##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546855_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4467646
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95766517759017	33.0	31.0	34.0	30.0	34.0
2	32.344307942034796	34.0	31.0	34.0	31.0	34.0
3	32.31018684112394	34.0	31.0	34.0	30.0	34.0
4	35.976030106234916	37.0	35.0	37.0	35.0	37.0
5	35.950049086252584	37.0	35.0	37.0	35.0	37.0
6	36.002507360699575	37.0	35.0	37.0	35.0	37.0
7	36.2515044835692	37.0	36.0	37.0	35.0	37.0
8	36.117321739457424	37.0	36.0	37.0	35.0	37.0
9	37.918213305172344	39.0	39.0	39.0	35.0	39.0
10	37.68609889861462	39.0	38.0	39.0	35.0	39.0
11	37.48706119509021	39.0	37.0	39.0	35.0	39.0
12	37.07777854377898	39.0	35.0	39.0	34.0	39.0
13	36.977476057861345	39.0	35.0	39.0	33.0	39.0
14	38.17982355808853	40.0	37.0	41.0	33.0	41.0
15	38.268882986700376	40.0	37.0	41.0	33.0	41.0
16	38.3318873070964	40.0	37.0	41.0	34.0	41.0
17	38.26771816746448	40.0	36.0	41.0	34.0	41.0
18	38.05272888675602	39.0	36.0	41.0	34.0	41.0
19	37.873764617877065	39.0	36.0	41.0	34.0	41.0
20	37.631173329310336	39.0	35.0	41.0	34.0	41.0
21	37.498003870494664	39.0	35.0	41.0	33.0	41.0
22	37.48040623630431	39.0	35.0	41.0	33.0	41.0
23	37.39617395827691	39.0	35.0	41.0	33.0	41.0
24	37.35739156593875	39.0	35.0	41.0	33.0	41.0
25	37.25471445141356	39.0	35.0	41.0	33.0	41.0
26	37.21188451367902	39.0	35.0	41.0	33.0	41.0
27	37.14509990272282	39.0	35.0	41.0	33.0	41.0
28	37.04428282813813	39.0	35.0	41.0	33.0	41.0
29	36.90451571140596	39.0	35.0	41.0	33.0	41.0
30	36.77999152126198	38.0	35.0	41.0	32.0	41.0
31	36.503363068604806	38.0	35.0	41.0	31.0	41.0
32	36.15499594193452	38.0	35.0	41.0	30.0	41.0
33	35.81141545234336	38.0	35.0	41.0	29.0	41.0
34	35.458207073702795	38.0	35.0	41.0	25.0	41.0
35	35.20591067421188	38.0	35.0	41.0	23.0	41.0
36	35.08281318618351	38.0	35.0	41.0	23.0	41.0
37	34.81024929011833	38.0	35.0	41.0	22.0	41.0
38	34.88616152667423	38.0	35.0	41.0	23.0	41.0
39	34.77396038092544	38.0	35.0	41.0	22.0	41.0
40	34.66972293686653	38.0	35.0	41.0	21.0	41.0
41	34.56551526239993	38.0	34.0	40.0	21.0	41.0
42	34.53495196351725	38.0	34.0	40.0	21.0	41.0
43	34.41441622724808	38.0	34.0	40.0	20.0	41.0
44	34.26793976067039	37.0	34.0	40.0	20.0	41.0
45	34.2240103625041	37.0	34.0	40.0	20.0	41.0
46	34.05782754497559	37.0	34.0	40.0	20.0	41.0
47	34.04283777183779	37.0	34.0	40.0	20.0	41.0
48	33.87913635055239	36.0	34.0	40.0	20.0	41.0
49	33.84994155758984	36.0	34.0	40.0	20.0	41.0
50	33.7345020173935	36.0	34.0	40.0	19.0	41.0
51	33.17720540078601	35.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	4.0
11	9.0
12	20.0
13	32.0
14	55.0
15	122.0
16	276.0
17	710.0
18	1559.0
19	3204.0
20	5837.0
21	9674.0
22	15086.0
23	22880.0
24	37096.0
25	63026.0
26	93698.0
27	107343.0
28	101798.0
29	93074.0
30	92114.0
31	101179.0
32	120068.0
33	153901.0
34	272150.0
35	503202.0
36	310866.0
37	378188.0
38	605519.0
39	1374325.0
40	625.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.07148596822577	21.880225067071116	25.955838936209357	21.092450028493754
