FastQCFastQC Report
Thu 26 May 2016
SRR1546866_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546866_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1235774
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86200.6975385466921945No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47970.38817777360585354No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC20250.16386491381110138No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACCG207.033493E-445.01
TACGATC207.033493E-445.011
TTAACGA351.212029E-745.02
TATCGCG253.8909773E-545.01
ACATACG1250.043.217
CGTTTTT69950.042.8448871
GTAACGA650.041.5384642
AACACGT2800.040.9821441
ACGAGAT1650.040.909095
CACGACG2550.040.58823426
TCACGAC2500.040.50000425
GTTTTTA53550.040.4621852
AGACTTA5750.040.3043488
GAGGGGA96400.040.145237
AGGGGAA98600.040.1394548
TATGCGA451.9284926E-840.02
AGCGATA2250.040.08
CGGTCTA2600.039.80769331
GCCAGTA8700.039.05172325
GAGCGAT9450.039.0476237
Produced by FastQC (version 0.11.5)