##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546862_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1693845
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.941360041798394	33.0	31.0	34.0	30.0	34.0
2	32.32810144966039	33.0	31.0	34.0	31.0	34.0
3	32.39973197075293	34.0	31.0	34.0	30.0	34.0
4	36.00989523834826	37.0	35.0	37.0	35.0	37.0
5	35.96673131248727	37.0	35.0	37.0	35.0	37.0
6	36.02413857230148	37.0	35.0	37.0	35.0	37.0
7	36.231179358205736	37.0	35.0	37.0	35.0	37.0
8	36.0982032004109	37.0	35.0	37.0	35.0	37.0
9	37.91559085984845	39.0	39.0	39.0	35.0	39.0
10	37.651970516782825	39.0	37.0	39.0	35.0	39.0
11	37.406992375335406	39.0	37.0	39.0	35.0	39.0
12	36.717380279777664	39.0	35.0	39.0	33.0	39.0
13	36.52359808601141	39.0	35.0	39.0	33.0	39.0
14	37.6057732555222	40.0	35.0	41.0	33.0	41.0
15	37.764081129028924	40.0	35.0	41.0	33.0	41.0
16	37.8628115323421	40.0	35.0	41.0	34.0	41.0
17	37.8008371486175	40.0	35.0	41.0	34.0	41.0
18	37.718392769114054	39.0	35.0	41.0	33.0	41.0
19	37.67705840853207	39.0	36.0	41.0	34.0	41.0
20	37.54321322198903	39.0	35.0	41.0	34.0	41.0
21	37.420254509710155	39.0	35.0	41.0	33.0	41.0
22	37.37857537141828	39.0	35.0	41.0	33.0	41.0
23	37.30830684035434	39.0	35.0	41.0	33.0	41.0
24	37.287428306604205	39.0	35.0	41.0	33.0	41.0
25	37.16604116669471	39.0	35.0	41.0	33.0	41.0
26	37.12219063727791	39.0	35.0	41.0	33.0	41.0
27	37.04516824148609	39.0	35.0	41.0	33.0	41.0
28	36.99145966720686	39.0	35.0	41.0	33.0	41.0
29	36.906596530379105	38.0	35.0	41.0	32.0	41.0
30	36.85984254757667	38.0	35.0	41.0	32.0	41.0
31	36.692917592813984	38.0	35.0	41.0	32.0	41.0
32	36.54273561040119	38.0	35.0	41.0	31.0	41.0
33	36.4469901319188	38.0	35.0	41.0	31.0	41.0
34	36.29328716618109	38.0	35.0	41.0	31.0	41.0
35	36.17595175473553	38.0	35.0	41.0	31.0	41.0
36	36.07676558362778	38.0	35.0	41.0	30.0	41.0
37	35.796566982220924	38.0	35.0	41.0	30.0	41.0
38	35.889088434892216	38.0	35.0	41.0	30.0	41.0
39	35.78529381377871	38.0	35.0	41.0	30.0	41.0
40	35.64687796108853	38.0	35.0	40.0	29.0	41.0
41	35.57741824074812	37.0	35.0	40.0	29.0	41.0
42	35.578286088750744	37.0	35.0	40.0	29.0	41.0
43	35.453927012211864	37.0	35.0	40.0	29.0	41.0
44	35.30503322322881	37.0	35.0	40.0	29.0	41.0
45	35.24801029610148	36.0	35.0	40.0	29.0	41.0
46	35.09391650357618	36.0	34.0	40.0	28.0	41.0
47	35.07782412204186	36.0	34.0	40.0	28.0	41.0
48	34.94001517258073	36.0	34.0	40.0	28.0	41.0
49	34.88584138454227	36.0	34.0	40.0	28.0	41.0
50	34.765645026552015	35.0	34.0	40.0	27.0	41.0
51	34.22136027794751	35.0	34.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	7.0
13	12.0
14	22.0
15	42.0
16	105.0
17	228.0
18	526.0
19	1141.0
20	1878.0
21	3109.0
22	4691.0
23	6913.0
24	10032.0
25	14467.0
26	19835.0
27	23943.0
28	25248.0
29	27170.0
30	30556.0
31	37161.0
32	46548.0
33	62243.0
34	125607.0
35	262104.0
36	110261.0
37	148180.0
38	234168.0
39	497451.0
40	192.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.598652769291164	23.503685402147187	26.518305984313795	14.37935584424785
2	32.337256360528855	25.28802812535976	28.55981509524189	13.814900418869493
