FastQCFastQC Report
Thu 26 May 2016
SRR1546869_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546869_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences653861
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112131.7148904736633628No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62100.949743141126325No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCG207.0310524E-445.010
TACGGGT302.1640608E-644.9999964
CGTTTTT79600.043.8976141
GTTTTTA53800.042.5325282
TTGTGCG603.6379788E-1241.2499961
CGGTCTA1750.041.14285731
ACGGTCT1750.041.14285730
GAGCGAT5100.040.147067
TAACGCC451.9261279E-840.012
TTATTTT59500.039.819336
TATTTTT59600.039.7147677
GCGCGAC3150.039.2857139
TTTTATT60750.039.1851844
CGACGGT1900.039.07894528
CGTTGAG750.039.03
TTTATTT60750.039.05
GTACGGG1000.038.253
CTATCTC3300.038.1818166
GACGGTC1950.038.07692329
CGTAGGA659.094947E-1238.076922
Produced by FastQC (version 0.11.5)