FastQCFastQC Report
Thu 26 May 2016
SRR1546858_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546858_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1580258
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT403142.55110241492212No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGG253.891472E-545.02
CGTTTTT212100.043.8967441
CGGTCTA2300.041.08695631
CACGACG2350.040.21276526
GCGAATA451.9290383E-840.026
GTTTTTT246800.038.873582
ATACGAG700.038.571431
ATGGGAC8250.038.4545445
GCGCGAC5850.038.0769239
ACACGAC5700.037.89473326
TACGGGA4100.037.8658524
CGATCGA301.1401154E-437.50000410
GCGAGAC5700.037.49999621
TAGGGAC11300.036.8362855
ACGGGCT2700.036.6666685
AACACGT5100.036.6176541
ACGGGAT4500.036.55
TAGCCGT1300.036.34615344
CGACGGT2550.036.1764728
GACGGTC2550.036.1764729
Produced by FastQC (version 0.11.5)