2	38.89907571011669	22.48244377464105	26.374650095374612	12.243830419867644
3	26.98297940347109	22.973865879257218	37.275312323312995	12.767842393958698
4	24.547423855873987	25.1318703406671	36.29020741571736	14.030498387741552
5	21.81739555909309	28.82728398803307	34.898646848922226	14.45667360395161
6	19.910462914922086	36.94930171280357	32.80239750418901	10.33783786808534
7	77.81637130605245	4.54431707436086	15.219491427924236	2.419820191662455
8	77.99487694414464	4.041412412711303	15.120378830372863	2.8433318127712
9	72.74206595598667	5.887328584225339	17.21667294141031	4.153932518377687
10	39.16677820937469	26.420356491987057	22.305370658284026	12.107494640354227
11	32.272073481202405	22.3183305033568	30.1110025279532	15.298593487487594
12	29.130709998061622	20.644361706366173	33.777385227030074	16.447543068542135
13	22.712162064765202	25.799873132293833	34.72927353689169	16.75869126604928
14	17.999232705545605	29.36604645936585	35.815572675185095	16.81914815990345
15	16.95387235246481	23.74930780102094	42.86364676162794	16.433173084886313
16	19.859787458540808	21.69610125779885	40.74736449575459	17.696746787905756
17	20.06837605307135	21.51038824472664	34.22802522849841	24.1932104737036
18	22.491553717550588	23.254102943697866	35.824302104508725	18.430041234242818
19	24.764920944945057	25.21770972901613	31.70528730342556	18.312082022613254
20	25.50108043475244	24.623728021423364	31.09022066654341	18.784970877280788
21	23.525968709248673	25.488277271744447	33.215433810109396	17.770320208897484
22	22.085433805632764	22.776692692303733	33.49137330934456	21.64650019271894
23	20.10423386275457	26.376015467653435	32.46465812197295	21.055092547619036
24	20.830432849872167	24.66435344250641	35.66090509409206	18.84430861352936
25	20.865081969341347	24.830682645849738	34.44021751051896	19.86401787428995
26	18.72131319267462	28.307860560124954	32.47443955944584	20.49638668775458
27	19.343050008886113	26.30532947328414	34.33732216026068	20.014298357569064
28	18.49815316611925	25.684980412503588	37.14414705193742	18.672719369439744
29	19.565292326204897	25.11781372114084	35.85577281637802	19.46112113627624
30	20.936506607730333	25.90872240101387	34.22598836165623	18.92878262959957
31	25.33580323955837	24.904099384776682	30.675684689431527	19.08441268623342
32	25.073025033764985	26.425280785451665	30.378548345146417	18.123145835636933
33	23.475047038194162	26.151646750884023	30.370602326146702	20.002703884775112
34	20.668759342168112	27.04475690329986	31.795379490675852	20.491104263856176
35	20.702155005119028	26.753149197586378	32.15890426412478	20.385791533169815
36	24.294382321249266	26.096293215711363	30.17580175331707	19.4335227097223
37	21.315229541463225	28.20579338649481	30.608736681464915	19.870240390577052
38	20.914414436595916	29.370769304461454	28.383560380567307	21.331255878375323
39	20.761291292998596	26.881225593970516	29.668151863419794	22.68933124961109
