##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546864_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2958960
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01190587233352	33.0	31.0	34.0	30.0	34.0
2	32.41183963284397	34.0	31.0	34.0	31.0	34.0
3	32.39700300105442	34.0	31.0	34.0	31.0	34.0
4	36.05161036310055	37.0	35.0	37.0	35.0	37.0
5	36.0327385297537	37.0	35.0	37.0	35.0	37.0
6	36.08417214156325	37.0	35.0	37.0	35.0	37.0
7	36.296445034741936	37.0	36.0	37.0	35.0	37.0
8	36.1704511044421	37.0	37.0	37.0	35.0	37.0
9	38.00027070322005	39.0	39.0	39.0	35.0	39.0
10	37.69880937897099	39.0	38.0	39.0	35.0	39.0
11	37.56265241841728	39.0	37.0	39.0	35.0	39.0
12	37.25132850731338	39.0	37.0	39.0	35.0	39.0
13	37.181294103333606	39.0	37.0	39.0	34.0	39.0
14	38.450025008786874	40.0	38.0	41.0	34.0	41.0
15	38.52452550896261	40.0	38.0	41.0	34.0	41.0
16	38.56265309433044	40.0	38.0	41.0	34.0	41.0
17	38.49189411144456	40.0	37.0	41.0	34.0	41.0
18	38.26705937221186	40.0	37.0	41.0	34.0	41.0
19	38.08675277800308	40.0	37.0	41.0	34.0	41.0
20	37.85684193094871	40.0	35.0	41.0	34.0	41.0
21	37.7328007137643	40.0	35.0	41.0	34.0	41.0
22	37.70580575607646	40.0	35.0	41.0	34.0	41.0
23	37.628844594046555	39.0	35.0	41.0	34.0	41.0
24	37.59541933652364	39.0	35.0	41.0	34.0	41.0
25	37.50006725335929	39.0	35.0	41.0	33.0	41.0
26	37.46882553329548	39.0	35.0	41.0	33.0	41.0
27	37.40368643036743	39.0	35.0	41.0	33.0	41.0
28	37.31464196879985	39.0	35.0	41.0	33.0	41.0
29	37.183308662503045	39.0	35.0	41.0	33.0	41.0
30	37.045588314813315	39.0	35.0	41.0	33.0	41.0
31	36.776707356638816	39.0	35.0	41.0	32.0	41.0
32	36.41189134020061	39.0	35.0	41.0	31.0	41.0
33	36.041622394354775	39.0	35.0	41.0	29.0	41.0
34	35.65418829588774	39.0	35.0	41.0	25.0	41.0
35	35.407043015113416	39.0	35.0	41.0	23.0	41.0
36	35.25677771919864	39.0	35.0	41.0	23.0	41.0
37	35.00176041582178	39.0	35.0	41.0	21.0	41.0
38	35.064344229053454	39.0	35.0	41.0	22.0	41.0
39	34.95431029821289	39.0	35.0	41.0	21.0	41.0
40	34.850091586233	38.0	35.0	41.0	21.0	41.0
41	34.75323154081163	38.0	35.0	41.0	20.0	41.0
42	34.73107307973071	38.0	35.0	41.0	20.0	41.0
43	34.62284383702382	38.0	35.0	41.0	20.0	41.0
44	34.47779422499797	38.0	34.0	40.0	18.0	41.0
45	34.43431475923973	38.0	34.0	40.0	20.0	41.0
46	34.27056127828696	38.0	34.0	40.0	19.0	41.0
47	34.269696447400435	38.0	34.0	40.0	20.0	41.0
48	34.12477492091816	37.0	34.0	40.0	19.0	41.0
49	34.07702706356287	37.0	34.0	40.0	18.0	41.0
50	33.97079852380566	37.0	34.0	40.0	18.0	41.0
51	33.455231905804744	36.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	6.0
11	4.0
12	9.0
13	15.0
14	35.0
15	69.0
16	158.0
17	379.0
18	888.0
19	1908.0
20	3323.0
21	5634.0
22	9185.0
23	14453.0
24	23538.0
25	41354.0
26	63000.0
27	73514.0
28	68424.0
29	59151.0
30	56760.0
31	60719.0
32	71393.0
33	92277.0
34	164358.0
35	290591.0
36	187496.0
37	246004.0
38	417067.0
39	1006883.0
