FastQCFastQC Report
Thu 26 May 2016
SRR1546857_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546857_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4051093
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT586401.4475105854148498No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT327000.043.582571
TCACGAC6300.039.99999625
CGTAGTC356.2521976E-638.5714269
CGTTAGG5500.038.4545442
ACGGGTA3550.038.028175
CACGACG6700.037.6119426
GTTTTTT395100.037.5284732
CGTATGG3300.037.52
GGCGATA11900.037.24798
ATAGGGC18250.037.232884
CGGTCTA6900.036.84782431
GGGCGAT51700.036.8181847
ATGGGAC23350.036.7130665
TAGGGAC26650.036.4727975
GCGATAT3400.036.397069
TAGGGAT31650.035.7582975
AGGGCGA26650.035.7129486
CGACGGT7000.035.67857428
TATGGGC13750.035.672734
TAACGCC2650.035.66037812
Produced by FastQC (version 0.11.5)