3	29.547567811694698	25.265416847468337	30.67051589726333	14.516499443573643
4	26.488610232931585	26.652320607847823	30.21551558731761	16.643553571902977
5	23.777146078891516	31.407773438537767	29.059447588179555	15.755632894391164
6	21.808725119476694	40.409836791441954	26.773287992702993	11.008150096378358
7	85.49034888080078	4.869630928449769	7.2387379010476165	2.4012822897018324
8	86.10964993845364	4.127709442127231	7.093329082649238	2.6693115367698934
9	81.4434024364685	5.571584176828458	9.064642868739465	3.920370517963568
10	49.770374502979905	23.65582447036181	14.293456603172071	12.28034442348621
11	42.376545669763175	21.460464210125487	20.79121761436259	15.371772505748755
12	37.168572094849296	20.87629033353111	25.868305541534202	16.086832030085397
13	24.485239204295553	32.19916816473762	25.912406389014343	17.403186241952483
14	18.854263524702674	35.31928836463785	28.522975833089802	17.30347227756967
15	17.37898095752563	22.984511569830772	41.94397952587161	17.69252794677199
16	20.537534426113368	19.290726129014164	40.13023623767228	20.041503207200186
17	21.186413160590252	19.461048679188472	28.115028234578727	31.23750992564255
18	24.8793720794996	22.5309871918623	30.950411637428456	21.639229091209643
19	29.304924594635285	24.33640622370996	25.110916288090113	21.24775289356464
20	30.293267683878984	23.455097721456212	24.4207704955294	21.8308640991354
21	26.29797885875036	25.369676682341062	27.33467347956867	20.997670979339905
22	24.560039436902432	22.9434806608633	26.85470040056794	25.641779501666328
23	22.491786438546622	27.103365420094516	25.75908657521792	24.64576156614094
24	23.379293855104805	23.90000265667756	32.631616234071004	20.08908725414663
25	21.639583314884185	24.116020060867434	31.176406341784517	23.067990282463864
26	20.26991843999894	29.0422677399644	26.91916910933409	23.768644710702574
27	21.19786639273369	27.944351460729877	28.67151362727995	22.186268519256487
28	19.215984933686375	26.04258358940753	34.70589103489398	20.03554044201211
29	20.365086533891827	24.537841419964636	33.26685735707813	21.83021468906541
30	22.300564691574497	25.92557170225139	31.14157434712149	20.632289259052627
31	29.552172719463705	23.726787279827846	24.94974451617474	21.77129548453371
32	28.82878893877539	25.572115512340265	25.65500385218246	19.944091696701882
33	26.92271134607948	25.928877789880424	25.00760104968282	22.140809814357276
34	21.96635465464668	26.47148942199552	28.209487881122534	23.35266804223527
35	22.47519696312236	26.20210231750839	28.36009198008082	22.962608739288424
36	28.649728871295782	25.006243192263756	25.572528773293897	20.77149916314657
37	22.835914738361538	29.27239505385676	26.622506781907436	21.269183425874267
38	22.8052153532348	30.77991197541688	22.981795854992633	23.433076816355687
39	22.86029713462566	28.217398876520576	25.346238882542384	23.57606510631138
40	24.254226331216845	25.025489345247053	26.165912465426295	24.554371858109803
41	20.66517302350569	24.37224185211752	26.934932062851086	28.027653061525697
