##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546854_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2214326
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.9333268904398	33.0	31.0	34.0	30.0	34.0
2	32.34041058091717	33.0	31.0	34.0	31.0	34.0
3	32.41218185578817	34.0	31.0	34.0	31.0	34.0
4	36.01705801223488	37.0	35.0	37.0	35.0	37.0
5	35.97770427660606	37.0	35.0	37.0	35.0	37.0
6	36.03723796767052	37.0	35.0	37.0	35.0	37.0
7	36.256331723513156	37.0	36.0	37.0	35.0	37.0
8	36.139604105267246	37.0	36.0	37.0	35.0	37.0
9	37.973877378488986	39.0	39.0	39.0	35.0	39.0
10	37.613050652884894	39.0	37.0	39.0	35.0	39.0
11	37.445088482906314	39.0	37.0	39.0	35.0	39.0
12	36.87970876916949	39.0	35.0	39.0	33.0	39.0
13	36.72842526348875	39.0	35.0	39.0	33.0	39.0
14	37.859937064370826	40.0	35.0	41.0	33.0	41.0
15	37.9897865084003	40.0	35.0	41.0	33.0	41.0
16	38.06309685204437	40.0	35.0	41.0	34.0	41.0
17	38.00556331813834	40.0	35.0	41.0	34.0	41.0
18	37.92049047881839	40.0	36.0	41.0	34.0	41.0
19	37.874725311449176	40.0	36.0	41.0	34.0	41.0
20	37.77013953681617	40.0	35.0	41.0	34.0	41.0
21	37.646714621063026	40.0	35.0	41.0	33.0	41.0
22	37.61772566460404	40.0	35.0	41.0	33.0	41.0
23	37.53546496766962	39.0	35.0	41.0	33.0	41.0
24	37.51466676541756	39.0	35.0	41.0	33.0	41.0
25	37.41844696761001	39.0	35.0	41.0	33.0	41.0
26	37.38994709902697	39.0	35.0	41.0	33.0	41.0
27	37.332438403378724	39.0	35.0	41.0	33.0	41.0
28	37.282829176914326	39.0	35.0	41.0	33.0	41.0
29	37.20051067457999	39.0	35.0	41.0	33.0	41.0
30	37.15113312131999	39.0	35.0	41.0	33.0	41.0
31	36.98945503056009	39.0	35.0	41.0	32.0	41.0
32	36.867368671099015	39.0	35.0	41.0	32.0	41.0
33	36.77050443340321	39.0	35.0	41.0	32.0	41.0
34	36.61751160398243	39.0	35.0	41.0	31.0	41.0
35	36.50928318594462	39.0	35.0	41.0	31.0	41.0
36	36.42865007230191	39.0	35.0	41.0	31.0	41.0
37	36.16479822754192	39.0	35.0	41.0	30.0	41.0
38	36.241693860795564	39.0	35.0	41.0	30.0	41.0
39	36.15709069035002	39.0	35.0	41.0	30.0	41.0
40	36.056887287599025	38.0	35.0	41.0	30.0	41.0
41	35.99666986703855	38.0	35.0	41.0	30.0	41.0
42	35.998807763626495	38.0	35.0	41.0	30.0	41.0
43	35.892146413852345	38.0	35.0	41.0	30.0	41.0
44	35.770346371762784	38.0	35.0	40.0	30.0	41.0
45	35.72026973444741	38.0	35.0	40.0	30.0	41.0
46	35.56043057797271	38.0	35.0	40.0	29.0	41.0
47	35.53447640500992	37.0	35.0	40.0	29.0	41.0
48	35.40430948288554	37.0	35.0	40.0	29.0	41.0
49	35.38570698262135	37.0	35.0	40.0	29.0	41.0
50	35.26659444002374	37.0	35.0	40.0	28.0	41.0
51	34.71335611829514	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	1.0
11	7.0
12	11.0
13	16.0
14	29.0
15	52.0
16	123.0
17	239.0
18	551.0
19	1212.0
20	2037.0
21	3462.0
22	5485.0
23	8225.0
24	11882.0
25	17577.0
26	24453.0
27	29674.0
28	31381.0
29	33100.0
30	37740.0
31	45267.0
32	56880.0
33	77097.0
34	149971.0
35	296078.0
36	136153.0
37	185520.0
38	313559.0
39	746258.0
40	283.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.35374917695046	22.759114963198734	28.09319856245196	14.793937297398848
2	32.512014942695885	24.468393542775544	29.830160509337826	13.189431005190745
3	29.449277116377626	24.66100294175293	32.5242082692431	13.365511672626344
4	26.237825866651974	26.934561577653877	31.5465744429682	15.28103811272595
5	23.32339501952287	31.912012955635262	29.58385531308398	15.18073671175789
6	21.203562619054285	40.23124869599147	27.561298562180998	11.003890122773251
7	86.59068267274105	4.322760063333042	7.102748195161869	1.9838090687640393
8	87.92070363623063	3.0725376480247264	6.752709402319261	2.2540493134253943
9	83.02670880439466	4.521737088396198	9.14025306120237	3.311301046006776
10	44.27767185138954	22.761237505227324	19.91120548645502	13.049885156928113
11	39.00369683596725	22.92932476970419	21.525963205056527	16.54101518927204
12	34.22612569242289	22.628556048206093	25.945276350456076	17.200041908914947
