FastQCFastQC Report
Thu 26 May 2016
SRR1546861_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546861_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences666269
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153952.3106282897748507No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTT9580.14378576821073769No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCT8120.12187269706379857No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC7530.11301741488798067No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACG207.0311484E-445.00000421
CATACGA207.0311484E-445.00000418
CGGTATG207.0311484E-445.0000042
CTTAACG351.2109012E-745.01
GACGTAG253.889034E-545.01
CTAAGCG253.889034E-545.01
GTCGATG302.1641226E-644.9999961
GCGTAAG302.1641226E-644.9999961
CGTTTTT83950.044.035141
CGACGGT700.041.78571328
CGTAAGG700.041.7857132
AGTACGG650.041.5384642
CGGGTAC451.9261279E-840.06
CGAGGGT900.040.04
GGACCGT451.9261279E-840.08
CTCACGA850.039.70588324
TCACGAC800.039.37500425
TTAGACG403.4552977E-739.3750041
TGCGAAG403.4552977E-739.3750041
GTTTTTT95850.039.131462
Produced by FastQC (version 0.11.5)