##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546865_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1143893
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.700689662407235	31.0	31.0	34.0	30.0	34.0
2	32.05309762364137	33.0	31.0	34.0	30.0	34.0
3	31.940129015563517	31.0	31.0	34.0	30.0	34.0
4	35.79293605258534	37.0	35.0	37.0	35.0	37.0
5	35.83366276391236	37.0	35.0	37.0	35.0	37.0
6	35.8868556761865	37.0	35.0	37.0	35.0	37.0
7	36.0716386934792	37.0	35.0	37.0	35.0	37.0
8	13.34620283540506	0.0	0.0	35.0	0.0	37.0
9	24.93839895864386	18.0	17.0	39.0	17.0	39.0
10	32.70941075782438	32.0	32.0	38.0	27.0	39.0
11	35.63522462328207	37.0	35.0	37.0	32.0	39.0
12	36.76905881931265	38.0	35.0	39.0	33.0	39.0
13	37.06084572595514	39.0	37.0	39.0	33.0	39.0
14	38.29050968928038	40.0	38.0	41.0	34.0	41.0
15	38.50353048755434	40.0	38.0	41.0	34.0	41.0
16	38.53826013447062	40.0	38.0	41.0	34.0	41.0
17	38.4649228555468	40.0	38.0	41.0	34.0	41.0
18	38.365113695074626	40.0	38.0	41.0	34.0	41.0
19	38.273579784123164	40.0	37.0	41.0	34.0	41.0
20	38.18386509927065	40.0	36.0	41.0	34.0	41.0
21	38.13166703529089	40.0	36.0	41.0	33.0	41.0
22	38.08695481133288	40.0	36.0	41.0	33.0	41.0
23	37.86584584397317	39.0	36.0	41.0	33.0	41.0
24	37.520087980256896	39.0	35.0	41.0	33.0	41.0
25	37.38777228289709	39.0	35.0	41.0	33.0	41.0
26	37.32084032335192	39.0	35.0	41.0	33.0	41.0
27	37.275368412954705	39.0	35.0	41.0	33.0	41.0
28	37.10842709938779	39.0	35.0	41.0	33.0	41.0
29	36.97622155219063	39.0	35.0	41.0	33.0	41.0
30	36.78607964206442	39.0	35.0	41.0	32.0	41.0
31	36.57478540387956	39.0	35.0	41.0	31.0	41.0
32	36.193028543753655	39.0	35.0	41.0	30.0	41.0
33	35.7422424999541	39.0	35.0	41.0	26.0	41.0
34	35.25487261483373	39.0	35.0	41.0	23.0	41.0
35	34.903046001680224	39.0	35.0	41.0	21.0	41.0
36	34.70176318938922	39.0	34.0	41.0	18.0	41.0
37	34.628477488716165	39.0	34.0	41.0	18.0	41.0
38	34.49199619195152	38.0	34.0	40.0	18.0	41.0
39	34.37279535760775	38.0	34.0	40.0	18.0	41.0
40	34.24373433529185	38.0	34.0	40.0	18.0	41.0
41	34.128010224732556	38.0	34.0	40.0	17.0	41.0
42	34.034938582542246	38.0	34.0	40.0	15.0	41.0
43	33.89951420281442	38.0	33.0	40.0	15.0	41.0
44	33.6452072003238	38.0	33.0	40.0	15.0	41.0
45	33.57967222458744	37.0	33.0	40.0	15.0	41.0
46	33.558665889204676	37.0	33.0	40.0	15.0	41.0
47	33.52571612904354	37.0	33.0	40.0	15.0	41.0
48	33.43956209190895	37.0	33.0	40.0	15.0	41.0
49	33.32460990669582	37.0	33.0	40.0	15.0	41.0
50	33.218204849579465	36.0	33.0	40.0	15.0	41.0
51	32.880429375824484	36.0	32.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	6.0
14	13.0
15	29.0
16	54.0
17	159.0
18	471.0
19	989.0
20	1868.0
21	3385.0
22	5656.0
23	9096.0
24	14723.0
25	23435.0
26	30476.0
27	31900.0
28	29440.0
29	26706.0
30	27367.0
31	31524.0
32	38835.0
33	52047.0
34	76727.0
35	111490.0
36	118629.0
37	189448.0
38	223398.0
39	95988.0
40	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.235794781504914	20.09479907648705	23.970423807121822	23.698982334886217
2	42.086279048827116	20.956592968048586	24.77417031138402	12.182957671740276
3	27.539901022210994	22.01499615785742	37.800213831188756	12.644888988742828
4	24.48052396509114	23.86796667170793	37.110988527773145	14.54052083542779
5	21.242371445581014	27.938015181489877	36.41686766157324	14.40274571135587
6	19.413266800303873	35.595287321454016	35.05782446435112	9.933621413890984
7	75.62333190254684	4.227580726519001	17.9001882169049	2.248899154029267
8	28.564647217877898	63.49745998970183	6.701850610153222	1.2360421822670478
9	70.76929398116782	5.524467760533546	19.66302792306623	4.043210335232404
10	38.182155149126714	22.451050928714487	25.692350595728797	13.674443326430008
11	32.437824167120525	21.387664755357363	31.07187472954201	15.1026363479801
12	29.321273930341384	19.194190365707282	35.29796930307293	16.186566400878405