13	24.169657042368648	30.642597341132245	27.249104242103467	17.93864137439564
14	19.070678843133308	33.7916368231236	29.293654141260138	17.844030192482947
15	18.807393310650735	24.133935111632162	40.01000755986246	17.04866401785464
16	21.564891529070245	20.998217967905358	38.16438952530025	19.272500977724146
17	22.05546970048674	20.634856836798196	28.557628822494973	28.75204464022009
18	24.55275329829483	23.777212569422932	31.663133612665884	20.00690051961635
19	27.748759667727334	25.31339107249791	26.658405311593686	20.279443948181072
20	28.44228898545201	25.263533915060382	25.404299095977738	20.88987800350987
21	25.54136111846223	26.311618072497005	28.373825714912797	19.773195094127967
22	23.97420253386358	23.421935162211888	28.531164787840634	24.072697516083903
23	21.94378786140794	27.186150548744852	27.789313768614015	23.08074782123319
24	22.195783276717158	25.654307450664447	32.71252742369462	19.43738184892378
25	21.608877825577625	25.226773293543946	31.336939547293397	21.827409333585027
26	20.078479862495406	29.53386267424038	27.715521562768984	22.67213590049523
27	20.462343846389373	28.08841155277046	30.220030835568025	21.229213765272142
28	18.462547971707867	27.1207581900768	34.68360124028711	19.733092597928216
29	20.05508673971222	25.97214682932865	33.196828289962724	20.775938140996402
30	21.624096903527303	26.188826758119628	31.312733536073733	20.87434280227934
31	27.324883508571006	24.367414734777086	26.916452229707822	21.39124952694409
32	27.10341657009853	26.659173039561473	26.658450472062377	19.578959918277615
33	26.011120313811066	25.636920670217485	26.87576264741506	21.47619636855639
34	21.164408492697103	27.197034221699965	28.842455898544294	22.796101387058634
35	21.276000010838512	26.28637337049739	29.569945888726412	22.867680729937685
36	27.32610284122573	25.87667759851079	26.831595709032907	19.96562385123058
37	22.283213944107597	29.21060403933296	27.358753860091063	21.14742815646838
38	22.628330245862625	30.51650931254025	24.577636716544898	22.277523725052227
39	22.119010479938364	28.55324825703171	26.192620237489873	23.13512102554005
40	23.207964861542518	25.382622071004903	27.378805108190935	24.030607959261644
41	20.34339117185094	24.997312952112743	27.93554336624327	26.723752509793048
42	22.61663368447103	27.089913589959203	25.526954928949035	24.766497796620733
43	22.418740510656516	26.473021587607242	26.381932922252638	24.726304979483597
44	21.59849091777814	28.413702408769083	27.957310712153493	22.030495961299284
45	21.182517840643158	30.333473932925862	25.757002356473258	22.72700586995772
46	23.20773905919905	28.343568200888214	27.475403350726136	20.973289389186597
47	23.11525041931495	26.34174010511551	27.629716672251508	22.91329280331803
48	23.02221985380653	25.509161704283834	28.955086107465654	22.51353233444398
49	22.216240969035272	24.38434991053711	29.859921258206786	23.539487862220827
50	21.056836256269403	28.13623648911678	28.0833535802768	22.723573674337022
51	20.963037962793194	29.631860891305074	26.672134094076483	22.732967051825252
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1405.0
1	2531.0
2	3657.0
3	13793.0
4	23929.0
5	16350.0
6	8771.0
7	8833.5
8	8896.0
9	9275.0
10	9654.0
11	9560.5
12	9467.0
13	9153.5
14	8840.0
15	8443.5
16	8047.0
17	7569.5
18	7092.0
19	6837.5
20	6583.0
21	7157.0
22	7731.0
23	8006.5
24	8282.0
25	9808.0
26	13306.5
27	15279.0
28	19067.5
29	22856.0
30	26787.5
31	30719.0
32	35398.5
33	40078.0
34	45325.0
35	50572.0
36	55868.5
37	61165.0
38	72079.0
39	82993.0
40	105497.0
41	128001.0
42	146775.5
43	165550.0
44	170919.0
45	176288.0
46	179575.5
47	182863.0
48	181615.5
49	180368.0
50	174613.0
51	168858.0
52	161564.0
53	154270.0
54	145135.0
55	136000.0
56	124860.5
57	113721.0
58	106764.5
59	99808.0
60	93148.5
61	86489.0
62	76091.5
