##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546861_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	666269
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.959493838074412	33.0	31.0	34.0	30.0	34.0
2	32.34782497759914	34.0	31.0	34.0	31.0	34.0
3	32.294457643984636	34.0	31.0	34.0	30.0	34.0
4	35.96926766816406	37.0	35.0	37.0	35.0	37.0
5	35.945634571021614	37.0	35.0	37.0	35.0	37.0
6	36.00250199243849	37.0	35.0	37.0	35.0	37.0
7	36.2757880075465	37.0	36.0	37.0	35.0	37.0
8	36.165032441851565	37.0	37.0	37.0	35.0	37.0
9	38.0070046782906	39.0	39.0	39.0	35.0	39.0
10	37.68356474637121	39.0	38.0	39.0	35.0	39.0
11	37.477292204800165	39.0	37.0	39.0	35.0	39.0
12	37.081082865929524	39.0	35.0	39.0	34.0	39.0
13	36.97578155369678	39.0	35.0	39.0	33.0	39.0
14	38.19136865140056	40.0	37.0	41.0	33.0	41.0
15	38.30359809626442	40.0	37.0	41.0	33.0	41.0
16	38.373368714438165	40.0	37.0	41.0	34.0	41.0
17	38.308762676936794	40.0	36.0	41.0	34.0	41.0
18	38.087410640447025	39.0	36.0	41.0	34.0	41.0
19	37.89395724549694	39.0	36.0	41.0	34.0	41.0
20	37.62414130028562	39.0	35.0	41.0	34.0	41.0
21	37.48724013874276	39.0	35.0	41.0	33.0	41.0
22	37.473724576710005	39.0	35.0	41.0	33.0	41.0
23	37.3979053505416	39.0	35.0	41.0	33.0	41.0
24	37.352821457999696	39.0	35.0	41.0	33.0	41.0
25	37.265463348887614	39.0	35.0	41.0	33.0	41.0
26	37.22011079608987	39.0	35.0	41.0	33.0	41.0
27	37.14307734563667	39.0	35.0	41.0	33.0	41.0
28	37.083496305546255	39.0	35.0	41.0	33.0	41.0
29	36.95097775823279	39.0	35.0	41.0	33.0	41.0
30	36.83344414943514	39.0	35.0	41.0	33.0	41.0
31	36.543781865883	38.0	35.0	41.0	31.0	41.0
32	36.15727281323309	39.0	35.0	41.0	30.0	41.0
33	35.769321400215226	39.0	35.0	41.0	28.0	41.0
34	35.36852682625186	39.0	35.0	41.0	24.0	41.0
35	35.09479204345392	39.0	35.0	41.0	23.0	41.0
36	34.94068311747958	38.0	35.0	41.0	21.0	41.0
37	34.64970604965862	38.0	35.0	41.0	20.0	41.0
38	34.732304819825025	38.0	35.0	41.0	21.0	41.0
39	34.60774251841223	38.0	35.0	41.0	20.0	41.0
40	34.50339427468485	38.0	34.0	41.0	18.0	41.0
41	34.40776623255772	38.0	34.0	41.0	18.0	41.0
42	34.39264171078048	38.0	34.0	41.0	18.0	41.0
43	34.27959878067267	38.0	34.0	40.0	18.0	41.0
44	34.14842653642898	38.0	34.0	40.0	18.0	41.0
45	34.12417056774366	38.0	34.0	40.0	18.0	41.0
46	33.98064445441706	37.0	34.0	40.0	18.0	41.0
47	33.96469744202417	37.0	34.0	40.0	18.0	41.0
48	33.8095288839793	37.0	34.0	40.0	18.0	41.0
49	33.77879054856222	37.0	34.0	40.0	18.0	41.0
50	33.65920671680657	37.0	33.0	40.0	18.0	41.0
51	33.15325941924358	36.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	2.0
13	3.0
14	5.0
15	14.0
16	31.0
17	105.0
18	259.0
19	522.0
20	916.0
21	1499.0
22	2314.0
23	3689.0
24	5880.0
25	10057.0
26	15350.0
27	17818.0
28	16197.0
29	14218.0
30	13678.0
31	14804.0
32	17447.0
33	21963.0
34	38806.0
35	73124.0
36	41690.0
37	51410.0
38	87254.0
39	217133.0
40	78.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.860311675914687	21.58017257293976	26.23354831156785	22.325967439577706
2	38.77187742488395	22.781789337339724	27.004258039920813	11.442075197855521
3	25.974944054128287	23.08211848367551	38.884894839771924	12.058042622424276
4	23.20669279225058	25.082661807768336	38.150506777292655	13.560138622688434
5	21.258230534513835	28.937261076231973	36.59723024784284	13.207278141411352
6	18.76959606405221	37.46985076598191	33.83648346238532	9.924069707580571
7	79.48891513787974	3.318479473005648	15.562783200178906	1.6298221889357003
8	80.24071358565385	2.580339172316287	15.142082252063355	2.036864989966515
9	75.7195667215494	4.559869962432591	16.959366261975266	2.7611970540427366
10	35.9739084363823	30.00409744412542	23.095926720288652	10.926067399203625
11	30.151335271489444	21.45004495181376	33.80781636246021	14.590803414236591
