##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546866_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1235774
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.7071406260368	31.0	31.0	34.0	30.0	34.0
2	32.06268864695325	33.0	31.0	34.0	30.0	34.0
3	32.048464363224994	33.0	31.0	34.0	30.0	34.0
4	35.81976235136845	37.0	35.0	37.0	35.0	37.0
5	35.819972745825694	37.0	35.0	37.0	35.0	37.0
6	35.8619820452607	37.0	35.0	37.0	35.0	37.0
7	36.08613872763143	37.0	35.0	37.0	35.0	37.0
8	13.173666867890084	0.0	0.0	35.0	0.0	37.0
9	24.865182468639087	18.0	17.0	38.0	17.0	39.0
10	32.612658139756945	32.0	32.0	37.0	27.0	39.0
11	35.535597932955376	37.0	35.0	37.0	32.0	39.0
12	36.62602870751448	37.0	35.0	39.0	33.0	39.0
13	36.853028142686284	39.0	35.0	39.0	33.0	39.0
14	38.04465784196787	40.0	37.0	41.0	33.0	41.0
15	38.34218635446287	40.0	37.0	41.0	34.0	41.0
16	38.42199301814086	40.0	37.0	41.0	34.0	41.0
17	38.38536172471665	40.0	37.0	41.0	34.0	41.0
18	38.3422567556851	40.0	37.0	41.0	34.0	41.0
19	38.30023369968943	40.0	37.0	41.0	34.0	41.0
20	38.24938783305038	40.0	37.0	41.0	34.0	41.0
21	38.17596502273069	40.0	37.0	41.0	34.0	41.0
22	38.115084958900255	40.0	37.0	41.0	33.0	41.0
23	37.98834900232567	40.0	36.0	41.0	33.0	41.0
24	37.85684194682847	40.0	35.0	41.0	33.0	41.0
25	37.73776273007848	40.0	35.0	41.0	33.0	41.0
26	37.67702427790195	40.0	35.0	41.0	33.0	41.0
27	37.60247019276988	40.0	35.0	41.0	33.0	41.0
28	37.50613057080016	40.0	35.0	41.0	33.0	41.0
29	37.47257346408	40.0	35.0	41.0	33.0	41.0
30	37.373968055647715	40.0	35.0	41.0	33.0	41.0
31	37.28121484996448	40.0	35.0	41.0	33.0	41.0
32	37.085970412065635	40.0	35.0	41.0	32.0	41.0
33	36.890804467483534	40.0	35.0	41.0	31.0	41.0
34	36.634743084091426	40.0	35.0	41.0	31.0	41.0
35	36.44229122800771	40.0	35.0	41.0	30.0	41.0
36	36.300491028294815	39.0	35.0	41.0	30.0	41.0
37	36.227261619033904	39.0	35.0	41.0	30.0	41.0
38	36.13450679493176	39.0	35.0	41.0	29.0	41.0
39	36.068955164941165	39.0	35.0	41.0	29.0	41.0
40	35.95742991841551	39.0	35.0	41.0	29.0	41.0
41	35.890198369604796	39.0	35.0	41.0	28.0	41.0
42	35.81421441137295	39.0	35.0	41.0	28.0	41.0
43	35.678217052632604	39.0	35.0	40.0	27.0	41.0
44	35.48757863492839	38.0	35.0	40.0	27.0	41.0
45	35.383678569058745	38.0	35.0	40.0	26.0	41.0
46	35.357037775515586	38.0	35.0	40.0	26.0	41.0
47	35.27956972715076	38.0	35.0	40.0	26.0	41.0
48	35.20678700150675	38.0	34.0	40.0	26.0	41.0
49	35.10339430996282	38.0	34.0	40.0	26.0	41.0
50	34.995392361386465	38.0	34.0	40.0	26.0	41.0
51	34.614849478950035	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	4.0
12	3.0
13	6.0
14	13.0
15	25.0
16	58.0
17	156.0
18	340.0
19	815.0
20	1521.0
21	2619.0
22	3989.0
23	6144.0
24	9510.0
25	14438.0
26	17972.0
27	19858.0
28	20223.0
29	21037.0
30	25126.0
31	31632.0
32	41441.0
33	56794.0
34	87078.0
35	131982.0
36	134103.0
37	223124.0
38	274691.0
39	111047.0
40	23.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.973990389828565	22.57589170835444	27.461008242607466	16.989109659209532
