##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546868_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1978235
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.70819745884589	31.0	31.0	34.0	30.0	34.0
2	32.06747984946177	33.0	31.0	34.0	30.0	34.0
3	31.99065126236266	33.0	31.0	34.0	30.0	34.0
4	35.81362022206664	37.0	35.0	37.0	35.0	37.0
5	35.849794892922226	37.0	35.0	37.0	35.0	37.0
6	35.90064577767556	37.0	35.0	37.0	35.0	37.0
7	36.126909593652925	37.0	35.0	37.0	35.0	37.0
8	13.29074503281966	0.0	0.0	36.0	0.0	37.0
9	24.966130919734006	18.0	17.0	39.0	17.0	39.0
10	32.50060634858851	32.0	32.0	37.0	27.0	39.0
11	35.5191450965128	37.0	34.0	37.0	32.0	39.0
12	36.771839038334676	38.0	35.0	39.0	33.0	39.0
13	37.11986543560295	39.0	37.0	39.0	34.0	39.0
14	38.36349220390904	40.0	38.0	41.0	34.0	41.0
15	38.583541389167614	40.0	38.0	41.0	34.0	41.0
16	38.61598798929349	40.0	38.0	41.0	34.0	41.0
17	38.58416922155356	40.0	38.0	41.0	34.0	41.0
18	38.51169350456341	40.0	38.0	41.0	34.0	41.0
19	38.44426117220654	40.0	37.0	41.0	34.0	41.0
20	38.37230005535237	40.0	37.0	41.0	34.0	41.0
21	38.320528653067	40.0	37.0	41.0	34.0	41.0
22	38.26479513303526	40.0	37.0	41.0	34.0	41.0
23	38.058624480913544	40.0	37.0	41.0	34.0	41.0
24	37.763116111078816	40.0	35.0	41.0	34.0	41.0
25	37.65583916976497	39.0	35.0	41.0	33.0	41.0
26	37.594584061044316	39.0	35.0	41.0	33.0	41.0
27	37.547295948155806	39.0	35.0	41.0	33.0	41.0
28	37.441654302951875	39.0	35.0	41.0	33.0	41.0
29	37.35199306452469	39.0	35.0	41.0	33.0	41.0
30	37.190392445791325	39.0	35.0	41.0	33.0	41.0
31	37.03855760311591	39.0	35.0	41.0	32.0	41.0
32	36.73251256802149	40.0	35.0	41.0	31.0	41.0
33	36.363780845046215	40.0	35.0	41.0	30.0	41.0
34	35.9643146542246	40.0	35.0	41.0	27.0	41.0
35	35.65697806377908	39.0	35.0	41.0	24.0	41.0
36	35.47530247923022	39.0	35.0	41.0	23.0	41.0
37	35.388046920613576	39.0	35.0	41.0	23.0	41.0
38	35.25976286942653	39.0	35.0	41.0	23.0	41.0
39	35.1401744484351	39.0	35.0	41.0	22.0	41.0
40	35.02022257214133	39.0	35.0	41.0	21.0	41.0
41	34.91697700222673	39.0	35.0	40.0	21.0	41.0
42	34.82479937924463	39.0	35.0	40.0	20.0	41.0
43	34.70876816960573	38.0	34.0	40.0	20.0	41.0
44	34.5085063200277	38.0	34.0	40.0	18.0	41.0
45	34.409359353160774	38.0	34.0	40.0	19.0	41.0
46	34.36706205278948	38.0	34.0	40.0	19.0	41.0
47	34.33106127431776	38.0	34.0	40.0	20.0	41.0
48	34.22773381322239	38.0	34.0	40.0	19.0	41.0
49	34.12231105000164	38.0	34.0	40.0	18.0	41.0
50	34.024286295612	37.0	33.0	40.0	18.0	41.0
51	33.66617818408834	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	3.0
12	4.0
13	8.0
14	14.0
15	42.0
16	111.0
17	287.0
18	632.0
19	1389.0
20	2749.0
21	4808.0
22	8086.0
23	13165.0
24	21921.0
25	34075.0
26	44679.0
27	46492.0
28	42105.0
29	39618.0
30	41793.0
31	49442.0
32	62902.0
33	85177.0
34	124776.0
35	185210.0
36	209860.0
37	352157.0
38	417310.0
39	189377.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.31084021867978	20.897719431715643	26.05408356438947	21.737356785215102
