##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546858_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1580258
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.896704209059532	31.0	31.0	34.0	30.0	34.0
2	32.28299619429232	33.0	31.0	34.0	30.0	34.0
3	32.24111569123523	34.0	31.0	34.0	30.0	34.0
4	35.93442083507883	37.0	35.0	37.0	35.0	37.0
5	35.91661614748984	37.0	35.0	37.0	35.0	37.0
6	35.96592834840893	37.0	35.0	37.0	35.0	37.0
7	36.19632173986779	37.0	35.0	37.0	35.0	37.0
8	36.048734447159895	37.0	35.0	37.0	35.0	37.0
9	37.867965863801984	39.0	38.0	39.0	35.0	39.0
10	37.63450082201767	39.0	37.0	39.0	35.0	39.0
11	37.44355984908793	39.0	37.0	39.0	35.0	39.0
12	37.08253462409303	39.0	37.0	39.0	34.0	39.0
13	37.00265020015719	39.0	37.0	39.0	33.0	39.0
14	38.217091133220016	40.0	37.0	41.0	33.0	41.0
15	38.30336375452616	40.0	37.0	41.0	33.0	41.0
16	38.36042089329717	40.0	37.0	41.0	34.0	41.0
17	38.283454980136156	40.0	37.0	41.0	34.0	41.0
18	38.02871429855125	39.0	36.0	41.0	34.0	41.0
19	37.800571805363425	39.0	36.0	41.0	34.0	41.0
20	37.51339401540761	39.0	35.0	41.0	34.0	41.0
21	37.391655666353216	39.0	35.0	41.0	33.0	41.0
22	37.363979805829175	39.0	35.0	41.0	33.0	41.0
23	37.26922375966456	39.0	35.0	41.0	33.0	41.0
24	37.208043243571616	39.0	35.0	41.0	33.0	41.0
25	37.08872222130817	39.0	35.0	41.0	33.0	41.0
26	37.031389178222796	39.0	35.0	41.0	33.0	41.0
27	36.95822454308094	39.0	35.0	41.0	33.0	41.0
28	36.84616182927092	38.0	35.0	41.0	33.0	41.0
29	36.68861983296399	38.0	35.0	41.0	32.0	41.0
30	36.543896629537706	38.0	35.0	41.0	31.0	41.0
31	36.24307359937428	38.0	35.0	41.0	31.0	41.0
32	35.79684013623092	38.0	35.0	41.0	29.0	41.0
33	35.3841410706353	38.0	35.0	41.0	25.0	41.0
34	34.963979932390785	38.0	35.0	41.0	22.0	41.0
35	34.66950713111403	38.0	35.0	41.0	20.0	41.0
36	34.516981404302335	38.0	34.0	41.0	18.0	41.0
37	34.23420795844729	38.0	34.0	41.0	18.0	41.0
38	34.280496602453525	38.0	34.0	41.0	18.0	41.0
39	34.14528197294366	38.0	34.0	40.0	18.0	41.0
40	34.01212966490282	38.0	34.0	40.0	18.0	41.0
41	33.882078116358215	37.0	34.0	40.0	16.0	41.0
42	33.846003627255804	37.0	34.0	40.0	16.0	41.0
43	33.7186756846034	37.0	33.0	40.0	15.0	41.0
44	33.54850980029843	37.0	33.0	40.0	15.0	41.0
45	33.49873248545491	36.0	33.0	40.0	15.0	41.0
46	33.33239572272376	36.0	33.0	40.0	15.0	41.0
47	33.305533653365465	36.0	33.0	40.0	15.0	41.0
48	33.15528730118753	36.0	33.0	40.0	15.0	41.0
49	33.11430918242464	36.0	33.0	40.0	15.0	41.0
50	32.991107781134474	35.0	33.0	40.0	15.0	41.0
51	32.489441597511295	35.0	32.0	39.0	13.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	6.0
12	4.0
13	5.0
14	17.0
15	51.0
16	110.0
17	305.0
18	720.0
19	1529.0
20	2624.0
21	4291.0
22	6615.0
23	9945.0
24	15691.0
25	26077.0
26	39479.0
27	45947.0
28	42694.0
29	37475.0
30	35524.0
31	37787.0
32	43860.0
33	55299.0
34	94268.0
35	158400.0
36	111855.0
37	139292.0
38	220927.0
39	449277.0