13	23.69067736230574	22.233722909397994	35.760949669243544	18.314650059052724
14	19.05116999579506	25.570486050705792	38.02951849517394	17.34882545832521
15	19.467729936279003	20.3079308991313	42.6913181565059	17.5330210080838
16	22.858606530505913	18.677708491965596	40.430529778571945	18.033155198956546
17	21.30688796941672	18.824925058550058	37.478680261178276	22.38950671085495
18	23.65990525337597	20.750891910344762	37.19482504045396	18.39437779582531
19	26.05164993578945	23.46749215180091	32.654889924145	17.825967988264637
20	26.854609653175604	23.147969259362544	32.205459776395166	17.791961311066682
21	24.312676098201493	24.214415159459847	34.442557127283756	17.030351615054904
22	22.147963139909066	20.998817197063012	35.58820623956961	21.265013423458313
23	22.22043495326923	23.14858120471058	34.37323246142777	20.257751380592413
24	23.049183796036868	21.933257743512723	36.40899979281279	18.60855866763762
25	23.28513243808643	21.136417479606923	34.468433673429246	21.110016408877406
26	19.220853698728817	23.4603236491525	35.024691994793216	22.294130657325468
27	20.04121014815197	21.695648106947065	36.1012787035151	22.161863041385864
28	19.32077563198656	24.08852925929261	37.0406148127491	19.55008029597174
29	21.717940401768345	23.71908911060737	35.992265010800836	18.57070547682344
30	22.65273063127408	21.805710848829392	36.64888236924258	18.89267615065395
31	25.635789361417544	21.87302483711326	33.13544186388063	19.355743937588567
32	26.629413765098658	22.69679069633261	32.367712714388496	18.306082824180233
33	25.699081994557183	22.901093021812354	31.596311892808153	19.803513090822307
34	21.491870306051354	25.058025532108335	34.648520447279594	18.801583714560717
35	21.08475180807995	25.823132058680315	33.188331426103666	19.90378470713607
36	23.37578777035964	28.454497055231563	29.075971266543288	19.09374390786551
37	23.115448735152675	28.913718328549958	28.362530411498277	19.608302524799086
38	23.416438425621976	29.14800597608343	26.774269971054988	20.66128562723961
39	23.48908508051015	26.231998971931812	27.24878987807426	23.03012606948377
40	23.3316402845371	24.21799941078405	29.583885905412483	22.866474399266366
41	20.84285855407805	25.363386260777887	29.256844827269685	24.53691035787438
42	21.9519657870098	27.118270677414756	27.273092850467656	23.656670685107787
43	21.40934510483061	25.42991346218571	28.60494819008421	24.55579324289947
44	22.08265982919731	25.330690895040007	29.93697837122878	22.649670904533902
45	22.039124288722807	26.557204214030506	29.491045054039144	21.91262644320754
46	22.646698598557734	26.87279317208865	29.627421445886988	20.853086783466633
47	21.278301379587077	26.34346044603822	30.444193643985933	21.934044530388768
48	21.819698171070197	25.155674525501947	31.495253489618346	21.529373813809507
49	23.159771062503225	24.343797890187282	31.162530061815225	21.33390098549427
50	20.99409647580674	24.680105569314613	31.184997198164517	23.140800756714132
51	20.675098107952405	26.233222862627887	31.198285154293277	21.89339387512643
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	747.0
1	1968.0
2	3189.0
3	25914.0
4	48639.0
5	33135.5
6	17632.0
7	17083.5
8	16535.0
9	16215.5
10	15896.0
11	15458.5
12	15021.0
13	14133.0
14	13245.0
15	12421.0
16	11597.0
17	10809.5
18	10022.0
19	9284.0
20	8546.0
21	8352.0
22	8158.0
23	7998.5
24	7839.0
25	8452.5
26	9749.5
27	10433.0
28	11399.5
29	12366.0
30	13654.5
31	14943.0
32	16894.5
33	18846.0
34	20615.0
35	22384.0
36	24640.0
37	26896.0
38	31913.5
39	36931.0
40	42887.5
41	48844.0
42	54158.0
43	59472.0
44	62738.0
45	66004.0
46	68943.5
47	71883.0
48	71082.0
49	70281.0
50	68357.0
51	66433.0
52	64684.0
53	62935.0
54	62750.5
55	62566.0
56	62857.0
57	63148.0
58	62744.5
59	62341.0
60	57397.5
61	52454.0
62	46132.0
63	39810.0
64	33893.0
65	27976.0
66	23459.0
67	18942.0
68	16628.5
69	14315.0