2	38.83052316837989	22.17982191195687	27.24590354533208	11.74375137433116
3	27.64686703045897	22.917600790603746	37.350921402158995	12.08461077677829
4	23.944425207318645	25.364580042310443	36.5680518239744	14.122942926396508
5	21.840175712187886	29.43184202079126	35.30621993848052	13.421762328540341
6	19.123764365709835	37.910460587341746	32.881179435203606	10.084595611744813
7	80.10281892697279	3.550184887033138	14.600995331697195	1.746000854296886
8	30.295793977965207	63.408644574582894	5.314383781502198	0.9811776659496976
9	76.28385909661895	4.04774963540732	16.431263222013563	3.2371280459601617
10	35.38624076512649	21.729976468923056	28.215808536397347	14.667974229553113
11	30.937022143476383	23.147704898558562	29.38462821656679	16.530644741398266
12	27.173263034978152	21.624175085366502	32.863587996370505	18.338973883284847
13	22.96643219839908	24.59930190295895	34.15969285752198	18.274573041119986
14	19.81675584548853	27.935305967187922	34.81876521242421	17.429172974899345
15	19.75781441537532	22.57315233023377	39.9291287435517	17.739904510839207
16	21.185551767105526	20.539319140547	39.15262847942737	19.122500612920103
17	20.839839553945815	20.45439495307686	34.963692382350935	23.74207311062639
18	22.386420218022632	22.350024137678286	35.596680879673045	19.666874764626044
19	25.241389420367145	23.762899756601215	32.06337972991075	18.932331093120887
20	25.945350274360727	23.684395433302917	30.939347448609496	19.43090684372686
21	23.69713406142344	24.348168948582956	33.77414715642985	18.18054983356376
22	22.569411621976155	20.734745871951514	33.91806332412479	22.777779181947544
23	21.065141401299645	24.921255563671657	32.55199710853361	21.461605926495082
24	20.70138279830253	24.504823744398415	35.6896425348859	19.10415092241316
25	21.5613918467725	23.680351424375772	34.327418127775516	20.43083860107621
26	19.85997619089744	26.129201030211274	32.530563861219726	21.48025891767156
27	20.438926618930513	24.161537936594996	34.501108311196596	20.8984271332779
28	19.713330317176673	24.538540668828528	36.5224050732092	19.2257239407856
29	20.427957244715618	24.351404155724673	35.63418906247235	19.586449537087354
30	21.70869487194393	23.25972394584061	35.86661847556029	19.164962706655174
31	24.820054240269734	23.105546105493026	32.03411121530051	20.04028843893673
32	24.471056269856717	23.76527561184591	32.479103847621744	19.284564270675627
33	23.210488137152563	23.94745821401401	31.551610400179957	21.29044324865347
34	21.35585509305012	26.22827924892644	32.169534964248435	20.246330693775004
35	21.334320745513047	25.001680791210347	32.338978938296	21.3250195249806
36	24.75059838694594	26.786807431877406	28.92310569775583	19.53948848342083
37	22.575831486148008	26.889727459073363	29.355208051621773	21.179233003156856
38	22.898290647976605	28.822612075916158	27.127009682873876	21.15208759323336
39	22.351338440579607	26.104128174862947	27.769552151286376	23.774981233271074
40	22.875290347203443	25.24214767204099	29.77861578629435	22.103946194461223