63	65694.0
64	55687.0
65	45680.0
66	38050.5
67	30421.0
68	25027.0
69	19633.0
70	16076.5
71	12520.0
72	11103.5
73	9687.0
74	7529.0
75	4210.0
76	3049.0
77	2029.5
78	1010.0
79	1021.5
80	1033.0
81	703.5
82	374.0
83	273.0
84	172.0
85	102.0
86	32.0
87	30.5
88	29.0
89	28.0
90	27.0
91	23.5
92	20.0
93	12.5
94	5.0
95	3.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2214326.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.894664746341952
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.76097539154571	15.868178652560324
2	6.5026054076380015	2.5873430912541657
3	2.1599099127128105	1.2891205078716625
4	1.1197417350258465	0.8910754568330592
5	0.7668583152769594	0.7628194545189853
6	0.5592970647668118	0.6676216558289294
7	0.44614807276550106	0.6213176434427352
8	0.345536216294721	0.5499461744722379
9	0.31793568296228775	0.569270144109058
>10	5.043965227665956	25.576016340902175
>50	2.3634614275190606	33.444905005339486
>100	0.6073258966634132	15.383681981407301
>500	0.00415976610870934	0.5764955171575997
>1k	0.001848784937204151	0.5881274946550774
>5k	0.0	0.0
>10k+	2.3109811715051886E-4	0.6240808796472812
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13574	0.6130082020443242	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2778	0.1254557820302882	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02533502293700205	0.0
2	0.0	0.0	0.0	0.08625649520440982	0.0
3	0.0	0.0	0.0	0.13372014780118194	0.0
4	0.0	0.0	0.0	0.2103123027052024	0.0
5	0.0	0.0	0.0	0.40247009699565467	0.0
6	0.0	0.0	0.0	0.6714910090022879	0.0
7	0.0	0.0	0.0	0.8648681359474621	0.0
8	0.0	0.0	0.0	1.3526915187736583	0.0
9	0.0	0.0	0.0	1.5930355331599773	0.0
10	0.0	0.0	0.0	1.9791575404886181	0.0
11	0.0	0.0	0.0	2.224695008774679	0.0
12	0.0	0.0	0.0	2.4674325280017486	0.0
13	0.0	0.0	0.0	2.576314418021556	0.0
14	0.0	0.0	0.0	2.613029879069297	0.0
15	0.0	0.0	0.0	2.6699320696229916	0.0
16	9.032093738681658E-5	0.0	0.0	2.774162431367378	0.0
17	9.032093738681658E-5	0.0	0.0	2.8912635266893854	0.0
18	9.032093738681658E-5	0.0	0.0	3.066531305688503	0.0
19	9.032093738681658E-5	0.0	0.0	3.151613628706884	0.0
20	9.032093738681658E-5	0.0	0.0	3.247128019993443	0.0
21	9.032093738681658E-5	0.0	0.0	3.362648498911181	0.0
22	9.032093738681658E-5	0.0	0.0	3.489730057814432	0.0
23	9.032093738681658E-5	0.0	0.0	3.630721041075253	0.0
24	9.032093738681658E-5	0.0	0.0	3.739828733438527	0.0
25	9.032093738681658E-5	0.0	0.0	3.835614087537246	0.0
26	9.032093738681658E-5	0.0	0.0	3.9308123555429506	0.0
27	9.032093738681658E-5	0.0	0.0	4.029442819169354	0.0
28	9.032093738681658E-5	0.0	0.0	4.135660241536251	0.0
29	9.032093738681658E-5	0.0	0.0	4.249780745924493	0.0
30	9.032093738681658E-5	0.0	0.0	4.398765132144048	0.0
31	9.032093738681658E-5	0.0	0.0	4.527788591201115	0.0
32	9.032093738681658E-5	0.0	0.0	4.648547684487289	0.0
33	9.032093738681658E-5	0.0	0.0	4.771700282614213	0.0
34	9.032093738681658E-5	0.0	0.0	4.89078843855873	0.0
35	9.032093738681658E-5	0.0	0.0	5.033540680098595	0.0
36	9.032093738681658E-5	0.0	0.0	5.163918953216464	0.0
37	9.032093738681658E-5	0.0	0.0	5.29556171945775	0.0
38	9.032093738681658E-5	0.0	0.0	5.455836222850655	0.0
39	9.032093738681658E-5	0.0	0.0	5.6762193100744875	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	7925	0.0	42.58675	1
TCGATAG	225	0.0	40.0	1
TCTAACG	35	6.2509134E-6	38.571426	1
TATAGCG	170	0.0	38.38235	1
CACGACG	400	0.0	37.6875	26
ATAGGGC	970	0.0	37.577316	4
CGGCATA	30	1.1402636E-4	37.499996	44
TCACGAC	415	0.0	36.86747	25
CGACGGT	415	0.0	36.86747	28
GTAGGGC	1045	0.0	36.81818	4
GGGCGAT	2880	0.0	36.640625	7
GTAGGGA	1855	0.0	36.63073	4
TTTCGCG	80	0.0	36.562504	1
TACGGGT	420	0.0	36.42857	4
TTACGGG	785	0.0	36.401276	3
AGGGCGA	1640	0.0	36.35671	6
CTACGGG	390	0.0	36.346153	3
TAGGGTC	930	0.0	36.04839	5
CGCGAAA	25	0.0021075292	36.0	32
ACTACGG	100	0.0	36.0	2
>>END_MODULE