##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546867_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1212239
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.639108294651468	31.0	31.0	34.0	30.0	34.0
2	31.976262106729777	31.0	31.0	34.0	30.0	34.0
3	31.806948134815	31.0	31.0	34.0	30.0	34.0
4	35.69116898565382	37.0	35.0	37.0	33.0	37.0
5	35.74180174041587	37.0	35.0	37.0	35.0	37.0
6	35.79863294284378	37.0	35.0	37.0	35.0	37.0
7	36.00722629778451	37.0	35.0	37.0	35.0	37.0
8	13.257109365397417	0.0	0.0	35.0	0.0	37.0
9	24.86306248190332	18.0	17.0	38.0	17.0	39.0
10	32.59690127111898	32.0	32.0	37.0	27.0	39.0
11	35.54277910544043	37.0	35.0	37.0	32.0	39.0
12	36.69763140766796	38.0	35.0	39.0	33.0	39.0
13	37.0000709431061	39.0	37.0	39.0	33.0	39.0
14	38.231229155306835	40.0	37.0	41.0	33.0	41.0
15	38.44122817365223	40.0	38.0	41.0	34.0	41.0
16	38.478767800738964	40.0	38.0	41.0	34.0	41.0
17	38.385062681533924	40.0	38.0	41.0	33.0	41.0
18	38.265462503681206	40.0	37.0	41.0	33.0	41.0
19	38.172541883242495	40.0	37.0	41.0	34.0	41.0
20	38.03316425226379	40.0	36.0	41.0	33.0	41.0
21	38.01316242094175	40.0	36.0	41.0	33.0	41.0
22	37.959091400293175	40.0	36.0	41.0	33.0	41.0
23	37.70362032569485	39.0	36.0	41.0	33.0	41.0
24	37.292231152437765	39.0	35.0	41.0	33.0	41.0
25	37.171476911731105	39.0	35.0	41.0	33.0	41.0
26	37.091101672195	39.0	35.0	41.0	33.0	41.0
27	37.032492767515315	39.0	35.0	41.0	33.0	41.0
28	36.838218371129784	39.0	35.0	41.0	32.0	41.0
29	36.686436420540836	38.0	35.0	41.0	32.0	41.0
30	36.47330518156898	38.0	35.0	41.0	31.0	41.0
31	36.2302879217712	38.0	35.0	41.0	30.0	41.0
32	35.790030678768794	38.0	35.0	41.0	27.0	41.0
33	35.2594711108948	38.0	35.0	41.0	23.0	41.0
34	34.680936679978124	38.0	34.0	41.0	20.0	41.0
35	34.263542915217215	38.0	34.0	40.0	17.0	41.0
36	34.0094593557871	38.0	33.0	40.0	15.0	41.0
37	33.90574630910241	38.0	33.0	40.0	15.0	41.0
38	33.74410161692538	38.0	33.0	40.0	14.0	41.0
39	33.6031764363298	38.0	33.0	40.0	12.0	41.0
40	33.450563791463566	38.0	33.0	40.0	12.0	41.0
41	33.32031554833659	38.0	33.0	40.0	11.0	41.0
42	33.21555815313647	37.0	33.0	40.0	10.0	41.0
43	33.09778929732503	37.0	33.0	40.0	10.0	41.0
44	32.88529077186924	37.0	32.0	40.0	10.0	41.0
45	32.812789392190815	37.0	32.0	40.0	10.0	41.0
46	32.782994112547115	37.0	32.0	40.0	10.0	41.0
47	32.736859645663934	36.0	32.0	40.0	10.0	41.0
48	32.64640223586273	36.0	32.0	40.0	10.0	41.0
49	32.53134489156016	36.0	31.0	40.0	10.0	41.0
50	32.42559346795475	35.0	31.0	40.0	10.0	41.0
51	32.08735488628893	35.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	5.0
13	9.0
14	12.0
15	47.0
16	86.0
17	302.0
18	621.0
19	1405.0
20	2691.0
21	4738.0
22	7493.0
23	11772.0
24	18613.0
25	29045.0
26	37475.0
27	39286.0
28	34879.0
29	31510.0
30	31156.0
31	35678.0
32	43339.0
33	56698.0
34	81304.0
35	117059.0
36	127728.0
37	196990.0
38	211554.0
39	90729.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.195828545361103	19.111000388537246	23.096105635934826	26.597065430166822
2	43.55123040918499	21.375570328953284	23.565237548041267	11.50796171382046
3	26.62470024475372	22.03294894818596	39.44766667299105	11.894684134069273
4	23.427228459074488	23.17257570495587	39.60819607354655	13.791999762423087
5	21.173794936477048	26.776155527086654	38.106841967631794	13.943207568804503
6	18.555664353316466	34.70825472534707	36.85428368498292	9.881797236353556
7	72.62074557904835	4.104141180080826	21.146902549744727	2.12821069112609
8	27.196452184758947	63.810106752876294	7.6713420373375225	1.322099025027243
9	67.82763134992356	5.080351316860784	22.37454825327349	4.717469079942157
10	36.42202568965361	21.8231718332771	28.108483558110244	13.64631891895905
11	30.290644006668654	21.36138170773255	33.24022738090426	15.107746904694535
12	26.552189790957065	18.44034055990609	37.94639505906014	17.061074590076707
