##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546869_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	653861
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.674082106135707	31.0	31.0	34.0	30.0	34.0
2	32.02290395053383	31.0	31.0	34.0	30.0	34.0
3	31.91313750170143	31.0	31.0	34.0	30.0	34.0
4	35.752929139373656	37.0	35.0	37.0	35.0	37.0
5	35.78447712893107	37.0	35.0	37.0	35.0	37.0
6	35.83671758982414	37.0	35.0	37.0	35.0	37.0
7	36.07456018939805	37.0	35.0	37.0	35.0	37.0
8	13.224056794945716	0.0	0.0	35.0	0.0	37.0
9	24.895379904903336	18.0	17.0	39.0	17.0	39.0
10	32.623514783723145	32.0	32.0	37.0	27.0	39.0
11	35.535525134546944	37.0	35.0	37.0	32.0	39.0
12	36.68276896771638	38.0	35.0	39.0	33.0	39.0
13	36.97079807482018	39.0	37.0	39.0	33.0	39.0
14	38.17639528890697	40.0	37.0	41.0	33.0	41.0
15	38.41882601959744	40.0	38.0	41.0	34.0	41.0
16	38.477665742413144	40.0	38.0	41.0	34.0	41.0
17	38.417992509111265	40.0	38.0	41.0	34.0	41.0
18	38.31210608982643	40.0	37.0	41.0	34.0	41.0
19	38.238512466716934	40.0	37.0	41.0	34.0	41.0
20	38.159243325416256	40.0	37.0	41.0	34.0	41.0
21	38.10057948096002	40.0	36.0	41.0	33.0	41.0
22	38.05055661677329	40.0	36.0	41.0	33.0	41.0
23	37.85637773165856	39.0	36.0	41.0	33.0	41.0
24	37.54335248623178	39.0	35.0	41.0	33.0	41.0
25	37.429747301031874	39.0	35.0	41.0	33.0	41.0
26	37.357903285254814	39.0	35.0	41.0	33.0	41.0
27	37.313799110208436	39.0	35.0	41.0	33.0	41.0
28	37.16319829443873	39.0	35.0	41.0	33.0	41.0
29	37.07714942472483	39.0	35.0	41.0	33.0	41.0
30	36.88296595147899	39.0	35.0	41.0	32.0	41.0
31	36.710403281431375	39.0	35.0	41.0	31.0	41.0
32	36.371063574674125	39.0	35.0	41.0	30.0	41.0
33	35.99729606139531	39.0	35.0	41.0	29.0	41.0
34	35.560837853916965	39.0	35.0	41.0	25.0	41.0
35	35.24692556980765	39.0	35.0	41.0	23.0	41.0
36	35.07567816401345	39.0	35.0	41.0	22.0	41.0
37	35.01890768833131	39.0	35.0	41.0	22.0	41.0
38	34.890695423033335	39.0	35.0	40.0	21.0	41.0
39	34.78093815046317	38.0	34.0	40.0	21.0	41.0
40	34.661680387727664	38.0	34.0	40.0	20.0	41.0
41	34.54547679093875	38.0	34.0	40.0	18.0	41.0
42	34.45666739566972	38.0	34.0	40.0	18.0	41.0
43	34.34013804157153	38.0	34.0	40.0	18.0	41.0
44	34.09886046116835	38.0	34.0	40.0	18.0	41.0
45	34.04015226477799	38.0	33.0	40.0	18.0	41.0
46	34.0167344435591	38.0	33.0	40.0	18.0	41.0
47	33.96750226730146	38.0	33.0	40.0	18.0	41.0
48	33.876871383979164	37.0	33.0	40.0	18.0	41.0
49	33.784848767551516	37.0	33.0	40.0	18.0	41.0
50	33.67976832996615	37.0	33.0	40.0	18.0	41.0
51	33.33731022342669	36.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	5.0
14	6.0
15	12.0
16	40.0
17	101.0
18	231.0
19	494.0
20	1021.0
21	1739.0
22	2859.0
23	4636.0
24	7690.0
25	11986.0
26	15846.0
27	16587.0
28	15182.0
29	14265.0
30	15341.0
31	18011.0
32	22860.0
33	30821.0
34	44592.0
35	65562.0
36	69066.0
37	109508.0
38	129229.0
39	56160.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.96890776480016	20.853514737841834	25.29819028814993	23.879387209208073
2	39.642064597827364	22.513041762698798	26.305590943640926	11.539302695832907
3	26.48376336866704	22.97338425139288	38.516748972640976	12.026103407299104
4	23.85874061918359	25.213768675605365	37.55966482172817	13.367825883482881
5	21.04591036932926	29.053422669344098	36.58606339879577	13.314603562530875
6	19.47585190124507	36.831834288939085	34.04072119303644	9.651592616779407
7	78.08937985290451	3.614223818212128	16.38712203358206	1.9092742953012949
8	29.45687233219293	63.59700303275467	5.880760589788961	1.065364045263443
9	74.0590125424211	4.50997994986702	17.909616875757997	3.5213906319538864
10	39.118252962020975	24.271672419673294	23.69999128255088	12.910083335754846
