	41.0	33.0	41.0
27	37.12192225589884	39.0	35.0	41.0	33.0	41.0
28	36.92277428790249	39.0	35.0	41.0	33.0	41.0
29	36.79921335223569	39.0	35.0	41.0	32.0	41.0
30	36.57929315767175	39.0	35.0	41.0	31.0	41.0
31	36.35999523367227	39.0	35.0	41.0	31.0	41.0
32	35.92956834180681	39.0	35.0	41.0	29.0	41.0
33	35.42622580172077	39.0	35.0	41.0	24.0	41.0
34	34.88769289369052	39.0	35.0	41.0	21.0	41.0
35	34.48129827434494	38.0	34.0	41.0	18.0	41.0
36	34.274280773367224	38.0	34.0	40.0	16.0	41.0
37	34.19748688241429	38.0	34.0	40.0	15.0	41.0
38	34.05379839655847	38.0	34.0	40.0	15.0	41.0
39	33.92881265480381	38.0	33.0	40.0	15.0	41.0
40	33.80512645026724	38.0	33.0	40.0	15.0	41.0
41	33.68074464704732	38.0	33.0	40.0	13.0	41.0
42	33.59447411517403	38.0	33.0	40.0	12.0	41.0
43	33.46112998631209	38.0	33.0	40.0	12.0	41.0
44	33.214639551557816	37.0	33.0	40.0	10.0	41.0
45	33.15016172924	37.0	33.0	40.0	10.0	41.0
46	33.126774133098685	37.0	33.0	40.0	10.0	41.0
47	33.08981370584018	37.0	32.0	40.0	11.0	41.0
48	33.00094919176118	37.0	32.0	40.0	11.0	41.0
49	32.89347461217573	36.0	32.0	40.0	10.0	41.0
50	32.795351404640854	36.0	32.0	40.0	10.0	41.0
51	32.464497987550516	36.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	2.0
12	3.0
13	3.0
14	13.0
15	18.0
16	61.0
17	188.0
18	475.0
19	1030.0
20	1876.0
21	3418.0
22	5501.0
23	8899.0
24	14728.0
25	22400.0
26	29125.0
27	30286.0
28	27060.0
29	24245.0
30	24728.0
31	28080.0
32	34156.0
33	45619.0
34	65261.0
35	92783.0
36	102427.0
37	160374.0
38	180256.0
39	78855.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.540244915917093	20.065832355625083	23.45858183418068	25.935340894277147
2	43.953281840698736	21.101693879546342	23.100190653109113	11.844833626645809
3	26.914067592230477	22.06850475818016	38.65970456915656	12.357723080432798
4	24.2261846564985	23.51388345717638	38.33716269065311	13.92276919567201
5	21.303549895711118	26.679723145613348	37.8915925237909	14.12513443488463
6	19.330717963759614	34.647230641376616	36.20392549863121	9.818125896232564
7	73.14724286272977	4.257613903011341	20.234487029070525	2.3606562051883717
8	27.49203575153174	63.73802307391474	7.428240288098031	1.3417008864554816
9	67.51146770303741	5.9362167579194365	21.986112469039238	4.566203070003911
10	35.77852056446356	23.492088710728716	27.33091757919437	13.398473145613348
11	29.542676965193586	21.872759418589492	33.31265887759093	15.271904738625993
12	26.636235497327597	18.836262384304522	37.77538782427324	16.752114294094643
13	22.816858949289532	20.948417742145743	38.26414010559249	17.970583202972232
14	18.45108607091644	24.52591334245861	39.64780097119019	17.375199615434756
15	18.569225818015905	20.808686937817754	43.40383017207665	17.21825707208969
16	21.980307326293833	19.05156188893234	41.58997767566158	17.37815310911224
17	20.96155569678008	19.68177633294225	38.33431104158519	21.022356928692478
18	22.90342686742276	20.897088058923217	37.9171555207926	18.282329552861427
19	24.422133685308303	23.403993123451965	34.46828966236475	17.705583528874982
20	25.508204601746836	22.615817690001304	34.14859943292922	17.727378275322643
21	24.056307847738235	23.37710614652588	35.87608770043019	16.690498305305695
22	21.609592947464478	20.633819743188635	37.13641474384044	20.620172565506454
23	21.556328216660148	23.005577010819973	36.005735888410904	19.432358884108982
