FastQCFastQC Report
Thu 26 May 2016
SRR1546855_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546855_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4467646
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1050772.3519544744592564No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT53530.11981701325485501No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCT45990.10294011656250295No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC44700.10005268994007135No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT502450.043.7864461
GTACGAG4350.039.8275831
GTTTTTT582750.038.7451742
ACGGGAC9700.038.0412375
GTTAGCG2800.037.7678571
CGTAAGG4800.037.5000042
AGGGCGA27800.037.149286
CGACGGT5850.036.92307728
TAGGGAC29750.036.5294155
TAACGCG1050.036.428571
GCGCGAC13800.036.3586969
TCTAGCG1550.036.290321
TGGGCGA16600.036.054226
CTATACG1000.036.01
ATTACGC504.8843503E-836.014
TTACGAG1750.036.01
TATGGGC13900.035.9352534
TCACGAC6100.035.7786925
ATAGGGA40150.035.6973844
TACGCGG3100.035.5645142
Produced by FastQC (version 0.11.5)