##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546856_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3038645
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03597590373341	33.0	31.0	34.0	31.0	34.0
2	32.4451721079626	34.0	31.0	34.0	31.0	34.0
3	32.4954757795004	34.0	31.0	34.0	31.0	34.0
4	36.090355076028956	37.0	35.0	37.0	35.0	37.0
5	36.048206026041214	37.0	35.0	37.0	35.0	37.0
6	36.093386690449194	37.0	35.0	37.0	35.0	37.0
7	36.30073338609808	37.0	36.0	37.0	35.0	37.0
8	36.18423310389993	37.0	37.0	37.0	35.0	37.0
9	38.022992814231344	39.0	39.0	39.0	35.0	39.0
10	37.77013438555672	39.0	38.0	39.0	35.0	39.0
11	37.526274704679224	39.0	37.0	39.0	35.0	39.0
12	36.945790969330076	39.0	35.0	39.0	34.0	39.0
13	36.78558304770712	39.0	35.0	39.0	33.0	39.0
14	37.87024479661165	40.0	35.0	41.0	33.0	41.0
15	38.0188064087776	40.0	35.0	41.0	33.0	41.0
16	38.118959931153526	40.0	35.0	41.0	34.0	41.0
17	38.07785016018653	40.0	35.0	41.0	34.0	41.0
18	37.98034814859913	40.0	36.0	41.0	34.0	41.0
19	37.898866435532945	40.0	36.0	41.0	34.0	41.0
20	37.749725946927	40.0	35.0	41.0	34.0	41.0
21	37.61448869479653	40.0	35.0	41.0	33.0	41.0
22	37.60056669996001	39.0	35.0	41.0	33.0	41.0
23	37.53652236440914	39.0	35.0	41.0	33.0	41.0
24	37.50335001291694	39.0	35.0	41.0	33.0	41.0
25	37.38849191004543	39.0	35.0	41.0	33.0	41.0
26	37.36530624669877	39.0	35.0	41.0	33.0	41.0
27	37.29947822137828	39.0	35.0	41.0	33.0	41.0
28	37.23627867026257	39.0	35.0	41.0	33.0	41.0
29	37.13075828206322	39.0	35.0	41.0	33.0	41.0
30	37.06678832176842	39.0	35.0	41.0	33.0	41.0
31	36.85968844665961	39.0	35.0	41.0	32.0	41.0
32	36.70075642268182	39.0	35.0	41.0	32.0	41.0
33	36.563385324708875	39.0	35.0	41.0	31.0	41.0
34	36.361807318722654	39.0	35.0	41.0	31.0	41.0
35	36.226215961390686	39.0	35.0	41.0	31.0	41.0
36	36.12629872854512	39.0	35.0	41.0	30.0	41.0
37	35.872723862116175	38.0	35.0	41.0	30.0	41.0
38	35.9331995017516	38.0	35.0	41.0	30.0	41.0
39	35.845432421358865	38.0	35.0	41.0	30.0	41.0
40	35.70588831535109	38.0	35.0	41.0	29.0	41.0
41	35.62271933707294	38.0	35.0	41.0	29.0	41.0
42	35.61247957560031	38.0	35.0	41.0	29.0	41.0
43	35.504069083423694	38.0	35.0	40.0	29.0	41.0
44	35.36761517057767	37.0	35.0	40.0	29.0	41.0
45	35.31663586894817	37.0	35.0	40.0	28.0	41.0
46	35.15482756294335	37.0	35.0	40.0	28.0	41.0
47	35.13149709821318	37.0	35.0	40.0	28.0	41.0
48	34.98186099396277	36.0	35.0	40.0	27.0	41.0
49	34.94717382254262	36.0	34.0	40.0	27.0	41.0
50	34.823461444163435	36.0	34.0	40.0	27.0	41.0
51	34.307470928654055	35.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	8.0
12	10.0
13	23.0
14	52.0
15	116.0
16	194.0
17	421.0
18	931.0
19	1760.0
20	2980.0
21	4836.0
22	7672.0
23	11268.0
24	17380.0
25	27634.0
26	38936.0
27	45421.0
28	46494.0
29	46976.0
30	51744.0
31	61364.0
32	77034.0
33	105267.0
34	212073.0
35	419713.0
36	195616.0
37	261595.0
38	429545.0
39	971089.0
40	490.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.684352729588355	23.39526334928891	29.10330097790298	15.817082943219757
