FastQCFastQC Report
Thu 26 May 2016
SRR1546856_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546856_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3038645
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283700.9336398295950992No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC43490.14312300383888213No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34100.11222107222133548No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAA207.0351193E-445.019
CGTTTTT150650.043.1778951
CGGTCTA6250.040.6831
CACGACG6200.040.6451626
TCACGAC6400.039.37525
CATACGA3000.039.00000418
ATAGGGC9250.038.918924
CGACGGT6700.038.61940428
ACATACG3200.037.9687517
TATGGGC10500.037.9285744
ACGGGAC5600.037.767865
GCGCGAC12050.037.1576779
CAATGCG850.037.0588231
ATAACGC2250.037.00000411
GGGCGAT40100.036.976317
ACGGTCT6900.036.84782430
TTAGGGA23450.036.74844
AGGGCGA20150.036.7369736
TAGGGTA15200.036.7105265
TACGGGC4800.036.56254
Produced by FastQC (version 0.11.5)