FastQCFastQC Report
Thu 26 May 2016
SRR1546853_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546853_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1855181
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161470.8703732951124445No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27150.1463469063126455No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG800.045.01
CTTACCG207.034408E-445.029
CGTTTTT87300.042.51
TCACGAC4500.041.025
ATATCGG556.184564E-1140.9090922
TGCAACG556.184564E-1140.9090921
CGGTCTA4400.040.39772831
ACGTAAG451.929402E-840.0000041
CACGACG4450.039.9438226
TAGGGAC16450.039.3920975
ACGTTAG800.039.3751
TAATGCG403.4597542E-739.3751
ACGACGG4650.038.7096827
ACGTAGG1950.038.0769272
CGTAAGG2850.037.8947372
ACGGTCT4700.037.8191530
CGGGATC3400.037.720596
TACGGGT900.037.5000044
TTACGGG6650.037.2180443
TAGACGG2300.037.1739122
Produced by FastQC (version 0.11.5)