11	32.25960869359084	21.207565522335788	31.004296020102135	15.52852976397124
12	28.848486146138093	19.38378340350625	34.73674068341742	17.030989766938234
13	23.52151298211699	22.9198560550331	36.106909572523826	17.451721390326078
14	18.19209281483373	26.245180550606324	37.63659248678236	17.926134147777585
15	17.786960837242166	21.43116044541577	43.804417146763605	16.977461570578456
16	21.677543086374627	19.669012221251915	41.21594650850869	17.437498183864765
17	21.040557549693283	20.282292413831073	36.079227848120624	22.597922188355017
18	22.43443178290187	21.823598593584876	37.05328808416468	18.68868153934858
19	24.97472704443299	23.879081333800304	32.993709672239206	18.1524819495275
20	25.4696334542051	23.96487938567983	32.26587913945013	18.29960802066494
21	23.499489952757543	23.942703418616496	35.02074599953201	17.53706062909395
22	21.548004851183965	21.49921772364463	35.72624762755387	21.226529797617534
23	21.23172967954963	23.41017433368866	34.81198603372888	20.546109953032833
24	22.02884099219865	22.197990092695544	36.709484125831025	19.063684789274784
25	22.02501754960152	22.56152301483037	34.99964059639587	20.41381883917224
26	18.89178281010796	25.243132714751297	33.96991103613765	21.89517343900309
27	19.235739706145498	23.423938727038315	35.98945341594009	21.350868150876103
28	18.725386588280994	24.014890014850252	37.43058539964916	19.829137997219593
29	20.52745155315885	23.754131229726198	36.23247142741347	19.485945789701482
30	21.451195284624713	23.107357679996205	36.12296803143175	19.318479003947324
31	24.54267803095765	22.40369130442097	32.617482920681915	20.43614774393946
32	25.717698409906692	23.52885399190348	31.846676893101133	18.906770705088697
33	24.029419096719334	23.239801731560682	31.85570021763035	20.87507895408963
34	20.922642579997888	24.834483169970376	33.59643716325029	20.64643708678144
35	20.978923655027597	24.85145925510162	33.435852574170966	20.73376451569982
36	23.41858590740234	26.16825288555213	30.53768308554876	19.87547812149677
37	22.333187022929952	27.325226615442734	30.588305465534727	19.753280896092594
38	21.928666796153923	28.050151331857993	29.06917525284426	20.952006619143823
39	21.858621327774557	26.152347364348078	29.726348566438432	22.26268274143893
40	22.545158680514664	24.094876433982147	30.964379279388126	22.395585606115063
41	20.262410512326014	24.423539559631173	31.106305468593476	24.20774445944933
42	21.53209932997992	26.783521268281792	28.98597714193078	22.698402259807512
43	21.26124665639945	25.51918527026386	29.731242572962756	23.488325500373932
44	21.551216542965555	25.934717011719616	30.30812359201726	22.205942853297568
45	20.85427942636126	27.05758563364385	29.715795864870366	22.37233907512453
46	22.374633140682807	26.879413208617738	29.700196219074083	21.045757431625375
47	21.22423573205926	26.152806177459738	30.546247596966325	22.076710493514675
48	21.20282445351535	24.69928623973597	31.862429476601296	22.235459830147384
49	21.873303347347527	23.7223201873181	31.7250914185125	22.679285046821878
50	20.405254327754676	25.10548878125473	32.210056877532075	22.279200013458517
51	19.75022214201489	26.537750378138476	31.55166006230682	22.160367417539813
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	531.0
1	1050.0
2	1569.0
3	14592.0
4	27615.0
5	18209.0
6	8803.0
7	8507.5
8	8212.0
9	8129.5
10	8047.0
11	7872.5
12	7698.0
13	7339.0
14	6980.0
15	6478.0
16	5976.0
17	5514.0
18	5052.0
19	4731.0
20	4410.0
21	4294.5
22	4179.0
23	4370.0
24	4561.0
25	4952.0
26	5923.5
27	6504.0
28	7326.5
29	8149.0
30	8949.0
31	9749.0
32	10697.0
33	11645.0
34	12887.5
35	14130.0
36	16119.0
37	18108.0
38	21165.0
39	24222.0
40	28093.0
41	31964.0
42	34939.0
43	37914.0
44	40248.5
45	42583.0
46	45242.0
