FastQCFastQC Report
Thu 26 May 2016
SRR1546867_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546867_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1212239
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229371.892118633371802No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129431.0676937468601488No Hit
GAATCTGACTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTC15600.12868749479269354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGA406.8157533E-945.0000042
TTGTCGA207.033439E-445.0000042
GATTACG551.8189894E-1245.01
GTTAACG253.8909337E-544.9999961
CGTTTTT197400.043.7803961
GTTTTTA131400.043.339042
TAACGCC1750.042.42857412
AGCGATA2150.041.8604628
TATACGA603.6379788E-1241.2499962
TAGCCGT1600.040.78125444
TATTTTT143950.040.7485247
TTATTTT145250.040.352846
GAGCGAT10400.040.0240367
TTTTATT147100.039.921824
CAACCCG2100.039.64285323
TTTATTT147950.039.6316345
GTAGCAT10550.039.45497529
CGCATCG1550.039.1935521
ATCGCCA1550.039.1935524
ATAACGC1900.039.0789511
Produced by FastQC (version 0.11.5)