FastQCFastQC Report
Thu 26 May 2016
SRR1546863_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546863_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2113138
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93550.4427065340739696No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC47050.22265464915211405No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAGCG551.8189894E-1245.0000041
CGTACCA351.2125747E-745.00000432
CGGTCGA351.2125747E-745.00000444
CTATGCG502.1827873E-1145.01
GGTCGAC253.89192E-545.09
ACACCGT207.0346333E-445.045
CCGTCGA253.89192E-545.045
TACGCTA406.8193913E-945.042
GTTCGAC253.89192E-545.039
CGTCGGT207.0346333E-445.036
CGCACGA253.89192E-545.039
ATCGGAT207.0346333E-445.036
CGACTAT253.89192E-545.042
TATCGGC207.0346333E-445.038
ACGCGCC253.89192E-545.018
ATCTCGC302.166371E-644.99999610
CCGTAGA302.166371E-644.99999624
TCGCTAG302.166371E-644.9999961
TACCGGT600.044.99999640
CGGTCTA2300.044.0217431