FastQCFastQC Report
Thu 26 May 2016
SRR1546859_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546859_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1785722
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105280.5895654530772427No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC31330.1754472420679143No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT61750.042.1214561
CACGACG2750.040.90909226
CGTAAGG2550.040.5882342
ACACGAT451.929402E-840.012
CGGTCTA2950.038.89830431
CAACGTA356.250235E-638.57142618
ATAGGGC7650.038.529414
TAGGGTA8300.038.2228935
ACGGGTG2450.037.653065
TCACGAC3050.037.6229525
TACGCAA301.1401751E-437.49999623
CCGAACA301.1401751E-437.49999636
GTTGATC6100.037.254116
AGGGTAC6350.037.2047236
TAGGGAC11150.036.9282535
CGACCCG6900.036.84782832
TTAGCCG552.750312E-936.818181
TAGCCGT552.750312E-936.8181844
TATAACG552.750312E-936.818181
TCTCACG3150.036.42857423
Produced by FastQC (version 0.11.5)