12	28.06974360205863	20.950396911757863	35.135508330719276	15.844351155464235
13	21.099285723934326	27.573697710684424	35.74712315896432	15.579893406416929
14	16.216573185905393	30.663891010988053	36.885702321434735	16.233833481671816
15	15.291721511881837	24.84597062147571	44.94716098152548	14.915146885116974
16	18.446003040813846	21.843429605759837	43.29647634814167	16.414091005284654
17	18.218767494810656	22.138955887186707	36.39100723581616	23.251269382186475
18	19.97931766298597	24.026181617334743	38.07456147592039	17.919939243758904
19	22.787192560362257	26.21778891108546	33.324077812415105	17.670940716137178
20	23.978753326359172	25.55199176308668	33.30246492032497	17.166789990229173
21	21.411321853485603	25.871232190001336	35.89916385123726	16.8182821052758
22	20.236721204198304	22.911466689880513	36.04400024614683	20.807811859774354
23	17.788610906405673	27.027521916823382	35.11704731872562	20.066819858045324
24	18.52705138615184	25.535782094019083	38.76107097883888	17.1760955409902
25	18.735976009689782	25.942524715993088	36.71535070669654	18.606148567620586
26	17.586890580231106	29.08104684444271	34.151671472033065	19.180391103293115
27	17.85194868739203	26.96343368819501	36.49156721984664	18.693050404566325
28	16.486284068446828	26.406901716874113	39.54393795899254	17.562876255686515
29	17.125365280389754	25.018273400083153	38.82320804359801	19.03315327592909
30	18.872707570065543	25.151552901305628	37.52178174280959	18.45395778581924
31	22.706594483609475	24.54429066938429	33.39266872689559	19.356446120110647
32	22.911166510823705	25.147950752623938	32.93009280035541	19.010789936196943
33	21.476610798341213	25.980197187622416	32.59434252531635	19.948849488720022
34	18.404578330974424	27.310890946449558	33.43289272050779	20.851638002068235
35	19.204405427837706	26.809742011109627	33.05496728798729	20.930885273065382
36	23.217949506880856	26.69687468575005	30.68805542506105	19.397120382308046
37	20.212706879653712	28.86401738637097	31.650729660242334	19.272546073732983
38	20.54470491648268	29.896183073203165	29.09155311143097	20.467558898883183
39	20.222312609471548	28.05608545497389	30.54622082071956	21.175381114834998
40	21.35909069760112	26.901746892021087	30.713720734418082	21.025441675959712
41	18.628962175937946	26.781375090241326	31.714067441228693	22.87559529259203
42	21.223259674395777	28.399640385489946	29.409142553533187	20.967957386581094
43	20.925932318628064	28.367821405468362	29.98488598448975	20.72136029141383
44	20.453000214628027	29.726731995635397	30.067285135583376	19.752982654153204
45	18.87015604808268	30.726178165275584	29.180556201774362	21.223109584867373
46	21.6835842580099	28.820191244077094	30.204617054072752	19.29160744384025
47	20.58117667188478	28.27386536068765	30.549672879872848	20.59528508755473
48	21.286147186796924	26.84891537802299	30.89412834755932	20.970809087620765
49	20.545605453653103	25.613978738317407	31.803520800157294	22.036895007872197
50	19.045310527729793	28.192516836292846	31.977324474048768	20.78484816192859
51	18.725019474116312	29.790670134735368	30.22097681266876	21.263333578479564
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	517.0
1	770.0
2	1023.0
3	12336.5
4	23650.0
5	15704.5
6	7759.0
7	7809.0
8	7859.0
9	8184.0
10	8509.0
11	8430.0
12	8351.0
13	8184.0
14	8017.0
15	7559.0
16	7101.0
17	6666.0
18	6231.0
19	5902.5
20	5574.0
21	5563.0
22	5552.0
23	5608.0
24	5664.0
25	5927.0
26	6805.0
27	7420.0
28	8632.5
29	9845.0
30	10933.5
31	12022.0
32	13774.5
33	15527.0
34	17147.5
35	18768.0
36	21011.5
37	23255.0
38	26167.5
39	29080.0
40	32844.5
41	36609.0
42	41024.5
43	45440.0
44	46139.5
45	46839.0
46	47679.5
47	48520.0
