##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546870_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1498599
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.678532415943156	31.0	31.0	34.0	30.0	34.0
2	32.02305820302829	31.0	31.0	34.0	30.0	34.0
3	31.930032650495562	31.0	31.0	34.0	30.0	34.0
4	35.77116226555603	37.0	35.0	37.0	35.0	37.0
5	35.80531883445805	37.0	35.0	37.0	35.0	37.0
6	35.85415845065958	37.0	35.0	37.0	35.0	37.0
7	36.06589354457063	37.0	35.0	37.0	35.0	37.0
8	13.085411107307559	0.0	0.0	35.0	0.0	37.0
9	24.78324354947521	18.0	17.0	38.0	17.0	39.0
10	32.599835579764836	32.0	32.0	37.0	27.0	39.0
11	35.55444184868667	37.0	35.0	37.0	32.0	39.0
12	36.70553163321209	38.0	35.0	39.0	33.0	39.0
13	36.995540501495064	39.0	37.0	39.0	33.0	39.0
14	38.22418405457364	40.0	37.0	41.0	33.0	41.0
15	38.47142297572599	40.0	38.0	41.0	34.0	41.0
16	38.52300715534977	40.0	38.0	41.0	34.0	41.0
17	38.48554149575704	40.0	38.0	41.0	34.0	41.0
18	38.380008261049156	40.0	38.0	41.0	34.0	41.0
19	38.30213285875674	40.0	37.0	41.0	34.0	41.0
20	38.22891780923383	40.0	37.0	41.0	34.0	41.0
21	38.17202000001335	40.0	37.0	41.0	34.0	41.0
22	38.10766322411799	40.0	36.0	41.0	33.0	41.0
23	37.895745292770115	39.0	36.0	41.0	33.0	41.0
24	37.577468689088946	39.0	35.0	41.0	33.0	41.0
25	37.450183137717296	39.0	35.0	41.0	33.0	41.0
26	37.36655836551339	39.0	35.0	41.0	33.0	41.0
27	37.29828926884377	39.0	35.0	41.0	33.0	41.0
28	37.147034663709235	39.0	35.0	41.0	33.0	41.0
29	37.05479984972631	39.0	35.0	41.0	33.0	41.0
30	36.888809481388954	39.0	35.0	41.0	32.0	41.0
31	36.699063592061655	39.0	35.0	41.0	31.0	41.0
32	36.36851686141523	39.0	35.0	41.0	30.0	41.0
33	35.97609900980849	39.0	35.0	41.0	28.0	41.0
34	35.55561761351769	39.0	35.0	41.0	25.0	41.0
35	35.20401721874898	39.0	35.0	41.0	23.0	41.0
36	35.01086748356298	39.0	35.0	41.0	21.0	41.0
37	34.894445412014825	39.0	35.0	41.0	21.0	41.0
38	34.77641717364018	39.0	34.0	40.0	20.0	41.0
39	34.65190487915713	38.0	34.0	40.0	19.0	41.0
40	34.543417551993564	38.0	34.0	40.0	18.0	41.0
41	34.43980411037242	38.0	34.0	40.0	18.0	41.0
42	34.364657923834194	38.0	34.0	40.0	18.0	41.0
43	34.24788085405102	38.0	34.0	40.0	18.0	41.0
44	34.05880492379883	38.0	33.0	40.0	18.0	41.0
45	33.96892964695692	38.0	33.0	40.0	18.0	41.0
46	33.92958690083204	38.0	33.0	40.0	18.0	41.0
47	33.88678092004599	37.0	33.0	40.0	18.0	41.0
48	33.79522006887767	37.0	33.0	40.0	18.0	41.0
49	33.68044420155091	37.0	33.0	40.0	18.0	41.0
50	33.580614293750365	37.0	33.0	40.0	18.0	41.0
51	33.22525905862742	36.0	33.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	3.0
11	2.0
12	8.0
13	5.0
14	8.0
15	36.0
16	96.0
17	276.0
18	598.0
19	1282.0
20	2571.0
21	4366.0
22	7075.0
23	11328.0
24	17965.0
25	27639.0
26	36085.0
27	38278.0
28	35435.0
29	32800.0
30	34702.0
31	40678.0
32	51081.0
33	68166.0
34	101251.0
35	150553.0
36	160784.0
37	256662.0
38	294712.0
39	124128.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.548198684237743	21.21721688056645	24.57448590316689	23.660098532028915
