FastQCFastQC Report
Thu 26 May 2016
SRR1546868_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546868_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1978235
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247761.2524295647382642No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138160.6984003417187543No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAT207.0345215E-445.01
CGTTTTT224600.043.9180761
GTTTTTA153000.043.1176452
TATCGCG750.042.01
CGACGGT3400.041.69117728
TCACGAC3350.041.6417925
CACGACG3450.040.43478426
ATGGTCG1950.040.38461744
ACGGTCT3400.040.3676530
TTATTTT169950.039.9029126
CGGTCTA3450.039.78260831
AGACCGA4950.039.5454528
TATTTTT171750.039.524027
TTTTATT173400.039.2387544
GCGCGAC12700.039.153549
TACGAGT2300.039.1304364
GAGCGAT19100.039.1099477
TTTATTT175150.038.8081635
TAGCCGT3500.038.57142644
TTTTTAT176900.038.5132833
Produced by FastQC (version 0.11.5)