42	23.931056265478837	26.16284252691362	24.140874755364276	25.76522645224327
43	23.683867177929503	25.57996747045922	25.39559404786152	25.340571303749755
44	21.882698830176313	27.931953632121004	27.153488070041824	23.031859467660855
45	21.175727413074984	30.894444296851248	24.37165147932662	23.55817681074715
46	23.792849995129426	28.36564148431527	26.636321505214468	21.205187015340837
47	23.596373930318297	26.178605480430615	27.114700577679773	23.110320011571307
48	24.214494242389357	24.369289988163025	28.413402643098983	23.002813126348634
49	23.131632469322756	23.597259489504648	29.006136925161393	24.264971116011207
50	21.36204906588265	28.403130156537344	27.471994190731735	22.762826586848263
51	21.251413204868214	29.85196402268212	25.337560402516168	23.559062369933496
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1329.0
1	2192.5
2	3056.0
3	9650.0
4	16244.0
5	11189.0
6	6134.0
7	6421.5
8	6709.0
9	7013.0
10	7317.0
11	7267.0
12	7217.0
13	6969.0
14	6721.0
15	6352.5
16	5984.0
17	5671.5
18	5359.0
19	4968.0
20	4577.0
21	4500.5
22	4424.0
23	5474.5
24	6525.0
25	7544.5
26	9730.0
27	10896.0
28	11837.0
29	12778.0
30	15774.0
31	18770.0
32	21760.0
33	24750.0
34	27231.0
35	29712.0
36	34026.5
37	38341.0
38	47468.0
39	56595.0
40	72598.5
41	88602.0
42	108356.5
43	128111.0
44	133134.5
45	138158.0
46	136919.0
47	135680.0
48	132760.5
49	129841.0
50	124406.5
51	118972.0
52	114578.0
53	110184.0
54	105092.5
55	100001.0
56	94834.5
57	89668.0
58	87566.5
59	85465.0
60	80961.0
61	76457.0
62	68404.0
63	60351.0
64	54284.0
65	48217.0
66	40973.0
67	33729.0
68	29084.0
69	24439.0
70	20734.5
71	17030.0
72	14817.0
73	12604.0
74	9795.5
75	5408.0
76	3829.0
77	2896.5
78	1964.0
79	1445.0
80	926.0
81	658.5
82	391.0
83	245.5
84	100.0
85	85.5
86	71.0
87	50.5
88	30.0
89	22.0
90	14.0
91	15.0
92	16.0
93	9.5
94	3.0
95	2.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1693845.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.913075853338224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.94588531434316	16.92827439594475
2	6.021483523690504	2.5185548336113173
3	2.031182497580563	1.2743482093162588
4	1.056828215823723	0.8840611456587849
5	0.7104314851644781	0.7428653768922231
6	0.5211661107977504	0.6539511844381576
7	0.4098567222341886	0.5999955304758914
8	0.34596986898882576	0.5788235290506242
9	0.31027680618450376	0.5839958144941246
>10	4.84079067714183	26.387448438194376
>50	2.3027447086786426	33.950880281823935
>100	0.49765728815075394	13.308243472080322
>500	0.00400874685481982	0.5692411916808932
>1k	0.0014316953052927928	0.4585786919726783
>5k	2.863390610585586E-4	0.5607379043656515
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9364	0.5528250813976485	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2700	0.15940065354267952	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.90372790898813E-5	0.0	0.0	0.018242519238773323	0.0
2	5.90372790898813E-5	0.0	0.0	0.07060858579149804	0.0
3	5.90372790898813E-5	0.0	0.0	0.10886474264174113	0.0
4	5.90372790898813E-5	0.0	0.0	0.16908276731342006	0.0
