FastQCFastQC Report
Thu 26 May 2016
SRR1546854_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546854_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2214326
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135740.6130082020443242No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27780.1254557820302882No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT79250.042.586751
TCGATAG2250.040.01
TCTAACG356.2509134E-638.5714261
TATAGCG1700.038.382351
CACGACG4000.037.687526
ATAGGGC9700.037.5773164
CGGCATA301.1402636E-437.49999644
TCACGAC4150.036.8674725
CGACGGT4150.036.8674728
GTAGGGC10450.036.818184
GGGCGAT28800.036.6406257
GTAGGGA18550.036.630734
TTTCGCG800.036.5625041
TACGGGT4200.036.428574
TTACGGG7850.036.4012763
AGGGCGA16400.036.356716
CTACGGG3900.036.3461533
TAGGGTC9300.036.048395
CGCGAAA250.002107529236.032
ACTACGG1000.036.02
Produced by FastQC (version 0.11.5)