13	23.572991794522366	20.346647814498624	38.555020915842505	17.525339475136505
14	18.545517839303965	24.573454574551718	39.81632334877858	17.06470423736573
15	18.10030860251155	19.867369388379686	44.118692766030456	17.913629243078304
16	21.764355048798134	18.076220943229842	41.80165792389124	18.35776608408078
17	20.488946486625164	18.391670289439624	39.22840297994043	21.89098024399479
18	22.09968496311371	20.142892614410197	38.62258185060867	19.13484057186743
19	25.2762037848972	22.43913947662136	34.452364591470825	17.832292147010616
20	25.574824766403324	23.045455557856165	33.77749767166376	17.602222004076754
21	23.635025766371154	23.148240569722635	36.645991425783194	16.57074223812301
22	21.404854983217007	19.687206895669913	37.46975637642412	21.43818174468896
23	21.103594258227957	22.602061144708262	36.22066275709658	20.0736818399672
24	22.06602823370639	22.029154316929255	37.70230127887322	18.20251617049113
25	22.683975684662844	21.19648023203345	35.986055555051436	20.133488528252265
26	19.005163173268638	22.503895683936914	36.63551494383533	21.855426198959115
27	20.602042996471816	20.220435079221176	37.577078447401874	21.60044347690513
28	19.89805640636871	22.59694664171009	38.238334189875104	19.2666627620461
29	21.16835046554351	22.585562747939967	37.745692062373834	18.500394724142684
30	22.261121775491468	20.729823079442255	38.20698723601534	18.802067909050937
31	24.655204130538618	20.28923339374496	35.108258354994355	19.947304120722066
32	25.56220349287558	21.24308820290388	34.53980609434279	18.654902209877754
33	25.245929226827386	21.13980823913436	32.7790146992466	20.835247834791655
34	21.52232356820726	24.81367123149808	34.2547962901705	19.40920891012416
35	22.41678414900032	24.487910387308112	33.44167280544513	19.653632658246433
36	24.00417739406173	27.17137462167114	29.568014228217372	19.256433756049756
37	23.21448163274734	27.350629702558653	29.332829582285342	20.102059082408665
38	24.053590092382773	28.28254164401574	27.406064315700124	20.257803947901362
39	23.716940306325732	25.490187990981976	27.706830088786123	23.08604161390617
40	23.701184337412013	23.63337592669432	30.93911349164645	21.726326244247215
41	21.725418832424957	24.200095855685223	30.383694964441833	23.690790347447987
42	22.725139184599737	26.522657660741817	28.42929488326972	22.322908271388727
43	22.062233602449684	24.631611423159956	29.54186426933963	23.764290705050737
44	22.334044689207328	24.82653998097735	30.969718017651637	21.86969731216369
45	21.932226235915525	25.329246130507265	30.805888937742477	21.932638695834733
46	23.288311958285455	25.354736153514285	30.61096037992508	20.745991508275182
47	21.541214232506956	25.955772747783236	31.1035200154425	21.399493004267313
48	21.916470267001802	25.0146217041359	31.667022757063584	21.40188527179871
49	22.915448191322007	23.81370340337178	31.84240071471055	21.428447690595668
50	21.3405937278045	23.81287848353336	32.20041592458253	22.646111864079607
51	21.202832114789246	25.600397281394184	31.74110055855322	21.45567004526335
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	954.0
1	2207.0
2	3460.0
3	32248.0
4	61036.0
5	41271.0
6	21506.0
7	20884.5
8	20263.0
9	20017.5
10	19772.0
11	19293.0
12	18814.0
13	17825.0
14	16836.0
15	15615.0
16	14394.0
17	13345.0
18	12296.0
19	11370.0
20	10444.0
21	10040.5
22	9637.0
23	9430.0
24	9223.0
25	9311.0
26	9952.0
27	10505.0
28	11415.0
29	12325.0
30	13360.0
31	14395.0
32	15622.0
33	16849.0
34	18477.0
35	20105.0
36	22915.5
37	25726.0
38	30371.5
39	35017.0
40	39666.5
41	44316.0
42	48451.0
43	52586.0
44	59060.5
45	65535.0
46	71792.0
47	78049.0
48	76806.5
49	75564.0
50	74282.5
51	73001.0
52	70181.0
53	67361.0
54	66691.0
55	66021.0
56	65678.0
57	65335.0
58	62913.5
59	60492.0
60	56273.5
61	52055.0
62	47449.5