5	5.90372790898813E-5	0.0	0.0	0.31348795196726975	0.0
6	1.180745581797626E-4	0.0	0.0	0.511853209709271	0.0
7	1.180745581797626E-4	0.0	0.0	0.6630476814584569	0.0
8	1.180745581797626E-4	0.0	0.0	1.1050007527253083	0.0
9	1.180745581797626E-4	0.0	0.0	1.3373714832230812	0.0
10	1.180745581797626E-4	0.0	0.0	1.7032255017430757	0.0
11	1.180745581797626E-4	5.90372790898813E-5	0.0	1.9538387514796218	0.0
12	1.180745581797626E-4	5.90372790898813E-5	0.0	2.175582771741216	0.0
13	1.180745581797626E-4	5.90372790898813E-5	0.0	2.284978849894766	0.0
14	1.7711183726964392E-4	5.90372790898813E-5	0.0	2.3206373664650544	0.0
15	1.7711183726964392E-4	5.90372790898813E-5	0.0	2.3753649241813743	0.0
16	1.7711183726964392E-4	5.90372790898813E-5	0.0	2.476613857820521	0.0
17	1.7711183726964392E-4	5.90372790898813E-5	0.0	2.5834713329732057	0.0
18	1.7711183726964392E-4	5.90372790898813E-5	0.0	2.7488937889830534	0.0
19	1.7711183726964392E-4	5.90372790898813E-5	0.0	2.82528802812536	0.0
20	2.361491163595252E-4	5.90372790898813E-5	0.0	2.915260841458339	0.0
21	2.361491163595252E-4	5.90372790898813E-5	0.0	3.0194616390519795	0.0
22	2.361491163595252E-4	5.90372790898813E-5	0.0	3.141668806768034	0.0
23	2.361491163595252E-4	5.90372790898813E-5	0.0	3.2720821562775817	0.0
24	2.361491163595252E-4	5.90372790898813E-5	0.0	3.3681358093568186	0.0
25	2.361491163595252E-4	5.90372790898813E-5	0.0	3.448308434360877	0.0
26	2.361491163595252E-4	5.90372790898813E-5	0.0	3.5293666185512844	0.0
27	2.361491163595252E-4	5.90372790898813E-5	0.0	3.6148525986734326	0.0
28	2.361491163595252E-4	5.90372790898813E-5	0.0	3.7073640150072764	0.0
29	2.361491163595252E-4	5.90372790898813E-5	0.0	3.8118009617172763	0.0
30	2.361491163595252E-4	5.90372790898813E-5	0.0	3.9412106774822964	0.0
31	2.361491163595252E-4	5.90372790898813E-5	0.0	4.05834063919662	0.0
32	2.361491163595252E-4	5.90372790898813E-5	0.0	4.171219916816474	0.0
33	2.361491163595252E-4	5.90372790898813E-5	0.0	4.285043790901765	0.0
34	2.951863954494065E-4	5.90372790898813E-5	0.0	4.400343596964303	0.0
35	2.951863954494065E-4	5.90372790898813E-5	0.0	4.542800551408186	0.0
36	2.951863954494065E-4	5.90372790898813E-5	0.0	4.654794269841692	0.0
37	2.951863954494065E-4	5.90372790898813E-5	0.0	4.778772555930442	0.0
38	2.951863954494065E-4	5.90372790898813E-5	0.0	4.924063299770641	0.0
39	2.951863954494065E-4	5.90372790898813E-5	0.0	5.119063432604518	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGATC	75	0.0	45.000004	24
GCCCATA	20	7.034235E-4	45.0	31
CGTTAGT	20	7.034235E-4	45.0	14
CTAACGA	25	3.8915914E-5	45.0	23
CCCGTAC	20	7.034235E-4	45.0	21
GCGATCG	40	6.8175723E-9	45.0	9
TATACGA	20	7.034235E-4	45.0	34
CGATCGT	20	7.034235E-4	45.0	10
TAATGCG	50	2.1827873E-11	45.0	1
CGTTTTT	5720	0.0	42.679195	1
TCCGAAC	60	3.6379788E-12	41.249996	36
GTATCGA	45	1.9292202E-8	40.0	17
GTTACGA	85	0.0	39.705883	22
CAATTCG	40	3.4595178E-7	39.375	35
CACGACG	370	0.0	38.91892	26
CGACGGT	365	0.0	38.835617	28
TACGGGA	435	0.0	38.793102	4
TAGTAGG	795	0.0	38.490566	2
CGGTCTA	380	0.0	38.486843	31
TAGCCGT	100	0.0	38.25	44
>>END_MODULE