47	47901.0
48	46956.5
49	46012.0
50	43323.5
51	40635.0
52	38416.5
53	36198.0
54	34669.5
55	33141.0
56	32466.5
57	31792.0
58	30829.5
59	29867.0
60	27597.0
61	25327.0
62	22515.5
63	19704.0
64	16740.0
65	13776.0
66	11465.5
67	9155.0
68	7801.0
69	6447.0
70	5381.5
71	4316.0
72	3504.5
73	2693.0
74	2074.5
75	1102.5
76	749.0
77	553.5
78	358.0
79	284.0
80	210.0
81	158.0
82	106.0
83	71.5
84	37.0
85	27.0
86	17.0
87	12.0
88	7.0
89	7.5
90	8.0
91	5.5
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	653861.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.149047174420296
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.33805250948627	32.220740615777785
2	11.160762793405437	10.524386628975755
3	6.39296682100863	9.042668826847192
4	4.238424284656976	7.993506661700015
5	2.8881166640967457	6.8085974420363415
6	1.990958041117983	5.632306476177791
7	1.3692872887972618	4.519241368238531
8	1.0217011117768655	3.853778713386008
9	0.7132187768030654	3.0264827177853064
>10	1.846233778109865	11.600456785494291
>50	0.024036838423491195	0.774635312476202
>100	0.01559144862465482	1.3348589349198652
>500	0.0	0.0
>1k	0.0	0.0
>5k	3.2482184634597864E-4	0.9510640185678934
>10k+	3.2482184634597864E-4	1.717275497617035
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11213	1.7148904736633628	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6210	0.949743141126325	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010552701568070278	0.0
2	0.0	0.0	0.0	0.035175671893567594	0.0
3	0.0	0.0	0.0	0.07325716016095164	0.0
4	0.0	0.0	0.0	0.11562090413711783	0.0
5	0.0	0.0	0.0	0.24102982132288056	0.0
6	0.0	0.0	0.0	0.47395394433985205	0.0
7	0.0	0.0	0.0	0.6330091563803316	0.0
8	0.0	0.0	0.0	0.8882621841645243	0.0
9	0.0	0.0	0.0	1.3487576105624897	0.0
10	0.0	0.0	0.0	1.9554614818745881	0.0
11	0.0	0.0	0.0	2.27877178788764	0.0
12	0.0	0.0	0.0	2.5682828613420896	0.0
13	0.0	0.0	0.0	2.724585194712638	0.0
14	0.0	0.0	0.0	2.823382951422397	0.0
15	0.0	0.0	0.0	2.8961812984716935	0.0
16	0.0	0.0	0.0	2.981826412647336	0.0
17	0.0	0.0	0.0	3.0760360382405434	0.0
18	0.0	0.0	0.0	3.243502823994702	0.0
19	0.0	0.0	0.0	3.3254774332771033	0.0
20	0.0	0.0	0.0	3.413416613011022	0.0
21	0.0	0.0	0.0	3.511449681201356	0.0
22	0.0	0.0	0.0	3.6260000214112784	0.0
23	0.0	0.0	0.0	3.7518677517086965	0.0
24	0.0	0.0	0.0	3.8583123936127097	0.0
25	0.0	0.0	0.0	3.9462515733466286	0.0
26	0.0	0.0	0.0	4.026085054774638	0.0
27	0.0	0.0	0.0	4.11891824103288	0.0
28	0.0	0.0	0.0	4.208539735509535	0.0
29	0.0	0.0	0.0	4.310396246296996	0.0
30	0.0	0.0	0.0	4.450028369944071	0.0
31	1.529377038850765E-4	0.0	0.0	4.557849451183049	0.0
32	1.529377038850765E-4	0.0	0.0	4.677905548732834	0.0
33	1.529377038850765E-4	0.0	0.0	4.80010277413701	0.0
34	1.529377038850765E-4	0.0	0.0	4.9149589897547035	0.0
35	1.529377038850765E-4	0.0	0.0	5.044497224945363	0.0
36	1.529377038850765E-4	0.0	0.0	5.156753499597009	0.0
37	1.529377038850765E-4	0.0	0.0	5.289503426569255	0.0
38	1.529377038850765E-4	0.0	0.0	5.443817569789298	0.0
39	1.529377038850765E-4	0.0	0.0	5.659612669971141	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATTCG	20	7.0310524E-4	45.0	10
TACGGGT	30	2.1640608E-6	44.999996	4
CGTTTTT	7960	0.0	43.897614	1
GTTTTTA	5380	0.0	42.532528	2
TTGTGCG	60	3.6379788E-12	41.249996	1
CGGTCTA	175	0.0	41.142857	31
ACGGTCT	175	0.0	41.142857	30
GAGCGAT	510	0.0	40.14706	7
TAACGCC	45	1.9261279E-8	40.0	12
TTATTTT	5950	0.0	39.81933	6
TATTTTT	5960	0.0	39.714767	7
GCGCGAC	315	0.0	39.285713	9
TTTTATT	6075	0.0	39.185184	4
CGACGGT	190	0.0	39.078945	28
CGTTGAG	75	0.0	39.0	3
TTTATTT	6075	0.0	39.0	5
GTACGGG	100	0.0	38.25	3
CTATCTC	330	0.0	38.181816	6
GACGGTC	195	0.0	38.076923	29
CGTAGGA	65	9.094947E-12	38.07692	2
>>END_MODULE