2	35.270445890591645	23.653839617923666	28.3849635936668	12.690750897817885
3	29.561068609632507	23.710403358542905	33.31450572677529	13.414022305049306
4	26.271389428811414	26.566427194616494	32.07269290339496	15.089490473177133
5	23.158927117741595	30.88048461935597	31.2248841616671	14.735704101235338
6	20.945334664752615	39.28776620967911	29.02059761736369	10.746301508204574
7	83.58526720905279	4.688802321460073	9.569630045623228	2.1563004238639105
8	31.357189906892362	63.900519026941815	3.5507301496875643	1.191560916478256
9	79.38886883847694	5.550286702908461	11.33750993304601	3.7233345255685912
10	44.4071488799732	25.355283409425994	17.24732839499779	12.990239315603013
11	37.94868641029832	22.843497273773362	24.11565545156315	15.092160864365168
12	33.499895611980826	21.32711968369621	28.467098352935082	16.705886351387875
13	23.06376408631352	30.979046330477907	29.169815840113163	16.787373743095422
14	18.23399747850335	33.86484907434531	31.231924283890095	16.669229163261242
15	17.28697965809282	23.182798796543704	42.53107768896254	16.999143856400927
16	19.906228808827503	19.98973922416235	41.08518224206044	19.018849724949707
17	19.67252911940209	19.823284840108304	31.540233084690243	28.96395295579936
18	23.14767910637382	22.386698538729576	33.382883925377946	21.082738429518667
19	27.499041086800663	24.231696086824936	27.42831618079034	20.84094664558406
20	28.277500578584757	24.223846755151023	26.735713811748752	20.76293885451547
21	23.892961010670234	26.125893569536178	29.940102316442974	20.04104310335061
22	23.286782210986797	22.689909319988928	29.019950249802957	25.003358219221315
23	21.111546285971382	27.35095575728248	28.363843226997815	23.173654729748318
24	21.789744726786616	24.0802120776129	34.71597557482193	19.414067620778557
25	20.752904657324073	24.145110675576603	33.45878777187414	21.643196895225177
26	19.114498282048338	29.152660599753677	29.124014585191144	22.608826533006845
27	20.592681186042107	27.37806427388827	30.494168027487227	21.535086512582396
28	19.06109045828768	26.27567823890129	35.22885252481441	19.434378777996624
29	19.87620713819841	24.519127283791374	34.77270115733136	20.83196442067886
30	21.63138243724176	25.833849878699507	32.65799409924469	19.876773584814053
31	28.087336357618785	24.362221571258175	26.585686379548363	20.964755691574673
32	28.479802941314514	24.536606207931225	27.91003856692243	19.073552283831834
33	26.628655401392166	24.566223273834858	27.148896157388002	21.656225167384974
34	21.50830167975698	26.845118929513	30.240966390294666	21.405613000435356
35	21.852377538287744	25.89243664294604	30.619190887654213	21.635994931112
36	26.84293406399552	25.971981931971378	27.459147060870354	19.725936943162747
37	21.93766821441461	29.376973459548427	28.565093617441377	20.120264708595585
38	22.413968897225544	29.84914717415968	25.554187092461888	22.18269683615289
