FastQCFastQC Report
Thu 26 May 2016
SRR1546864_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546864_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2958960
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT534091.804992294589991No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT331400.044.097011
GTTTTTT386950.038.8189662
TACGGGT4550.038.5714264
ACGGGTA3950.037.5949365
TAGCCGT2550.037.05882344
ACGGGAT6500.036.6923075
GGCGATT11450.036.353718
GGGCGAT34900.036.2965627
GTTAGCG1500.036.0000041
CGACGGT2750.036.028
CAATCGT250.002107643836.028
TAGGGTC13650.035.9340675
TGGGCGA10550.035.8293846
GCGCGAC10050.035.8208969
TAGGGTA19200.035.742195
TACGGGA7450.035.335574
CGTTAGG3250.035.307692
GCGGGAT9500.035.2894745
ACCCGCT9750.035.07692334
CGGTCTA2700.035.00000431
Produced by FastQC (version 0.11.5)