63	42844.0
64	36821.5
65	30799.0
66	26510.5
67	22222.0
68	20184.0
69	18146.0
70	16308.5
71	14471.0
72	12054.0
73	9637.0
74	7539.5
75	4093.0
76	2744.0
77	2095.0
78	1446.0
79	1095.5
80	745.0
81	509.0
82	273.0
83	197.0
84	121.0
85	80.5
86	40.0
87	30.0
88	20.0
89	12.0
90	4.0
91	4.5
92	5.0
93	4.0
94	3.0
95	2.5
96	2.0
97	2.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1212239.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.56125875126314
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.01524773506185	29.37375816634495
2	9.643829028161429	8.209070052410421
3	5.65136621474041	7.215877792911394
4	4.0146508177263724	6.834743689968892
5	2.9097989951332335	6.192235397301552
6	2.1111737895556177	5.391252835569683
7	1.5849778167219422	4.72210556807603
8	1.1991294206114829	4.082916603751806
9	0.8937904287653727	3.4236761137276908
>10	2.9035656268375134	17.378476756880268
>50	0.04361384438734639	1.2701964481102423
>100	0.026108066767717594	2.1476915023477137
>500	0.0017667099833830408	0.45667458716825415
>1k	5.889033277943469E-4	0.30361424254837827
>5k	0.0	0.0
>10k+	3.9260221852956465E-4	2.997710242882722
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22937	1.892118633371802	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12943	1.0676937468601488	No Hit
GAATCTGACTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTC	1560	0.12868749479269354	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.003712139272866159	0.0
2	0.0	0.0	0.0	0.026892386732319286	0.0
3	0.0	0.0	0.0	0.05048509411097977	0.0
4	0.0	0.0	0.0	0.10212507599574011	0.0
5	0.0	0.0	0.0	0.24648604771831298	0.0
6	0.0	0.0	0.0	0.398601265921984	0.0
7	0.0	0.0	0.0	0.5206894020073599	0.0
8	0.0	0.0	0.0	0.7464699617814639	0.0
9	0.0	0.0	0.0	1.1219734722278363	0.0
10	0.0	0.0	0.0	1.7232575424483125	0.0
11	0.0	0.0	0.0	1.9655364989907105	0.0
12	0.0	0.0	0.0	2.200638652938901	0.0
13	0.0	0.0	0.0	2.308290691852019	0.0
14	0.0	0.0	0.0	2.3638077969773286	0.0
15	0.0	0.0	0.0	2.409755831977028	0.0
16	0.0	0.0	0.0	2.464200541312398	0.0
17	0.0	0.0	0.0	2.519140202550817	0.0
18	0.0	0.0	0.0	2.6204403587081426	0.0
19	0.0	0.0	0.0	2.6735651963020493	0.0
20	0.0	0.0	0.0	2.7337843445063226	0.0
21	0.0	0.0	0.0	2.7897139095508394	0.0
22	0.0	0.0	0.0	2.8504280096581613	0.0
23	0.0	0.0	0.0	2.9180714364081672	0.0
24	0.0	0.0	0.0	2.976475760967928	0.0
25	0.0	0.0	0.0	3.0276207909496393	0.0
26	0.0	0.0	0.0	3.081735532349644	0.0
27	0.0	0.0	0.0	3.1487190232289177	0.0
28	0.0	0.0	0.0	3.213475230544472	0.0
29	0.0	0.0	0.0	3.283675908793563	0.0
30	0.0	0.0	0.0	3.36823019223107	0.0
31	0.0	8.24919838414702E-5	0.0	3.4385133624640027	0.0
32	0.0	8.24919838414702E-5	0.0	3.5143234956143137	0.0
33	0.0	8.24919838414702E-5	0.0	3.5907935646353564	0.0
34	0.0	8.24919838414702E-5	0.0	3.6678410775432897	0.0
35	0.0	8.24919838414702E-5	0.0	3.748600729724089	0.0
36	8.24919838414702E-5	8.24919838414702E-5	0.0	3.8355472806929987	0.0
37	8.24919838414702E-5	8.24919838414702E-5	0.0	3.9316504418683116	0.0
38	8.24919838414702E-5	8.24919838414702E-5	0.0	4.057450717226554	0.0
39	8.24919838414702E-5	8.24919838414702E-5	0.0	4.2363758301787025	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGA	40	6.8157533E-9	45.000004	2
TTGTCGA	20	7.033439E-4	45.000004	2
GATTACG	55	1.8189894E-12	45.0	1
GTTAACG	25	3.8909337E-5	44.999996	1
CGTTTTT	19740	0.0	43.780396	1
GTTTTTA	13140	0.0	43.33904	2
TAACGCC	175	0.0	42.428574	12
AGCGATA	215	0.0	41.860462	8
TATACGA	60	3.6379788E-12	41.249996	2
TAGCCGT	160	0.0	40.781254	44
TATTTTT	14395	0.0	40.748524	7
TTATTTT	14525	0.0	40.35284	6
GAGCGAT	1040	0.0	40.024036	7
TTTTATT	14710	0.0	39.92182	4
CAACCCG	210	0.0	39.642853	23
TTTATTT	14795	0.0	39.631634	5
GTAGCAT	1055	0.0	39.454975	29
CGCATCG	155	0.0	39.19355	21
ATCGCCA	155	0.0	39.19355	24
ATAACGC	190	0.0	39.07895	11
>>END_MODULE