2	39.24205207663958	22.816043517979125	26.099577004922597	11.842327400458695
3	27.598777257958933	23.07014751778161	37.32679656132161	12.00427866293785
4	24.607983856922367	24.530911871688158	36.65610346730513	14.205000804084348
5	21.92360998505938	28.60171400087682	35.69433851217036	13.780337501893436
6	19.970252215569342	36.376842637690274	33.47873580590939	10.174169340831003
7	76.852513581018	4.459965607877757	16.43715230024843	2.250368510855806
8	28.53318332656034	64.22351809923802	6.028497283129109	1.2148012910725285
9	72.13464042081971	5.826241709756913	18.079552969139844	3.959564900283531
10	39.42475605548916	23.637544132886784	24.05306556323606	12.884634248387993
11	32.46805849997231	22.15195659412558	30.07021891780256	15.309765988099553
12	28.3417378498184	20.449499832843877	34.512968445861766	16.69579387147596
13	22.144216031106385	25.748449051414017	34.9667255883662	17.140609329113392
14	17.74303866477957	28.79743013307763	36.69847637693606	16.76105482520674
15	17.41439838142158	21.779475363322675	43.94437738180794	16.8617488734478
16	19.776004121182517	19.925410333251257	41.78295861668131	18.515626928884913
17	20.022501015948897	19.494607963838227	35.74358450793041	24.73930651228247
18	22.172709310496003	21.593434934895857	36.838073427247714	19.395782327360422
19	25.443564289045966	22.95423925946834	32.85755562361913	18.74464082786656
20	25.971390612165095	23.015429744714897	31.58543412880964	19.427745514310367
21	23.292154872651054	24.18538915346934	34.06955429704677	18.452901676832827
22	21.967317474521202	21.62813401049914	33.99181502189712	22.41273349308254
23	20.6865212108109	24.504153546078705	33.22623330190398	21.58309194120642
24	21.102576473092537	22.9637147762677	36.90513606375021	19.028572686889554
25	21.0844929163839	23.02370413966645	35.685063182345644	20.206739761604002
26	19.022300161684345	25.990141458789175	33.629409868817476	21.358148510709
27	20.13166964611614	24.037250792239952	35.24491875411634	20.586160807527566
28	19.200533298100424	24.22696131520173	37.830466989501524	18.742038397196314
29	20.139476938126876	23.754253139098587	36.78001920460376	19.32625071817077
30	21.038783557175737	23.690927326122598	35.60291979375403	19.667369322947632
31	24.99614640073829	23.164569040817458	31.73217118121659	20.107113377227666
32	25.21455039006432	23.964849836413876	31.8123794290534	19.0082203444684
33	24.549329073354514	24.13587624174312	30.791158942452252	20.523635742450118
34	21.35207617247843	25.870830021907125	32.26353414088759	20.513559664726856
35	21.71628300832978	25.126601579208312	32.30397190976372	20.853143502698188
36	25.576955543143963	25.447968402487923	29.580561577847043	19.39451447652107
37	22.786882948674062	27.60978754156382	29.816181646991623	19.787147862770492
38	22.877033816251043	28.509494534561945	27.52250602062326	21.090965628563747
39	22.24177381674484	26.60197958226317	29.132876773573184	22.023369827418808