40	364.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.41847135480034	21.31830102468435	25.76986508773353	21.493362532781788
2	38.88940708897721	22.336800767837346	26.392685267796796	12.381106875388651
3	27.31855111255306	23.310926812123178	36.69789385459757	12.672628220726201
4	24.49130775677941	25.12561846054019	36.25996971909049	14.123104063589912
5	21.88265471652202	29.042028280206562	35.08705761483765	13.988259388433773
6	19.967792737989022	36.83091356422527	32.95958715224268	10.24170654554303
7	78.7774420742423	3.9989726119988105	15.02761105253197	2.1959742612269175
8	79.00414334766269	3.582238354016276	14.868974234190391	2.5446440641306403
9	73.90671046583935	5.14816016438208	17.212365155324843	3.732764214453727
10	35.947157109254604	23.274799253791873	28.08581393462568	12.692229702327845
11	30.553910833536108	23.319713683185984	30.167930624273392	15.958444859004514
12	27.928157190364182	21.91786979208911	32.859619596074296	17.294353421472408
13	22.65664287452348	24.968434855489765	34.62986995430827	17.745052315678482
14	18.475139914023845	28.0663814313137	35.87845053667505	17.5800281179874
15	17.7208208289399	23.707755427582665	42.06589477383946	16.505528969637982
16	20.888927190634547	21.601339659880498	40.18814042771785	17.321592721767107
17	20.688789304350177	21.947339605807446	35.1620501797929	22.201820910049477
18	22.148795522751236	23.532693919485226	35.69024251764134	18.628268040122204
19	24.465318895828265	25.079386000486657	32.484183632086946	17.971111471598128
20	25.774630275502204	24.603509341119853	31.84591883634791	17.775941547030037
21	23.468752534674344	24.976376835104226	34.05811501338308	17.49675561683835
22	21.47879660421229	22.490165463541246	34.636122151026036	21.394915781220426
23	20.43092843431476	25.323458242085056	33.83742936707492	20.408183956525267
24	20.32034904155514	24.268324005731742	36.332900748911776	19.078426203801335
25	20.714609187011654	24.79377889528753	34.91682888582475	19.574783031876063
26	19.348487306350883	27.509091031984212	33.48243977613756	19.659981885527348
27	19.7074309892665	25.794231757103848	35.020243599102386	19.478093654527267
28	18.86233676697218	25.925696866466595	37.1188187741639	18.09314759239733
29	19.89979587422608	24.71337902506286	36.37947116554465	19.00735393516641
30	20.434814934977155	24.728181523237893	35.48182469516317	19.355178846621786
31	23.32998756319788	25.187768675480577	31.869643388217483	19.612600373104065
32	23.9624395057723	26.009848054721928	32.05856111606781	17.969151323437966
33	23.338233703733742	26.203057831129854	31.27696893503123	19.181739530105173
34	20.45103685078541	27.481682753399845	32.50263606131884	19.564644334495902
35	20.916538243166517	27.245079352204826	31.40941411847406	20.428968286154596
36	23.113526374131453	27.651235569254062	29.896010760537482	19.339227296077
37	22.110876794549437	28.129579311650037	30.10990347960094	19.649640414199585
38	21.5635223186525	28.48311568929624	28.754697596452804	21.198664395598456