2	33.10830978939626	25.007988758147135	28.764498649891646	13.119202802564958
3	28.82715815766567	24.511912381999213	32.70852633328342	13.952403127051696
4	26.80856105270606	26.0159709344132	31.580753921567013	15.594714091313728
5	23.30989635182787	30.26213986826365	30.803762861406973	15.624200918501502
6	21.318021684007178	39.08176177210566	28.39107562745895	11.209140916428211
7	84.6810667254648	4.399691309777878	8.602551466196282	2.3166904985610364
8	85.63185235524386	3.620692775891886	8.379820610831473	2.3676342580327745
9	81.172858297037	5.172009234379139	10.12994278699881	3.525189681585049
10	47.119192929743356	24.181172858297035	16.17553218622116	12.524102025738445
11	38.995966952375156	21.54358274823153	23.20119658597829	16.259253713415024
12	34.188988842066124	20.732201359487533	28.425268499610844	16.6535412988355
13	24.963067419853257	28.643227491200847	28.934739003733572	17.458966085212325
14	19.07106621536902	31.263737619893078	31.680304872731103	17.9848912920068
15	18.15506582703804	22.99623680949897	41.45798538493309	17.390711978529904
16	21.957352701615356	19.889588945072557	38.2874274553296	19.865630897982488
17	22.459484408346484	20.328073861869353	29.03442159251903	28.17802013726513
18	24.692782473767092	22.602969415644143	31.917746232284454	20.786501878304307
19	28.246340062758236	24.228134579722212	26.922592142221287	20.602933215298265
20	28.925919283101514	24.117822253010797	26.25334647515587	20.70291198873182
21	25.421923258557676	24.832844902909027	30.034143508043883	19.711088330489414
22	24.539457554271724	22.017116181719153	29.704884907582162	23.73854135642696
23	22.576839347801407	25.639421518472872	28.733728355895472	23.05001077783025
24	22.864763735151687	23.95837618412154	32.72369756914678	20.453162511579997
25	22.50516266296326	24.029230133826097	31.10053987879466	22.365067324415982
26	20.670891137332596	27.697246634601935	28.170944615116277	23.460917612949192
27	20.556892957222708	26.49944300831456	31.35739778750068	21.586266246962047
28	19.263388780196436	25.38091813949968	34.37453206939277	20.981161010911116
29	20.60257121183949	24.85657258416169	33.46748303931522	21.073373164683602
30	22.5290219818373	25.051527901416588	31.88967450952645	20.529775607219666
31	27.927711200222465	23.61039213201937	27.640477910384398	20.821418757373763
32	28.18802459648955	24.808590671170865	27.718868113912613	19.284516618426963
33	26.967085658245697	24.430790697827486	26.941877053752577	21.66024659017424
34	21.728731062694063	25.97907949102314	29.98092899960344	22.31126044667936
35	22.46790921611442	25.922376585616284	29.842577859539364	21.767136338729927
36	27.95940295756826	24.70574877947243	27.31329260245932	20.021555660499992
37	23.51982544851406	27.6077988708783	27.773793911430918	21.09858176917672
38	23.320789364996568	29.09369143154268	25.203042803618057	22.382476399842695
39	23.062549261266124	26.70466606003663	26.27170992333754	23.961074755359707