40	23.261192620574285	24.869961877727132	29.947304115377094	21.92154138632149
41	20.448498897970705	24.656562562766958	30.492079602348593	24.402858936913745
42	22.428081161137836	27.238574161600265	27.961649513979392	22.371695163282507
43	22.072549094187305	26.185257030066083	29.214753246198615	22.527440629548
44	22.17044052478348	26.91307014084488	29.88844914483461	21.028040189537027
45	20.674309805358206	28.47372779509395	29.277345040267612	21.574617359280236
46	22.741307047448984	27.200872281377475	29.632410004277325	20.425410666896216
47	21.994743090046104	26.72609550653644	30.103783600549576	21.175377802867878
48	22.663234127341603	25.39632016303227	30.496283528815916	21.44416218081021
49	21.89731876239074	24.285349182803408	31.750655111874494	22.066676942931366
50	20.651555219241438	26.63807996668889	30.970326284749955	21.74003852931972
51	20.37569756819536	27.98867475555502	29.738909474782783	21.896718201466836
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1607.0
1	2748.0
2	3889.0
3	30172.5
4	56456.0
5	38459.5
6	20463.0
7	19930.5
8	19398.0
9	19213.0
10	19028.0
11	18605.5
12	18183.0
13	17359.0
14	16535.0
15	15500.5
16	14466.0
17	13587.0
18	12708.0
19	12087.0
20	11466.0
21	11323.0
22	11180.0
23	10925.0
24	10670.0
25	11429.0
26	13705.0
27	15222.0
28	17486.5
29	19751.0
30	21833.0
31	23915.0
32	26272.0
33	28629.0
34	30506.5
35	32384.0
36	36331.5
37	40279.0
38	48717.0
39	57155.0
40	67168.5
41	77182.0
42	82471.5
43	87761.0
44	90008.5
45	92256.0
46	95061.0
47	97866.0
48	100650.5
49	103435.0
50	101324.0
51	99213.0
52	91852.0
53	84491.0
54	79416.0
55	74341.0
56	72146.0
57	69951.0
58	67182.5
59	64414.0
60	59816.0
61	55218.0
62	49550.5
63	43883.0
64	37924.0
65	31965.0
66	27681.0
67	23397.0
68	20346.0
69	17295.0
70	15046.5
71	12798.0
72	10681.5
73	8565.0
74	6505.0
75	3377.0
76	2309.0
77	1765.0
78	1221.0
79	885.5
80	550.0
81	417.5
82	285.0
83	198.5
84	112.0
85	76.0
86	40.0
87	30.0
88	20.0
89	14.0
90	8.0
91	6.5
92	5.0
93	3.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1498599.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.8151908868831
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.75861881769283	23.547727742816722
2	6.774083673212508	4.445856976403735
3	3.643871237011915	3.587229906293065
4	2.6009989828635516	3.4140911247702492
5	1.9986114042749594	3.2792407369992143
6	1.6443116595396725	3.237504059119313
7	1.4548363578484855	3.3418512954383077
8	1.2677056077705573	3.328000120589039
9	1.0707151124432412	3.162214872026591
>10	7.687985692965197	42.689615681510546
>50	0.06943992078814505	1.4976365923996542
>100	0.026733019524915618	1.7154321564896613
>500	0.001461959844870573	0.3150553383806282
>1k	2.0885140641008183E-4	0.11328833464067402
>5k	0.0	0.0
>10k+	4.1770281282016365E-4	2.325255062122679