39	21.237191445643063	27.164510503690487	29.40411495931003	22.19418309135642
40	22.41280720253062	25.948475139914024	30.495005001757374	21.143712655797984
41	20.02132506015627	26.263754832779085	30.655703355232923	23.059216751831723
42	22.220509908886907	27.484724362613893	28.82218752534674	21.47257820315246
43	21.813948143942465	27.117433152188607	29.700401492416255	21.368217211452674
44	21.872820180063265	28.20112471949604	29.759205937221182	20.16684916321951
45	20.965575742828563	28.623739421959066	29.344904966609892	21.065779868602483
46	21.6708911239084	27.980067320950603	29.6046583934896	20.744383161651392
47	21.750006759131587	27.451097682969696	30.176447130072724	20.622448427825994
48	22.020811366155677	26.457741909319495	30.503149755319438	21.018296969205398
49	21.45040825154784	25.891732230243058	30.849724227431256	21.80813529077784
50	20.0084827101414	27.3610998458918	31.10008246140536	21.530334982561442
51	19.85376618812015	28.28960175196691	30.05599940519642	21.800632654716523
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2643.0
1	4006.5
2	5370.0
3	48077.5
4	90785.0
5	62419.0
6	34053.0
7	34051.0
8	34049.0
9	34261.5
10	34474.0
11	33816.5
12	33159.0
13	31882.0
14	30605.0
15	28538.5
16	26472.0
17	24879.5
18	23287.0
19	21940.5
20	20594.0
21	20351.5
22	20109.0
23	20587.5
24	21066.0
25	22689.0
26	26638.0
27	28964.0
28	33708.0
29	38452.0
30	43812.0
31	49172.0
32	56194.0
33	63216.0
34	71779.0
35	80342.0
36	85262.5
37	90183.0
38	101865.5
39	113548.0
40	132555.5
41	151563.0
42	170321.5
43	189080.0
44	202399.5
45	215719.0
46	220101.0
47	224483.0
48	216950.5
49	209418.0
50	197323.0
51	185228.0
52	175625.5
53	166023.0
54	154863.0
55	143703.0
56	138634.0
57	133565.0
58	126105.5
59	118646.0
60	109981.5
61	101317.0
62	89952.0
63	78587.0
64	68027.5
65	57468.0
66	48333.5
67	39199.0
68	33086.5
69	26974.0
70	23750.5
71	20527.0
72	17262.5
73	13998.0
74	11532.5
75	6793.5
76	4520.0
77	3353.5
78	2187.0
79	1858.0
80	1529.0
81	1092.0
82	655.0
83	468.5
84	282.0
85	239.0
86	196.0
87	143.5
88	91.0
89	65.5
90	40.0
91	32.5
92	25.0
93	17.5
94	10.0
95	6.0
96	2.0
97	2.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2958960.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.378832252974128
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.7663602357517	19.202318833647226
2	6.045792644924672	2.947787294537667
3	2.376109535144512	1.7378032731594117
4	1.3945514724152726	1.3599014565660004
5	0.9660317156580047	1.1775362573539647
6	0.6954268518464537	1.0172216739227153
7	0.6311257522134058	1.0770276190619912
8	0.509573542184603	0.9938246324377817
9	0.42914464321101453	0.9415840742193381
>10	6.660213253328466	39.89645098985285
>50	1.3991076923081531	22.557807272066903
>100	0.12117365029403336	4.046332963104423
>500	0.003970850003977552	0.692360944576312
>1k	0.0012763446441356415	0.5055279003093395
>5k	0.0	0.0
>10k+	1.4181607157062682E-4	1.846514815184073