39	21.92747217533303	28.011918036793137	27.203355953434848	22.857253834438986
40	23.34723015697045	25.01978517107497	27.792217670868624	23.840767001085958
41	20.164042939890304	24.248770406239327	28.529245638765666	27.057941015104703
42	23.415041908957463	25.825353179464855	25.728490808189846	25.031114103387836
43	23.047175292569676	25.917441214979437	26.327467643760105	24.70791584869078
44	21.992694457077103	28.004230547009406	28.312782110644825	21.690292885268665
45	20.373790029568514	30.665639510136966	26.615869892067646	22.344700568226877
46	23.588374573344318	28.093081744720312	27.590562675699605	20.72798100623577
47	22.228740853910182	26.73733223065059	28.2550854767943	22.778841438644932
48	23.523395054435518	25.314256490264402	29.113575783274285	22.04877267202579
49	22.457666207575173	24.556593681368923	30.085031729102568	22.900708381953336
50	21.294508542824172	28.646742851039104	28.227896039243422	21.8308525668933
51	20.317145368004184	29.900936579018495	26.98511216452199	22.79680588845533
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1318.0
1	1981.0
2	2644.0
3	12404.5
4	22165.0
5	15047.0
6	7929.0
7	7878.0
8	7827.0
9	8008.0
10	8189.0
11	8170.5
12	8152.0
13	7681.0
14	7210.0
15	7069.0
16	6928.0
17	6371.5
18	5815.0
19	5888.0
20	5961.0
21	6337.5
22	6714.0
23	6670.5
24	6627.0
25	7711.0
26	10232.0
27	11669.0
28	13090.0
29	14511.0
30	16451.5
31	18392.0
32	21109.5
33	23827.0
34	26209.0
35	28591.0
36	32935.5
37	37280.0
38	46579.5
39	55879.0
40	67996.5
41	80114.0
42	88200.0
43	96286.0
44	97462.0
45	98638.0
46	99655.0
47	100672.0
48	96311.5
49	91951.0
50	86143.0
51	80335.0
52	75542.0
53	70749.0
54	67364.0
55	63979.0
56	60708.5
57	57438.0
58	54031.5
59	50625.0
60	46148.0
61	41671.0
62	36503.5
63	31336.0
64	26964.5
65	22593.0
66	19664.0
67	16735.0
68	14682.0
69	12629.0
70	11008.0
71	9387.0
72	7871.0
73	6355.0
74	5049.0
75	2832.0
76	1921.0
77	1494.5
78	1068.0
79	880.5
80	693.0
81	473.5
82	254.0
83	182.0
84	110.0
85	74.5
86	39.0
87	28.0
88	17.0
89	10.5
90	4.0
91	4.0
92	4.0
93	3.5
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1235774.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.88652130080813
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.17283503532424	20.40535822853089
2	6.1454223249319675	3.4274890113335426
3	2.9075721344374648	2.432462167818796
4	1.9862464409182303	2.2155801473328225
5	1.5510051041188724	2.1626068436836534
6	1.2919316755098422	2.1616488112976375
7	1.1063344466952794	2.159627337950998
8	0.9764338110535511	2.178347381661929
9	0.848076989903801	2.128492533930983
>10	9.753431550655767	54.19146833611751
>50	0.2357159649766101	4.058885651091924
>100	0.02354135162502124	1.1092188777262073
>500	5.812679397823269E-4	0.11727602757380794
>1k	5.812679397823269E-4	0.5529074361496322
>5k	2.9063396989116347E-4	0.6986312077997405