24	22.301219691044192	21.760526658845	37.301606700560555	18.636646949550254
25	22.20334702124886	21.647988365271804	35.74480999869639	20.403854614782947
26	19.272259157867293	23.21537690653109	36.30607564202842	21.2062882935732
27	19.947692608525617	21.434012840568375	37.12103620779559	21.497258343110413
28	19.460569840959458	23.427824762090992	37.967059379481164	19.144546017468386
29	21.430040900795202	23.28310357189415	36.93883620127754	18.34801932603311
30	21.86899116803546	22.076244948507366	37.45131827662625	18.60344560683092
31	24.567975166210402	21.459168459131796	34.394961543475425	19.577894831182373
32	25.103881501759872	22.763696063094773	34.119879253030895	18.012543182114456
33	24.176993710076914	22.822867781254075	32.98746903923869	20.01266946943032
34	21.139580074305826	25.240760657019944	34.785026398122795	18.834632870551427
35	21.557957730413243	25.302172956589754	33.30277995046278	19.83708936253422
36	22.96494101160214	27.634210826489376	30.422919925694174	18.977928236214314
37	23.240532525094512	27.542754367096855	29.70389698214053	19.5128161256681
38	22.692710370225523	28.336429409464216	28.587272682831443	20.383587537478817
39	23.301028223178204	25.79825804979794	28.55142338026333	22.349290346760526
40	22.926138215356538	24.433540281579976	30.822354484421847	21.81796701864164
41	20.823556250814757	25.529795658975363	30.398171685569025	23.248476404640854
42	21.909932701081996	27.111238756355107	28.508241265806284	22.470587276756614
43	21.54746773562769	25.615345131012905	29.512938339199586	23.32424879415982
44	22.043756518055012	25.95713564072481	30.183585093208187	21.815522748011993
45	21.708484063355495	26.23955074305827	29.92255735888411	22.129407834702125
46	22.165358981879805	26.812630361100247	30.059130980315473	20.96287967670447
47	21.510192608525617	26.410955220962062	30.800967116412465	21.27788505409986
48	21.478315245730677	25.28119296701864	31.760750716986053	21.479741070264634
49	22.09416959979142	24.5143030569678	31.81197855559901	21.579548787641766
50	20.40253063485856	24.728482270890368	32.61573621431365	22.25325087993743
51	20.40589150697432	25.740512156172596	32.28423201016817	21.569364326684916
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	735.0
1	1636.0
2	2537.0
3	23991.5
4	45446.0
5	31049.0
6	16652.0
7	16250.5
8	15849.0
9	15794.0
10	15739.0
11	15135.0
12	14531.0
13	13789.0
14	13047.0
15	12161.0
16	11275.0
17	10562.0
18	9849.0
19	9221.0
20	8593.0
21	8215.0
22	7837.0
23	7590.5
24	7344.0
25	7726.0
26	8791.5
27	9475.0
28	10411.0
29	11347.0
30	12633.0
31	13919.0
32	15400.5
33	16882.0
34	18699.5
35	20517.0
36	22651.0
37	24785.0
38	28046.5
39	31308.0
40	35424.5
41	39541.0
42	42865.5
43	46190.0
44	50499.5
45	54809.0
46	59101.0
47	63393.0
48	62886.5
49	62380.0
50	60605.5
51	58831.0
52	55769.0
53	52707.0
54	52016.0
55	51325.0
56	51229.0
57	51133.0
58	51099.5
59	51066.0
60	47016.0
61	42966.0
62	37723.5
63	32481.0
64	27592.5
65	22704.0
66	19216.5
67	15729.0
68	13390.0
69	11051.0
70	9616.0
71	8181.0
72	6822.5
73	5464.0
74	4271.0
75	2360.0
76	1642.0
77	1195.0
78	748.0
79	569.5
80	391.0
81	278.5
82	166.0
83	121.5
84	77.0
85	54.0
86	31.0
87	23.0
88	15.0
89	12.0
90	9.0
91	6.0
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	981888.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.83385923718591
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.64431725027859	32.1487829153228