40	184.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.59768847871677	20.732880327136456	24.365325155765703	23.304106038381075
2	40.70284725658722	22.094619992431618	25.319283306903053	11.88324944407812
3	27.22625039708706	22.850572501452294	37.45331458533986	12.469862516120786
4	24.0773342074522	24.534854435161854	37.387755670276626	14.00005568710932
5	21.47364544270619	28.298606936335712	36.446200557124214	13.78154706383388
6	19.301721617609278	36.151248720145695	34.58087223731821	9.966157424926815
7	76.10345905542006	4.179444116087374	17.4532259922114	2.263870836281164
8	76.00391834751035	4.141349070847925	17.21541672309205	2.63931585854968
9	71.46738064290767	5.580417881130803	18.989430839774265	3.9627706361872557
10	37.55279201244354	26.583001003633584	24.069550668308594	11.794656315614285
11	30.793389433877255	22.546887913239484	31.821196285669807	14.838526367213456
12	27.071845230335807	20.74743491252694	35.36947764225842	16.811242214878835
13	22.234090888956107	24.907388540352272	36.10859745687097	16.749923113820657
14	18.076478650954463	27.653712241925057	37.06685870281941	17.20295040430107
15	17.369632047425167	23.03263138044547	42.16887369024552	17.428862881883845
16	20.295736518973484	21.607484347492626	40.57021068711565	17.526568446418242
17	20.57853844119125	21.544709787895393	35.670694279035445	22.20605749187791
18	22.5251193159598	22.56416357328993	36.2023163306245	18.70840078012578
19	23.884770714655453	24.757602872442348	32.98834747237476	18.369278940527433
20	25.42375991768433	23.715367996871397	32.31136940929899	18.549502676145288
21	23.27423749792755	24.549345739746293	34.50328996910631	17.673126793219843
22	22.34926195595909	21.65867851958351	34.9839076910226	21.008151833434795
23	20.93651796099118	25.46381666791119	33.99172793303372	19.60793743806391
24	20.697569637363014	24.294450652994637	36.71767521505982	18.29030449458253
25	21.03884302436691	24.0730311126411	35.34688639450014	19.541239468491852
26	19.52073648733308	26.209201282322255	33.79024184658454	20.47982038376012
27	20.115955748997948	24.48948209722716	35.5987440025616	19.795818151213282
28	19.014869723804594	25.312512260656174	37.16545019863845	18.507167816900786
29	19.60363434325281	24.269454734606626	36.81588702604258	19.31102389609798
30	21.3126590721262	23.798392414403217	35.781435689615236	19.107512823855345
31	24.552319937630436	23.010166694299286	32.358197205772726	20.079316162297552
32	23.865786472841776	24.806202531485365	32.00173642531789	19.32627457035497
33	22.5868813826603	24.942699230125715	31.26748923277085	21.202930154443138
34	20.295483395749304	26.25495330509322	32.814704940585656	20.63485835857183
35	21.16331636985859	26.289821029224342	31.716213428440167	20.8306491724769
36	23.553052729364445	27.19790059597863	29.26977746671746	19.979269207939463
37	22.111389406033698	27.994289540062447	29.053420390847574	20.840900663056285