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8620	0.6975385466921945	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4797	0.38817777360585354	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2025	0.16386491381110138	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.092094509190192E-5	0.0	0.0	0.00882038301501731	0.0
2	8.092094509190192E-5	0.0	0.0	0.030183512519279414	0.0
3	8.092094509190192E-5	0.0	0.0	0.05203216769409293	0.0
4	8.092094509190192E-5	0.0	0.0	0.08213475926828044	0.0
5	8.092094509190192E-5	0.0	0.0	0.14687151534180198	0.0
6	8.092094509190192E-5	0.0	0.0	0.26849569581493055	0.0
7	8.092094509190192E-5	0.0	0.0	0.34876927334609725	0.0
8	1.6184189018380384E-4	0.0	0.0	0.5657183271374863	0.0
9	1.6184189018380384E-4	0.0	0.0	0.9066382688096691	0.0
10	1.6184189018380384E-4	0.0	0.0	1.3975047217371461	0.0
11	1.6184189018380384E-4	0.0	0.0	1.673040539775072	0.0
12	1.6184189018380384E-4	0.0	0.0	1.9342533505317316	0.0
13	1.6184189018380384E-4	0.0	0.0	2.0472999108251186	0.0
14	1.6184189018380384E-4	0.0	0.0	2.0966616873311787	0.0
15	1.6184189018380384E-4	0.0	0.0	2.147480040848893	0.0
16	1.6184189018380384E-4	0.0	0.0	2.2471746452021164	0.0
17	1.6184189018380384E-4	0.0	0.0	2.357874498087838	0.0
18	1.6184189018380384E-4	0.0	0.0	2.533634790827449	0.0
19	1.6184189018380384E-4	0.0	0.0	2.608810348817826	0.0
20	1.6184189018380384E-4	0.0	0.0	2.6974187836934584	0.0
21	1.6184189018380384E-4	0.0	0.0	2.7923390522862594	0.0
22	1.6184189018380384E-4	0.0	0.0	2.897536280905732	0.0
23	1.6184189018380384E-4	0.0	0.0	3.007103240560167	0.0
24	1.6184189018380384E-4	0.0	0.0	3.1011333787569573	0.0
25	1.6184189018380384E-4	0.0	0.0	3.1773609090335286	0.0
26	1.6184189018380384E-4	0.0	0.0	3.2552877791570305	0.0
27	1.6184189018380384E-4	0.0	0.0	3.3349949100725538	0.0
28	1.6184189018380384E-4	0.0	0.0	3.4224704517168996	0.0
29	1.6184189018380384E-4	0.0	0.0	3.5209512418937443	0.0
30	2.4276283527570574E-4	0.0	0.0	3.6470260743469276	0.0
31	2.4276283527570574E-4	0.0	0.0	3.756431192111179	0.0
32	2.4276283527570574E-4	0.0	0.0	3.8713389341416797	0.0
33	2.4276283527570574E-4	0.0	0.0	3.9824433917528608	0.0
34	2.4276283527570574E-4	0.0	0.0	4.0912820629014695	0.0
35	2.4276283527570574E-4	0.0	0.0	4.216547685903733	0.0
36	2.4276283527570574E-4	0.0	0.0	4.319802811841	0.0
37	2.4276283527570574E-4	0.0	0.0	4.439161205851556	0.0
38	2.4276283527570574E-4	0.0	0.0	4.564588670744003	0.0
39	2.4276283527570574E-4	0.0	0.0	4.72610687714744	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAACCG	20	7.033493E-4	45.0	1
TACGATC	20	7.033493E-4	45.0	11
TTAACGA	35	1.212029E-7	45.0	2
TATCGCG	25	3.8909773E-5	45.0	1
ACATACG	125	0.0	43.2	17
CGTTTTT	6995	0.0	42.844887	1
GTAACGA	65	0.0	41.538464	2
AACACGT	280	0.0	40.98214	41
ACGAGAT	165	0.0	40.90909	5
CACGACG	255	0.0	40.588234	26
TCACGAC	250	0.0	40.500004	25
GTTTTTA	5355	0.0	40.462185	2
AGACTTA	575	0.0	40.304348	8
GAGGGGA	9640	0.0	40.14523	7
AGGGGAA	9860	0.0	40.139454	8
TATGCGA	45	1.9284926E-8	40.0	2
AGCGATA	225	0.0	40.0	8
CGGTCTA	260	0.0	39.807693	31
GCCAGTA	870	0.0	39.051723	25
GAGCGAT	945	0.0	39.047623	7
>>END_MODULE