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53409	1.804992294589991	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0138697380160597E-4	0.0	0.0	0.03041609214048179	0.0
2	1.0138697380160597E-4	0.0	0.0	0.09635142076945954	0.0
3	1.0138697380160597E-4	0.0	0.0	0.17029632032876416	0.0
4	1.0138697380160597E-4	0.0	0.0	0.26056452267012736	0.0
5	1.0138697380160597E-4	0.0	0.0	0.4842241868764701	0.0
6	1.0138697380160597E-4	0.0	0.0	0.9565523021602185	0.0
7	1.0138697380160597E-4	0.0	0.0	1.311981236650715	0.0
8	1.0138697380160597E-4	0.0	0.0	1.9942817746775894	0.0
9	1.0138697380160597E-4	0.0	0.0	2.4759712872090196	0.0
10	1.0138697380160597E-4	0.0	0.0	3.0753710763241138	0.0
11	1.0138697380160597E-4	0.0	0.0	3.405689836969746	0.0
12	1.0138697380160597E-4	0.0	0.0	3.6582785843674803	0.0
13	1.0138697380160597E-4	0.0	0.0	3.7985643604509693	0.0
14	1.0138697380160597E-4	0.0	0.0	3.8799781004136586	0.0
15	1.0138697380160597E-4	0.0	0.0	3.94253386324925	0.0
16	1.3518263173547464E-4	0.0	0.0	4.038175575202098	0.0
17	1.3518263173547464E-4	0.0	0.0	4.14328007137643	0.0
18	1.3518263173547464E-4	0.0	0.0	4.30472192932652	0.0
19	1.3518263173547464E-4	0.0	0.0	4.388974504555654	0.0
20	1.3518263173547464E-4	0.0	0.0	4.484751399140238	0.0
21	1.3518263173547464E-4	0.0	0.0	4.589179982155892	0.0
22	1.3518263173547464E-4	0.0	0.0	4.708106902425176	0.0
23	1.3518263173547464E-4	0.0	0.0	4.831292075594128	0.0
24	1.6897828966934327E-4	0.0	0.0	4.9363965717684595	0.0
25	1.6897828966934327E-4	0.0	0.0	5.03328872306486	0.0
26	1.6897828966934327E-4	0.0	0.0	5.1249425473815124	0.0
27	1.6897828966934327E-4	0.0	0.0	5.222747181442128	0.0
28	1.6897828966934327E-4	0.0	0.0	5.320315245897207	0.0
29	1.6897828966934327E-4	0.0	0.0	5.431536756157569	0.0
30	1.6897828966934327E-4	0.0	0.0	5.578514072511964	0.0
31	1.6897828966934327E-4	0.0	0.0	5.697745153702653	0.0
32	1.6897828966934327E-4	0.0	0.0	5.824073323059453	0.0
33	1.6897828966934327E-4	0.0	0.0	5.94756265714981	0.0
34	1.6897828966934327E-4	0.0	0.0	6.069700164922811	0.0
35	1.6897828966934327E-4	0.0	0.0	6.205288344553492	0.0
36	2.0277394760321194E-4	0.0	0.0	6.328574904696245	0.0
37	2.0277394760321194E-4	0.0	0.0	6.4583502311623	0.0
38	2.0277394760321194E-4	0.0	0.0	6.607625652256198	0.0
39	2.0277394760321194E-4	0.0	0.0	6.808236677751642	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	33140	0.0	44.09701	1
GTTTTTT	38695	0.0	38.818966	2
TACGGGT	455	0.0	38.571426	4
ACGGGTA	395	0.0	37.594936	5
TAGCCGT	255	0.0	37.058823	44
ACGGGAT	650	0.0	36.692307	5
GGCGATT	1145	0.0	36.35371	8
GGGCGAT	3490	0.0	36.296562	7
GTTAGCG	150	0.0	36.000004	1
CGACGGT	275	0.0	36.0	28
CAATCGT	25	0.0021076438	36.0	28
TAGGGTC	1365	0.0	35.934067	5
TGGGCGA	1055	0.0	35.829384	6
GCGCGAC	1005	0.0	35.820896	9
TAGGGTA	1920	0.0	35.74219	5
TACGGGA	745	0.0	35.33557	4
CGTTAGG	325	0.0	35.30769	2
GCGGGAT	950	0.0	35.289474	5
ACCCGCT	975	0.0	35.076923	34
CGGTCTA	270	0.0	35.000004	31
>>END_MODULE