38	21.561669043915614	29.02671589069633	27.962396013815464	21.44921905157259
39	21.92616648673824	27.661116096232384	27.918036168777505	22.49468124825187
40	22.821526611477367	26.089790401314218	29.493095431252364	21.59558755595605
41	20.609672597765684	26.143958771289245	29.848290595586292	23.398078035358783
42	21.85763337379086	27.621628873259933	28.49933365311234	22.02140409983686
43	22.17270850709188	26.846438999201393	28.761885717395515	22.218966776311213
44	21.695191544671818	27.941196943790192	29.738245273872998	20.625366237664988
45	20.97530909509713	28.012640973815667	28.966535844146968	22.045514086940234
46	22.547204317269713	27.56708081844863	29.372482214929462	20.513232649352194
47	21.66184255988579	26.981416958496652	30.336375452615965	21.020365029001596
48	22.13265175686502	26.221541039501144	30.333464535537868	21.31234266809597
49	21.989320731171745	25.411103756475207	30.75725609362522	21.84231941872783
50	19.942629621239064	27.04064779295533	31.669575474384565	21.347147111421048
51	20.600053915246754	27.513988222176376	30.045600148836453	21.840357713740417
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1439.0
1	2398.5
2	3358.0
3	32851.5
4	62345.0
5	41829.0
6	21313.0
7	21085.5
8	20858.0
9	21240.5
10	21623.0
11	21535.5
12	21448.0
13	20544.5
14	19641.0
15	18401.5
16	17162.0
17	16070.5
18	14979.0
19	13809.0
20	12639.0
21	12062.0
22	11485.0
23	11303.5
24	11122.0
25	11317.5
26	12327.0
27	13141.0
28	15185.0
29	17229.0
30	19044.5
31	20860.0
32	24250.5
33	27641.0
34	31685.5
35	35730.0
36	39266.5
37	42803.0
38	46870.0
39	50937.0
40	59205.0
41	67473.0
42	75033.0
43	82593.0
44	86082.5
45	89572.0
46	93008.5
47	96445.0
48	103100.5
49	109756.0
50	111705.0
51	113654.0
52	108501.0
53	103348.0
54	93836.5
55	84325.0
56	80447.0
57	76569.0
58	73937.5
59	71306.0
60	65434.0
61	59562.0
62	53967.5
63	48373.0
64	42546.0
65	36719.0
66	31567.5
67	26416.0
68	22835.0
69	19254.0
70	16441.0
71	13628.0
72	11824.5
73	10021.0
74	8245.0
75	4644.0
76	2819.0
77	2099.5
78	1380.0
79	1103.0
80	826.0
81	558.5
82	291.0
83	197.5
84	104.0
85	78.0
86	52.0
87	37.0
88	22.0
89	15.0
90	8.0
91	7.0
92	6.0
93	3.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1580258.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.788516985006353
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.75339668201555	23.631232574011662
2	6.3970735464614945	3.939128150791291
3	2.8196534944807907	2.604388485199631
4	1.7732633627679362	2.1838459665388035
5	1.2914217985436731	1.9880480989634668
6	0.9818419037376587	1.8137673677890727
7	0.8156424735890953	1.757869550625332
8	0.731904233918075	1.8027396749907776
9	0.6328203252800474	1.753523940000779
>10	7.55124103991823	48.71593085126401
>50	0.21649254616003324	4.16660335627112
>100	0.030242878710613683	1.9196753237374156
>500	0.003754285812637961	0.7991687595967553
>1k	0.0010428571701772114	0.33527200432741333
>5k	0.0	0.0
>10k+	2.0857143403544225E-4	2.588805895892615