40	21.92440940933995	25.646100877285267	30.26123824492809	22.168251468446694
41	19.912566931220603	25.34748724496077	30.405340978224327	24.334604845594303
42	21.533151910424415	27.288710878167162	28.327512967679176	22.850624243729246
43	21.5547964185166	26.78126243663889	29.217176114669783	22.446765030174728
44	21.373604802170988	28.079418109671177	29.724915537175505	20.82206155098233
45	20.461759951437514	29.03721557169033	28.650233254828155	21.850791222044002
46	22.45730749481942	27.582959795829844	29.321056323620986	20.638676385729756
47	21.71476880666015	26.936825343816405	29.86004262647488	21.48836322304856
48	21.661855035067685	26.1385973732028	30.33758717678169	21.861960414947827
49	21.423026802034002	24.90893414563285	31.015729536315096	22.652309516018054
50	20.24130828628768	27.257531147275323	30.931434585461787	21.569725980975214
51	19.920960613262555	28.42138790763637	29.546812795821335	22.11083868327974
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4126.0
1	6424.5
2	8723.0
3	81415.5
4	154108.0
5	100634.5
6	47161.0
7	47342.5
8	47524.0
9	49363.5
10	51203.0
11	50886.5
12	50570.0
13	49664.5
14	48759.0
15	46076.5
16	43394.0
17	40452.5
18	37511.0
19	34584.0
20	31657.0
21	30552.0
22	29447.0
23	29635.0
24	29823.0
25	30094.0
26	34622.5
27	38880.0
28	42416.5
29	45953.0
30	51569.0
31	57185.0
32	64991.0
33	72797.0
34	85282.5
35	97768.0
36	105699.5
37	113631.0
38	131758.0
39	149885.0
40	183401.0
41	216917.0
42	256607.0
43	296297.0
44	324034.0
45	351771.0
46	357689.0
47	363607.0
48	357233.0
49	350859.0
50	321871.0
51	292883.0
52	275078.5
53	257274.0
54	236149.5
55	215025.0
56	206494.0
57	197963.0
58	190404.5
59	182846.0
60	169035.5
61	155225.0
62	139795.0
63	124365.0
64	108543.0
65	92721.0
66	77522.0
67	62323.0
68	53155.0
69	43987.0
70	37201.5
71	30416.0
72	24603.5
73	18791.0
74	15322.5
75	8928.0
76	6002.0
77	4502.5
78	3003.0
79	2327.5
80	1652.0
81	1221.5
82	791.0
83	578.5
84	366.0
85	222.5
86	79.0
87	73.5
88	68.0
89	48.5
90	29.0
91	25.0
92	21.0
93	27.5
94	34.0
95	19.0
96	4.0
97	3.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4467646.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.273995542749706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.04423922923945	18.6294926704411
2	7.32091779847413	3.407740164210478
3	2.592459523897885	1.8101067421187518
4	1.3977971732983803	1.3012930072405857
5	0.9075837721818645	1.056155033421634
6	0.6582235463861806	0.9191695130834923
7	0.5261310743699747	0.8571620595852234
8	0.4198249900685118	0.7816803958071586
9	0.3192978398243739	0.6688202850793878
>10	3.730502238963503	20.549699463885016
>50	1.382589011035525	23.156322732051414
>100	0.6927336145810198	21.998371462901726
>500	0.0037513734848066606	0.5858923602611741
>1k	0.0037513734847727034	1.7408355474350066
>5k	9.872035486243957E-5	0.12299144331199059
>10k+	9.872035486243957E-5	2.414267119165708