41	21.64045222129828	25.29482088831711	29.527381731695172	23.537345158689437
42	22.973913614914306	26.946141383607102	28.037922693714346	22.042022307764245
43	22.46462124065139	26.234294712205575	28.61631707051993	22.6847669766231
44	22.61475507207182	26.87092281756212	29.41839569110849	21.095926419257573
45	21.658448061024092	27.998443056563048	28.89949879564359	21.443610086769265
46	23.24622706604625	26.370628363162112	29.323007630539344	21.060136940252296
47	22.864573723546496	26.78069086837509	28.93271021895781	21.422025189120607
48	22.60277469562514	26.57596291643814	29.45251701643131	21.36874537150541
49	22.768225210857153	25.329751015425366	30.040414814215705	21.861608959501776
50	21.541930053810592	26.409450848862747	30.16117903080271	21.887440066523947
51	21.659256862809524	27.68392026225398	29.298187525748965	21.358635349187534
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1565.0
1	2581.0
2	3597.0
3	33582.0
4	63567.0
5	43566.5
6	23566.0
7	23361.5
8	23157.0
9	23123.5
10	23090.0
11	22484.5
12	21879.0
13	21083.5
14	20288.0
15	18973.5
16	17659.0
17	16449.0
18	15239.0
19	14240.5
20	13242.0
21	12685.0
22	12128.0
23	12433.5
24	12739.0
25	13568.5
26	15439.0
27	16480.0
28	18430.5
29	20381.0
30	23412.0
31	26443.0
32	29471.5
33	32500.0
34	37320.5
35	42141.0
36	48503.0
37	54865.0
38	65407.0
39	75949.0
40	90279.5
41	104610.0
42	116680.5
43	128751.0
44	136216.0
45	143681.0
46	147229.5
47	150778.0
48	147022.0
49	143266.0
50	139870.5
51	136475.0
52	128354.0
53	120233.0
54	113802.0
55	107371.0
56	101441.5
57	95512.0
58	88918.5
59	82325.0
60	74007.0
61	65689.0
62	58489.0
63	51289.0
64	43602.5
65	35916.0
66	30202.0
67	24488.0
68	21493.0
69	18498.0
70	16293.0
71	14088.0
72	11835.5
73	9583.0
74	7586.0
75	4203.0
76	2817.0
77	2043.5
78	1270.0
79	972.5
80	675.0
81	467.0
82	259.0
83	180.0
84	101.0
85	75.5
86	50.0
87	40.5
88	31.0
89	19.0
90	7.0
91	6.0
92	5.0
93	4.0
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1978235.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.990295067800037
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.79830699140416	21.53252412040701
2	6.764813654052411	4.057575151274287
3	3.4721891354809813	3.1239593011285245
4	2.379835004345403	2.854878159719913
5	1.8068263994677332	2.7093628428164025
6	1.5183719686878898	2.732185401817575
7	1.3301544762507613	2.792420765996074
8	1.1198578801964576	2.6867894608874265
9	0.9808856397399148	2.6475344787211066
>10	8.660828925373508	47.94462468892638
>50	0.1333034186139826	2.49317913976118
>100	0.031828513584647194	1.8705611486858147
>500	0.0020984946015848672	0.409231406128901
>1k	3.497491002641445E-4	0.12120130457053571
>5k	0.0	0.0
>10k+	3.497491002641445E-4	2.0239726291588553
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24776	1.2524295647382642	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13816	0.6984003417187543	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0110022317874266E-4	0.0	0.0	0.006369314060260788	0.0