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22013	1.4689052908750106	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11915	0.7950759342559283	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1653	0.11030302302350395	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6691596617907793E-4	0.0	0.0	0.008874955875454341	0.0
2	2.6691596617907793E-4	0.0	0.0	0.03196318694994458	0.0
3	2.6691596617907793E-4	0.0	0.0	0.06225814911126993	0.0
4	2.6691596617907793E-4	0.0	0.0	0.08955030665308064	0.0
5	2.6691596617907793E-4	0.0	0.0	0.1770987435598182	0.0
6	2.6691596617907793E-4	0.0	0.0	0.3393836509966976	0.0
7	2.6691596617907793E-4	0.0	0.0	0.4427468588995455	0.0
8	2.6691596617907793E-4	0.0	0.0	0.6278530814447361	0.0
9	2.6691596617907793E-4	0.0	0.0	0.9583617765659793	0.0
10	2.6691596617907793E-4	0.0	0.0	1.402509944287965	0.0
11	2.6691596617907793E-4	0.0	0.0	1.6542784293863801	0.0
12	2.6691596617907793E-4	0.0	0.0	1.887963357776163	0.0
13	2.6691596617907793E-4	0.0	0.0	2.0027372232331664	0.0
14	2.6691596617907793E-4	0.0	0.0	2.0631936895727274	0.0
15	2.6691596617907793E-4	0.0	0.0	2.112239498358133	0.0
16	2.6691596617907793E-4	0.0	0.0	2.1810370886407906	0.0
17	2.6691596617907793E-4	0.0	0.0	2.249434304974179	0.0
18	2.6691596617907793E-4	0.0	0.0	2.3773537817655024	0.0
19	2.6691596617907793E-4	0.0	0.0	2.43020314306896	0.0
20	2.6691596617907793E-4	0.0	0.0	2.493328769070312	0.0
21	2.6691596617907793E-4	0.0	0.0	2.5582560778433723	0.0
22	2.6691596617907793E-4	0.0	0.0	2.6279211450161117	0.0
23	2.6691596617907793E-4	0.0	0.0	2.709197056717641	0.0
24	2.6691596617907793E-4	0.0	0.0	2.7785284789326563	0.0
25	2.6691596617907793E-4	0.0	0.0	2.8404529830862026	0.0
26	2.6691596617907793E-4	0.0	0.0	2.901710197324301	0.0
27	2.6691596617907793E-4	0.0	0.0	2.977447602727614	0.0
28	2.6691596617907793E-4	0.0	0.0	3.041574163602138	0.0
29	2.6691596617907793E-4	0.0	0.0	3.1109055858171533	0.0
30	2.6691596617907793E-4	0.0	0.0	3.2268805731219627	0.0
31	2.6691596617907793E-4	0.0	0.0	3.30869031675585	0.0
32	2.6691596617907793E-4	0.0	0.0	3.403045110800154	0.0
33	2.6691596617907793E-4	0.0	0.0	3.492995791402503	0.0
34	2.6691596617907793E-4	0.0	0.0	3.580143854359972	0.0
35	2.6691596617907793E-4	0.0	0.0	3.6753661252943584	0.0
36	2.6691596617907793E-4	0.0	0.0	3.76311474917573	0.0
37	2.6691596617907793E-4	0.0	0.0	3.85586804742296	0.0
38	2.6691596617907793E-4	0.0	0.0	3.957095927596375	0.0
39	2.6691596617907793E-4	0.0	0.0	4.083814282539892	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	18245	0.0	43.618797	1
GTTTTTA	12255	0.0	42.63158	2
CGCATCG	80	0.0	42.1875	21
TATACGA	70	0.0	41.785713	2
CGGTCTA	190	0.0	41.44737	31
CACGACG	185	0.0	41.351353	26
TTAACGA	105	0.0	40.714287	2
TCACGAC	190	0.0	40.263157	25
TAATGCG	45	1.9290383E-8	40.0	1
CACGACC	205	0.0	39.512196	27
TTATTTT	14075	0.0	38.669624	6
AGCGATA	205	0.0	38.414635	8
TATTTTT	14145	0.0	38.398727	7
CTCACGA	200	0.0	38.250004	24
AGGGGAA	7770	0.0	38.19498	8
TTTTATT	14280	0.0	38.17752	4
TTTATTT	14385	0.0	37.88321	5
AGACTTA	815	0.0	37.822086	8
GAGCGAT	1195	0.0	37.656906	7
TAAACGG	30	1.1400871E-4	37.500004	2
>>END_MODULE