FastQCFastQC Report
Thu 26 May 2016
SRR1546871_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546871_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences920413
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96891.0526796123044764No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55660.604728529475355No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATCG207.0325535E-445.044
TAGTACG253.890198E-545.01
CGTTTTT78750.043.457141
CGCATCG900.042.50000421
CGAGCTT800.042.18756
GTTTTTA55950.041.702412
GTATGCG603.6379788E-1241.2499961
CGAGTAC556.002665E-1140.9090926
GAGCGAT9250.040.8648647
TCACGAC1450.040.3448325
TATGCGA850.039.7058832
GCGATCG850.039.7058839
CTATGCG403.4572258E-739.3751
CACGACG1450.038.79310626
TATTTTT63550.038.6978727
TAACGAG1400.038.571433
TATGACG356.2469317E-638.571431
TAAGCGA700.038.571432
TTATTTT63700.038.430146
TAACGCC1250.037.812
Produced by FastQC (version 0.11.5)