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	105077	2.3519544744592564	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT	5353	0.11981701325485501	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCT	4599	0.10294011656250295	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4470	0.10005268994007135	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.714945633561836E-5	0.0	0.0	0.05042924170804938	0.0
2	6.714945633561836E-5	0.0	0.0	0.17367087723602093	0.0
3	6.714945633561836E-5	0.0	0.0	0.2985240997160473	0.0
4	6.714945633561836E-5	0.0	0.0	0.44515612920092595	0.0
5	6.714945633561836E-5	0.0	0.0	0.8001081553910045	0.0
6	6.714945633561836E-5	0.0	0.0	1.459291985085658	0.0
7	6.714945633561836E-5	0.0	0.0	1.9400820924486855	0.0
8	6.714945633561836E-5	0.0	0.0	2.782561554787465	0.0
9	6.714945633561836E-5	0.0	0.0	3.348161425502379	0.0
10	6.714945633561836E-5	0.0	0.0	4.043941708899944	0.0
11	6.714945633561836E-5	0.0	0.0	4.508504030981864	0.0
12	6.714945633561836E-5	0.0	0.0	4.899604847832617	0.0
13	8.953260844749114E-5	0.0	0.0	5.091607526648262	0.0
14	8.953260844749114E-5	0.0	0.0	5.196830724726175	0.0
15	8.953260844749114E-5	0.0	0.0	5.295383743474751	0.0
16	8.953260844749114E-5	0.0	0.0	5.447902541965052	0.0
17	8.953260844749114E-5	0.0	0.0	5.615888098564658	0.0
18	8.953260844749114E-5	0.0	0.0	5.885314100535271	0.0
19	8.953260844749114E-5	0.0	0.0	6.012763768660275	0.0
20	1.1191576055936393E-4	0.0	0.0	6.155724961198806	0.0
21	1.1191576055936393E-4	0.0	0.0	6.31545113466913	0.0
22	1.1191576055936393E-4	0.0	0.0	6.485585473871475	0.0
23	1.1191576055936393E-4	0.0	0.0	6.67268624237462	0.0
24	1.1191576055936393E-4	0.0	0.0	6.8305546142196585	0.0
25	1.1191576055936393E-4	0.0	0.0	6.9633090893951755	0.0
26	1.1191576055936393E-4	0.0	0.0	7.098928608041013	0.0
27	1.1191576055936393E-4	0.0	0.0	7.241800267971097	0.0
28	1.3429891267123672E-4	0.0	0.0	7.390133417016478	0.0
29	1.3429891267123672E-4	0.0	0.0	7.5464125850615735	0.0
30	1.3429891267123672E-4	0.0	0.0	7.7648497665213405	0.0
31	1.566820647831095E-4	0.0	0.0	7.930731306822429	0.0
32	1.566820647831095E-4	0.0	0.0	8.10021653461353	0.0
33	1.566820647831095E-4	0.0	0.0	8.26441933850623	0.0
34	1.566820647831095E-4	0.0	0.0	8.435225172271931	0.0
35	1.566820647831095E-4	0.0	0.0	8.623400332076445	0.0
36	1.566820647831095E-4	0.0	0.0	8.79821275007017	0.0
37	1.566820647831095E-4	0.0	0.0	8.9711449832865	0.0
38	1.790652168949823E-4	0.0	0.0	9.18125563216065	0.0
39	1.790652168949823E-4	0.0	0.0	9.454128639556492	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	50245	0.0	43.786446	1
GTACGAG	435	0.0	39.827583	1
GTTTTTT	58275	0.0	38.745174	2
ACGGGAC	970	0.0	38.041237	5
GTTAGCG	280	0.0	37.767857	1
CGTAAGG	480	0.0	37.500004	2
AGGGCGA	2780	0.0	37.14928	6
CGACGGT	585	0.0	36.923077	28
TAGGGAC	2975	0.0	36.529415	5
TAACGCG	105	0.0	36.42857	1
GCGCGAC	1380	0.0	36.358696	9
TCTAGCG	155	0.0	36.29032	1
TGGGCGA	1660	0.0	36.05422	6
CTATACG	100	0.0	36.0	1
ATTACGC	50	4.8843503E-8	36.0	14
TTACGAG	175	0.0	36.0	1
TATGGGC	1390	0.0	35.935253	4
TCACGAC	610	0.0	35.77869	25
ATAGGGA	4015	0.0	35.697384	4
TACGCGG	310	0.0	35.564514	2
>>END_MODULE