40	23.924742771860483	24.44342790947939	27.066241696545664	24.565587622114464
41	20.971880558604248	23.45713303133469	28.379754792020783	27.19123161804028
42	22.68744785916091	25.53766563715077	27.037873789139567	24.737012714548754
43	23.03947976812033	24.93591716044487	27.66051973823859	24.36408333319621
44	22.538269524738823	26.588726225011477	28.308209744804014	22.564794505445683
45	21.559807085065877	29.0649615206778	26.197400486071913	23.177830908184404
46	23.820551594542962	26.79082288322591	27.971941441004134	21.416684081226993
47	23.00522107715775	26.02923342476663	28.462127033595568	22.503418464480056
48	23.52436694645146	25.31950918912871	28.892417508461833	22.263706355958
49	23.151141380450827	23.50715532745681	29.971385272053823	23.370318020038535
50	21.392429849488835	26.92443506892052	28.9961150446992	22.687020036891443
51	21.042866145930176	28.617064513952766	27.561166243506563	22.778903096610495
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2491.0
1	4142.5
2	5794.0
3	25847.0
4	45900.0
5	30594.5
6	15289.0
7	15226.0
8	15163.0
9	15770.0
10	16377.0
11	16229.5
12	16082.0
13	15461.5
14	14841.0
15	14074.5
16	13308.0
17	12487.0
18	11666.0
19	11634.0
20	11602.0
21	11482.0
22	11362.0
23	11489.5
24	11617.0
25	14334.5
26	19292.0
27	21532.0
28	23909.0
29	26286.0
30	33408.0
31	40530.0
32	44885.5
33	49241.0
34	54293.0
35	59345.0
36	65788.5
37	72232.0
38	86850.0
39	101468.0
40	126557.5
41	151647.0
42	180498.0
43	209349.0
44	216561.0
45	223773.0
46	225324.5
47	226876.0
48	231957.0
49	237038.0
50	232198.5
51	227359.0
52	212952.0
53	198545.0
54	191010.5
55	183476.0
56	176277.5
57	169079.0
58	159705.5
59	150332.0
60	139903.0
61	129474.0
62	118369.0
63	107264.0
64	95088.0
65	82912.0
66	70275.0
67	57638.0
68	48218.0
69	38798.0
70	32615.0
71	26432.0
72	22428.5
73	18425.0
74	14401.5
75	7924.0
76	5470.0
77	4113.5
78	2757.0
79	2071.0
80	1385.0
81	970.0
82	555.0
83	398.0
84	241.0
85	182.0
86	123.0
87	86.5
88	50.0
89	41.5
90	33.0
91	27.0
92	21.0
93	24.0
94	27.0
95	17.0
96	7.0
97	4.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3038645.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.3806339959224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.74180776301077	15.648274244240376
2	6.627835972573741	2.5690332633892012
3	2.1779857134478897	1.2663223188204442
4	1.1818430871162806	0.9161947324804668
5	0.7601280241897928	0.736588151343301
6	0.5790408116798392	0.6733306823921276
7	0.4242215495009946	0.5755177808843297
8	0.3435767942629512	0.5326988879282071
9	0.27834119433768906	0.4854985932101994
>10	3.866833388257319	18.556533348337503
>50	1.9434354474934072	27.535498294035737
>100	1.0680196233255648	28.04856889602845
>500	0.00433164425249844	0.6204913276464249
>1k	0.002425720781399126	0.8827847446962258
>5k	0.0	0.0
>10k+	1.732657700999376E-4	0.9526647345670025
