FastQCFastQC Report
Thu 26 May 2016
SRR1546865_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546865_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1143893
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175701.5359828235682884No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97390.8513908206449379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGCG406.8157533E-945.0000041
CGTTTTT159900.044.0994381
GTTTTTA109650.042.7222982
CACGATA750.042.023
ATAACGC1200.041.25000411
AGCGATA1650.040.909098
CGGTCTA1650.040.9090931
AGTACCT5050.040.5445568
TATTTTT120600.040.4850777
CATATGC7850.040.41401333
TTATTTT121450.040.146156
ACGGGTA451.9283107E-840.05
CGATAGT451.9283107E-840.010
AACACGT1750.039.85714341
TAACGCC1250.039.60000212
TTTATTT124050.039.413545
TCACGAC1600.039.37500425
TACGCGA403.458208E-739.3750042
CACGACC1950.039.2307727
AGACTTA7350.039.1836748
Produced by FastQC (version 0.11.5)