2	10.972499174556939	10.277689636426766
3	6.443624166520416	9.053393615764397
4	4.179920059906574	7.830471508334168
5	2.9071641753231607	6.8076858883237295
6	1.9819906786918948	5.569456347316247
7	1.3718059763869341	4.497287759918525
8	0.9582701520134561	3.5903591528475958
9	0.6568820165698717	2.7687887909524975
>10	1.8327007268885305	11.867505736813957
>50	0.029358426271844346	0.9676756293146075
>100	0.020590984529437615	1.8654118604717398
>500	4.381060312042241E-4	0.12095476657766487
>1k	0.0	0.0
>5k	2.1905301560211206E-4	0.9331675630113991
>10k+	2.1905301560211206E-4	1.701368828603897
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16584	1.6889910050840828	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9096	0.9263785686351192	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.002953493677486638	0.0
2	0.0	0.0	0.0	0.02505377395385217	0.0
3	0.0	0.0	0.0	0.06497686090470603	0.0
4	0.0	0.0	0.0	0.10836266458088906	0.0
5	0.0	0.0	0.0	0.22792823621431366	0.0
6	0.0	0.0	0.0	0.4159333854777734	0.0
7	0.0	0.0	0.0	0.5450723504106375	0.0
8	0.0	0.0	0.0	0.760473699648025	0.0
9	0.0	0.0	0.0	1.1910727089036632	0.0
10	0.0	0.0	0.0	1.7603840763916048	0.0
11	0.0	0.0	0.0	2.0423918002867945	0.0
12	0.0	0.0	0.0	2.2823377004301917	0.0
13	0.0	0.0	0.0	2.439993156042237	0.0
14	0.0	0.0	0.0	2.526153695737192	0.0
15	0.0	0.0	0.0	2.5896028874983705	0.0
16	0.0	0.0	0.0	2.664967898579064	0.0
17	0.0	2.0368921913700952E-4	0.0	2.734222233085647	0.0
18	0.0	2.0368921913700952E-4	0.0	2.8658054686481553	0.0
19	0.0	2.0368921913700952E-4	0.0	2.9272177682179636	0.0
20	0.0	2.0368921913700952E-4	0.0	3.0002403532785817	0.0
21	0.0	2.0368921913700952E-4	0.0	3.0625692543345067	0.0
22	0.0	2.0368921913700952E-4	0.0	3.139360089949159	0.0
23	0.0	2.0368921913700952E-4	0.0	3.222668980576196	0.0
24	0.0	2.0368921913700952E-4	0.0	3.288867976795724	0.0
25	0.0	2.0368921913700952E-4	0.0	3.3504839655846697	0.0
26	0.0	2.0368921913700952E-4	0.0	3.416581117194629	0.0
27	0.0	2.0368921913700952E-4	0.0	3.4987697171164123	0.0
28	0.0	2.0368921913700952E-4	0.0	3.5744402620258113	0.0
29	0.0	2.0368921913700952E-4	0.0	3.6503144961543477	0.0
30	0.0	2.0368921913700952E-4	0.0	3.748085321340112	0.0
31	0.0	2.0368921913700952E-4	0.0	3.833227414939382	0.0
32	0.0	2.0368921913700952E-4	0.0	3.93079455090601	0.0
33	0.0	2.0368921913700952E-4	0.0	4.025306348585582	0.0
34	0.0	2.0368921913700952E-4	0.0	4.123790086038326	0.0
35	0.0	2.0368921913700952E-4	0.0	4.230523236866119	0.0
36	0.0	2.0368921913700952E-4	0.0	4.335423184721679	0.0
37	0.0	2.0368921913700952E-4	0.0	4.44297109242602	0.0
38	0.0	2.0368921913700952E-4	0.0	4.587692282622865	0.0
39	0.0	2.0368921913700952E-4	0.0	4.762253943423283	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	25	3.8903898E-5	45.0	1
ATATGCG	20	7.0327846E-4	45.0	1
TATTACG	20	7.0327846E-4	45.0	1
ATCGCGA	25	3.8903898E-5	45.0	2
TTAAGCG	30	2.1651776E-6	44.999996	1
CGTTTTT	14575	0.0	43.780445	1
GTTTTTA	9445	0.0	42.99894	2
ATAACGC	105	0.0	42.857143	11
TATGCGA	90	0.0	42.5	2
CGACGGT	115	0.0	41.08696	28
CACGACG	115	0.0	41.08696	26
CGGTCTA	115	0.0	41.08696	31
ACGTTAG	50	1.0804797E-9	40.5	1
TATACGA	50	1.0804797E-9	40.5	2
TATTTTT	10760	0.0	39.500465	7
GGTACGA	40	3.457535E-7	39.375	2
CGTAGGA	40	3.457535E-7	39.375	2
TCACGAC	120	0.0	39.374996	25
GACGGTC	120	0.0	39.374996	29
TTATTTT	10795	0.0	39.351555	6
>>END_MODULE