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28370	0.9336398295950992	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4349	0.14312300383888213	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	3410	0.11222107222133548	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.581881068700029E-5	0.0	0.0	0.014085225487018063	0.0
2	6.581881068700029E-5	0.0	0.0	0.06693773046867929	0.0
3	6.581881068700029E-5	0.0	0.0	0.11607147264652501	0.0
4	6.581881068700029E-5	0.0	0.0	0.18531286148924933	0.0
5	6.581881068700029E-5	0.0	0.0	0.35199899955407754	0.0
6	6.581881068700029E-5	0.0	0.0	0.6330124117822253	0.0
7	6.581881068700029E-5	0.0	0.0	0.8254337048256707	0.0
8	6.581881068700029E-5	0.0	0.0	1.2067878939461503	0.0
9	6.581881068700029E-5	0.0	0.0	1.4538058904544624	0.0
10	6.581881068700029E-5	0.0	0.0	1.810280569135256	0.0
11	6.581881068700029E-5	0.0	0.0	2.1103485270572904	0.0
12	6.581881068700029E-5	0.0	0.0	2.350751733091559	0.0
13	6.581881068700029E-5	0.0	0.0	2.450927962957173	0.0
14	6.581881068700029E-5	0.0	0.0	2.501443900159446	0.0
15	9.872821603050043E-5	0.0	0.0	2.5528483913059934	0.0
16	9.872821603050043E-5	0.0	0.0	2.6522347954433636	0.0
17	1.3163762137400058E-4	0.0	0.0	2.7610991083196623	0.0
18	1.3163762137400058E-4	0.0	0.0	2.925448678605102	0.0
19	1.3163762137400058E-4	0.0	0.0	3.011309317146294	0.0
20	1.3163762137400058E-4	0.0	0.0	3.112308282145496	0.0
21	1.3163762137400058E-4	0.0	0.0	3.2267671939301894	0.0
22	1.3163762137400058E-4	0.0	0.0	3.34468159327595	0.0
23	1.3163762137400058E-4	0.0	0.0	3.4734890057904098	0.0
24	1.3163762137400058E-4	0.0	0.0	3.576363806894191	0.0
25	1.3163762137400058E-4	0.0	0.0	3.664297737972024	0.0
26	1.3163762137400058E-4	0.0	0.0	3.7614133931406926	0.0
27	1.3163762137400058E-4	0.0	0.0	3.8730091866605014	0.0
28	1.6454702671750074E-4	0.0	0.0	3.974699249171917	0.0
29	1.6454702671750074E-4	0.0	0.0	4.088170878796306	0.0
30	1.6454702671750074E-4	0.0	0.0	4.2243499981077095	0.0
31	1.6454702671750074E-4	0.0	0.0	4.3386772722710285	0.0
32	1.6454702671750074E-4	0.0	0.0	4.448660504929006	0.0
33	1.6454702671750074E-4	0.0	0.0	4.566081263194615	0.0
34	1.6454702671750074E-4	0.0	0.0	4.689228257989992	0.0
35	1.6454702671750074E-4	0.0	0.0	4.831034885615134	0.0
36	1.6454702671750074E-4	0.0	0.0	4.953589511114329	0.0
37	1.6454702671750074E-4	0.0	0.0	5.086872602755505	0.0
38	1.6454702671750074E-4	0.0	0.0	5.260798809995903	0.0
39	1.6454702671750074E-4	0.0	0.0	5.564157708452287	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCTAA	20	7.0351193E-4	45.0	19
CGTTTTT	15065	0.0	43.177895	1
CGGTCTA	625	0.0	40.68	31
CACGACG	620	0.0	40.64516	26
TCACGAC	640	0.0	39.375	25
CATACGA	300	0.0	39.000004	18
ATAGGGC	925	0.0	38.91892	4
CGACGGT	670	0.0	38.619404	28
ACATACG	320	0.0	37.96875	17
TATGGGC	1050	0.0	37.928574	4
ACGGGAC	560	0.0	37.76786	5
GCGCGAC	1205	0.0	37.157677	9
CAATGCG	85	0.0	37.058823	1
ATAACGC	225	0.0	37.000004	11
GGGCGAT	4010	0.0	36.97631	7
ACGGTCT	690	0.0	36.847824	30
TTAGGGA	2345	0.0	36.7484	4
AGGGCGA	2015	0.0	36.736973	6
TAGGGTA	1520	0.0	36.710526	5
TACGGGC	480	0.0	36.5625	4
>>END_MODULE