70	12916.0
71	11517.0
72	9571.0
73	7625.0
74	5947.5
75	3192.0
76	2114.0
77	1592.5
78	1071.0
79	800.5
80	530.0
81	388.5
82	247.0
83	182.0
84	117.0
85	79.5
86	42.0
87	27.5
88	13.0
89	9.5
90	6.0
91	4.0
92	2.0
93	2.5
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1143893.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.43920153618898
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.25961742384732	28.286240432099657
2	9.493776530652418	7.8682903798649315
3	5.689968473137232	7.073632508786858
4	3.9291272647348774	6.512795863387341
5	2.9419321833384635	6.095566032558152
6	2.23930552361769	5.567701973657678
7	1.6874369544365382	4.8948228024156055
8	1.2786271612303917	4.238823089909712
9	0.9841526953495976	3.6704251696478014
>10	3.431556889842132	20.19014738853074
>50	0.038622472572236624	1.0965835034930056
>100	0.02460381651187137	1.8948038973692263
>500	8.484071528526883E-4	0.20988917050108685
>1k	0.0	0.0
>5k	2.1210178821317208E-4	0.855992726763017
>10k+	2.1210178821317208E-4	1.5442850610151155
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17570	1.5359828235682884	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9739	0.8513908206449379	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.004633300492266322	0.0
2	0.0	0.0	0.0	0.01870804349707534	0.0
3	0.0	0.0	0.0	0.0403009722063165	0.0
4	0.0	0.0	0.0	0.06110711403951244	0.0
5	0.0	0.0	0.0	0.11382183473454248	0.0
6	0.0	0.0	0.0	0.23105307926528093	0.0
7	0.0	0.0	0.0	0.318823526326326	0.0
8	0.0	0.0	0.0	0.5144711961695718	0.0
9	0.0	0.0	0.0	0.8520901867569781	0.0
10	0.0	0.0	0.0	1.3330792303126253	0.0
11	0.0	0.0	0.0	1.5747976427865193	0.0
12	8.742076400502495E-5	0.0	0.0	1.7723685694378757	0.0
13	8.742076400502495E-5	0.0	0.0	1.8790219015240062	0.0
14	8.742076400502495E-5	0.0	0.0	1.9417900100796142	0.0
15	8.742076400502495E-5	0.0	0.0	1.9887349603503124	0.0
16	8.742076400502495E-5	0.0	0.0	2.0421490471573827	0.0
17	8.742076400502495E-5	0.0	0.0	2.10211969126483	0.0
18	8.742076400502495E-5	0.0	0.0	2.2134063238432264	0.0
19	8.742076400502495E-5	0.0	0.0	2.25956448723788	0.0
20	0.005944611952341697	0.0	0.0	2.3188357652332865	0.0
21	0.005944611952341697	0.0	0.0	2.3815164530248896	0.0
22	0.005944611952341697	0.0	0.0	2.45250211339697	0.0
23	0.005944611952341697	0.0	0.0	2.533716003157638	0.0
24	0.005944611952341697	0.0	0.0	2.5939489095571	0.0
25	0.005944611952341697	0.0	0.0	2.6440410073319796	0.0
26	0.005944611952341697	0.0	0.0	2.7040990722034315	0.0
27	0.007867868760452245	0.0	0.0	2.7710633774312807	0.0
28	0.012238906960703493	0.0	0.0	2.8368037919630593	0.0
29	0.012238906960703493	0.0	0.0	2.905691353999019	0.0
30	0.012238906960703493	0.0	0.0	3.0049139211447224	0.0
31	0.052015354582989844	0.0	0.0	3.081057406593099	0.0
32	0.052015354582989844	0.0	0.0	3.182290651310918	0.0
33	0.052015354582989844	0.0	0.0	3.27731702178438	0.0
34	0.052015354582989844	0.0	0.0	3.3729553376058776	0.0
35	0.052015354582989844	0.0	0.0	3.4700798064154603	0.0
36	0.052015354582989844	0.0	0.0	3.5797928652417665	0.0
37	0.052015354582989844	0.0	0.0	3.6951882737283994	0.0
38	0.052015354582989844	0.0	0.0	3.8881258998874895	0.0
39	0.052015354582989844	0.0	0.0	4.112884684144409	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	40	6.8157533E-9	45.000004	1
CGTTTTT	15990	0.0	44.099438	1
GTTTTTA	10965	0.0	42.722298	2
CACGATA	75	0.0	42.0	23
ATAACGC	120	0.0	41.250004	11
AGCGATA	165	0.0	40.90909	8
CGGTCTA	165	0.0	40.90909	31
AGTACCT	505	0.0	40.544556	8
TATTTTT	12060	0.0	40.485077	7
CATATGC	785	0.0	40.414013	33
TTATTTT	12145	0.0	40.14615	6
ACGGGTA	45	1.9283107E-8	40.0	5
CGATAGT	45	1.9283107E-8	40.0	10
AACACGT	175	0.0	39.857143	41
TAACGCC	125	0.0	39.600002	12
TTTATTT	12405	0.0	39.41354	5
TCACGAC	160	0.0	39.375004	25
TACGCGA	40	3.458208E-7	39.375004	2
CACGACC	195	0.0	39.23077	27
AGACTTA	735	0.0	39.183674	8
>>END_MODULE