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40314	2.55110241492212	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8984241813678525E-4	0.0	0.0	0.018351433753222575	0.0
2	1.8984241813678525E-4	0.0	0.0	0.06929248261992663	0.0
3	1.8984241813678525E-4	0.0	0.0	0.13073814529019945	0.0
4	1.8984241813678525E-4	0.0	0.0	0.2304054148120117	0.0
5	2.5312322418238035E-4	0.0	0.0	0.4687842111857684	0.0
6	2.5312322418238035E-4	0.0	0.0	0.9168123179885816	0.0
7	2.5312322418238035E-4	0.0	0.0	1.273083256025282	0.0
8	2.5312322418238035E-4	0.0	0.0	1.9918266510911509	0.0
9	2.5312322418238035E-4	0.0	0.0	2.462952252100606	0.0
10	2.5312322418238035E-4	0.0	0.0	2.994321180465468	0.0
11	2.5312322418238035E-4	0.0	0.0	3.2674411393582568	0.0
12	2.5312322418238035E-4	0.0	0.0	3.5080980447496546	0.0
13	2.5312322418238035E-4	0.0	0.0	3.6386463476217177	0.0
14	2.5312322418238035E-4	0.0	0.0	3.7148997189066595	0.0
15	2.5312322418238035E-4	0.0	0.0	3.772168848377923	0.0
16	3.796848362735705E-4	0.0	0.0	3.8499409590079594	0.0
17	3.796848362735705E-4	0.0	0.0	3.9313833563886402	0.0
18	3.796848362735705E-4	0.0	0.0	4.0617418168425665	0.0
19	3.796848362735705E-4	0.0	0.0	4.138627996187965	0.0
20	3.796848362735705E-4	0.0	0.0	4.219247743090053	0.0
21	3.796848362735705E-4	0.0	0.0	4.309233049286888	0.0
22	3.796848362735705E-4	0.0	0.0	4.40402769674319	0.0
23	4.429656423191656E-4	0.0	0.0	4.511858190244884	0.0
24	4.429656423191656E-4	0.0	0.0	4.600198195484535	0.0
25	5.062464483647607E-4	0.0	0.0	4.67999529190803	0.0
26	5.062464483647607E-4	0.0	0.0	4.760868162034301	0.0
27	5.062464483647607E-4	0.0	0.0	4.8468667774502645	0.0
28	5.062464483647607E-4	0.0	0.0	4.932295865611818	0.0
29	5.062464483647607E-4	0.0	0.0	5.032912347224314	0.0
30	5.062464483647607E-4	0.0	0.0	5.173332455839489	0.0
31	5.062464483647607E-4	0.0	0.0	5.275594238409171	0.0
32	5.062464483647607E-4	0.0	0.0	5.394182468938617	0.0
33	5.062464483647607E-4	0.0	0.0	5.511631644959241	0.0
34	5.062464483647607E-4	0.0	0.0	5.625220691811084	0.0
35	5.062464483647607E-4	0.0	0.0	5.740138635589885	0.0
36	5.062464483647607E-4	0.0	0.0	5.854803456144503	0.0
37	5.062464483647607E-4	0.0	0.0	5.971429981686534	0.0
38	5.062464483647607E-4	0.0	0.0	6.1243164090926925	0.0
39	5.062464483647607E-4	0.0	0.0	6.353013242141473	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGG	25	3.891472E-5	45.0	2
CGTTTTT	21210	0.0	43.896744	1
CGGTCTA	230	0.0	41.086956	31
CACGACG	235	0.0	40.212765	26
GCGAATA	45	1.9290383E-8	40.0	26
GTTTTTT	24680	0.0	38.87358	2
ATACGAG	70	0.0	38.57143	1
ATGGGAC	825	0.0	38.454544	5
GCGCGAC	585	0.0	38.076923	9
ACACGAC	570	0.0	37.894733	26
TACGGGA	410	0.0	37.865852	4
CGATCGA	30	1.1401154E-4	37.500004	10
GCGAGAC	570	0.0	37.499996	21
TAGGGAC	1130	0.0	36.836285	5
ACGGGCT	270	0.0	36.666668	5
AACACGT	510	0.0	36.61765	41
ACGGGAT	450	0.0	36.5	5
TAGCCGT	130	0.0	36.346153	44
CGACGGT	255	0.0	36.17647	28
GACGGTC	255	0.0	36.17647	29
>>END_MODULE