48	49837.5
49	51155.0
50	50145.0
51	49135.0
52	46136.5
53	43138.0
54	37535.0
55	31932.0
56	28531.0
57	25130.0
58	23030.5
59	20931.0
60	18293.5
61	15656.0
62	13515.5
63	11375.0
64	9638.0
65	7901.0
66	6332.5
67	4764.0
68	3914.5
69	3065.0
70	2810.5
71	2556.0
72	2337.5
73	2119.0
74	1630.5
75	801.5
76	461.0
77	342.0
78	223.0
79	169.5
80	116.0
81	94.5
82	73.0
83	65.5
84	58.0
85	34.0
86	10.0
87	7.5
88	5.0
89	3.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	666269.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.97167881612975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.09222688173229	27.393140150432206
2	7.565249109180863	5.593999204572932
3	3.6277078856151714	4.023673523571159
4	2.3662974151293237	3.4994395206239766
5	1.7313301058006318	3.2005090298178454
6	1.3383805062085823	2.9689304525587716
7	1.1667308038895956	3.0195197582403703
8	0.9552820602030266	2.825470520690962
9	0.8322789143528828	2.7693673836202755
>10	6.2671267975840745	39.57243858881878
>50	0.03566348767429925	0.8611423746013763
>100	0.01967640690954895	1.477369911756529
>500	0.0016397005757957457	0.46179176457962084
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.0992514394893643E-4	2.3332078161152814
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15395	2.3106282897748507	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTT	958	0.14378576821073769	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCT	812	0.12187269706379857	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	753	0.11301741488798067	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03016799520914225	0.0
2	0.0	0.0	0.0	0.1188709064957247	0.0
3	0.0	0.0	0.0	0.2041217586290222	0.0
4	0.0	0.0	0.0	0.31803971068742504	0.0
5	0.0	0.0	0.0	0.6347286156192169	0.0
6	0.0	0.0	0.0	1.2694572312384338	0.0
7	0.0	0.0	0.0	1.7676043760102902	0.0
8	0.0	0.0	0.0	2.6444274009446636	0.0
9	0.0	0.0	0.0	3.1763446896073506	0.0
10	0.0	0.0	0.0	3.8277332428793773	0.0
11	0.0	0.0	0.0	4.181794440383689	0.0
12	0.0	0.0	0.0	4.487826988798819	0.0
13	0.0	0.0	0.0	4.6401678601285665	0.0
14	0.0	0.0	0.0	4.727369876131112	0.0
15	0.0	0.0	0.0	4.796861327782022	0.0
16	0.0	0.0	0.0	4.90042310238057	0.0
17	0.0	0.0	0.0	5.024547142370424	0.0
18	0.0	0.0	0.0	5.220564066465647	0.0
19	0.0	0.0	0.0	5.322775035308561	0.0
20	0.0	0.0	0.0	5.433090838685276	0.0
21	0.0	0.0	0.0	5.5566145205615145	0.0
22	0.0	0.0	0.0	5.684790977818269	0.0
23	0.0	0.0	0.0	5.838932923488861	0.0
24	0.0	0.0	0.0	5.964707948291156	0.0
25	0.0	0.0	0.0	6.072922498270218	0.0
26	0.0	0.0	0.0	6.169580154562197	0.0
27	0.0	0.0	0.0	6.28394837520581	0.0
28	0.0	0.0	0.0	6.40627134085482	0.0
29	0.0	0.0	0.0	6.54405352792941	0.0
30	0.0	0.0	0.0	6.730014453621585	0.0
31	0.0	0.0	0.0	6.869147446451809	0.0
32	0.0	0.0	0.0	7.009931424094472	0.0
33	0.0	0.0	0.0	7.139758866163667	0.0
34	0.0	0.0	0.0	7.281293291448349	0.0
35	0.0	0.0	0.0	7.432583536079271	0.0
36	0.0	0.0	0.0	7.5771197519320275	0.0
37	0.0	0.0	0.0	7.72435757929605	0.0
38	0.0	0.0	0.0	7.904164834323674	0.0
39	0.0	0.0	0.0	8.145058527411601	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGACG	20	7.0311484E-4	45.000004	21
CATACGA	20	7.0311484E-4	45.000004	18
CGGTATG	20	7.0311484E-4	45.000004	2
CTTAACG	35	1.2109012E-7	45.0	1
GACGTAG	25	3.889034E-5	45.0	1
CTAAGCG	25	3.889034E-5	45.0	1
GTCGATG	30	2.1641226E-6	44.999996	1
GCGTAAG	30	2.1641226E-6	44.999996	1
CGTTTTT	8395	0.0	44.03514	1
CGACGGT	70	0.0	41.785713	28
CGTAAGG	70	0.0	41.785713	2
AGTACGG	65	0.0	41.538464	2
CGGGTAC	45	1.9261279E-8	40.0	6
CGAGGGT	90	0.0	40.0	4
GGACCGT	45	1.9261279E-8	40.0	8
CTCACGA	85	0.0	39.705883	24
TCACGAC	80	0.0	39.375004	25
TTAGACG	40	3.4552977E-7	39.375004	1
TGCGAAG	40	3.4552977E-7	39.375004	1
GTTTTTT	9585	0.0	39.13146	2
>>END_MODULE