2	1.0110022317874266E-4	0.0	0.0	0.022848650438395842	0.0
3	1.0110022317874266E-4	0.0	0.0	0.04438299797546803	0.0
4	1.0110022317874266E-4	0.0	0.0	0.0685965014267769	0.0
5	1.0110022317874266E-4	0.0	0.0	0.13198634135984855	0.0
6	1.0110022317874266E-4	0.0	0.0	0.2816146716643877	0.0
7	1.0110022317874266E-4	0.0	0.0	0.38680945388186944	0.0
8	1.0110022317874266E-4	0.0	0.0	0.6064496887376879	0.0
9	1.0110022317874266E-4	0.0	0.0	0.974707251666258	0.0
10	1.0110022317874266E-4	0.0	0.0	1.4760127082980536	0.0
11	1.0110022317874266E-4	0.0	0.0	1.710615776184326	0.0
12	1.0110022317874266E-4	0.0	0.0	1.9178712337007484	0.0
13	1.0110022317874266E-4	0.0	0.0	2.02872762841624	0.0
14	1.0110022317874266E-4	0.0	0.0	2.08660750618607	0.0
15	1.0110022317874266E-4	0.0	0.0	2.134933412865509	0.0
16	1.0110022317874266E-4	0.0	0.0	2.2127300346015515	0.0
17	1.0110022317874266E-4	0.0	0.0	2.294368464818386	0.0
18	1.0110022317874266E-4	0.0	0.0	2.432976870796442	0.0
19	1.0110022317874266E-4	0.0	0.0	2.4959117597252094	0.0
20	1.0110022317874266E-4	0.0	0.0	2.5684006197443683	0.0
21	1.0110022317874266E-4	0.0	0.0	2.6420015822184926	0.0
22	1.0110022317874266E-4	0.0	0.0	2.7251565157830084	0.0
23	1.0110022317874266E-4	0.0	0.0	2.8198874249014905	0.0
24	1.0110022317874266E-4	0.0	0.0	2.9015258551183254	0.0
25	1.0110022317874266E-4	0.0	0.0	2.973003712905696	0.0
26	1.0110022317874266E-4	0.0	0.0	3.0394265595341303	0.0
27	1.0110022317874266E-4	0.0	0.0	3.1161110788152064	0.0
28	1.0110022317874266E-4	0.001364853012913026	0.0	3.194716502336679	0.0
29	1.0110022317874266E-4	0.001364853012913026	0.0	3.2845946007425812	0.0
30	1.0110022317874266E-4	0.001364853012913026	0.0	3.409150075698792	0.0
31	1.0110022317874266E-4	0.001364853012913026	0.0	3.5077733434096556	0.0
32	1.0110022317874266E-4	0.001364853012913026	0.0	3.613372526519852	0.0
33	1.0110022317874266E-4	0.001364853012913026	0.0	3.7196794111923004	0.0
34	1.0110022317874266E-4	0.001364853012913026	0.0	3.8217906366028305	0.0
35	1.0110022317874266E-4	0.001364853012913026	0.0	3.9281986214984568	0.0
36	1.0110022317874266E-4	0.001364853012913026	0.0	4.030562597466934	0.0
37	1.0110022317874266E-4	0.001364853012913026	0.0	4.130348517744353	0.0
38	1.0110022317874266E-4	0.001364853012913026	0.0	4.259706253301554	0.0
39	1.0110022317874266E-4	0.001364853012913026	0.0	4.426117220653764	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTAAT	20	7.0345215E-4	45.0	1
CGTTTTT	22460	0.0	43.918076	1
GTTTTTA	15300	0.0	43.117645	2
TATCGCG	75	0.0	42.0	1
CGACGGT	340	0.0	41.691177	28
TCACGAC	335	0.0	41.64179	25
CACGACG	345	0.0	40.434784	26
ATGGTCG	195	0.0	40.384617	44
ACGGTCT	340	0.0	40.36765	30
TTATTTT	16995	0.0	39.902912	6
CGGTCTA	345	0.0	39.782608	31
AGACCGA	495	0.0	39.545452	8
TATTTTT	17175	0.0	39.52402	7
TTTTATT	17340	0.0	39.238754	4
GCGCGAC	1270	0.0	39.15354	9
TACGAGT	230	0.0	39.130436	4
GAGCGAT	1910	0.0	39.109947	7
TTTATTT	17515	0.0	38.808163	5
TAGCCGT	350	0.0	38.571426	44
TTTTTAT	17690	0.0	38.513283	3
>>END_MODULE