##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546853_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1855181
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.982969316740522	33.0	31.0	34.0	30.0	34.0
2	32.369533754388385	34.0	31.0	34.0	31.0	34.0
3	32.414550386188736	34.0	31.0	34.0	31.0	34.0
4	36.02692405754479	37.0	35.0	37.0	35.0	37.0
5	35.98949320847939	37.0	35.0	37.0	35.0	37.0
6	36.03605200786338	37.0	35.0	37.0	35.0	37.0
7	36.26603495831404	37.0	36.0	37.0	35.0	37.0
8	36.138792926404484	37.0	36.0	37.0	35.0	37.0
9	37.96166250085571	39.0	39.0	39.0	35.0	39.0
10	37.69983953048247	39.0	38.0	39.0	35.0	39.0
11	37.47895003236881	39.0	37.0	39.0	35.0	39.0
12	36.957092057324864	39.0	35.0	39.0	33.0	39.0
13	36.83073996553436	39.0	35.0	39.0	33.0	39.0
14	37.9981554360464	40.0	36.0	41.0	33.0	41.0
15	38.118197631390146	40.0	36.0	41.0	33.0	41.0
16	38.2008763565388	40.0	36.0	41.0	34.0	41.0
17	38.14191768889397	40.0	36.0	41.0	34.0	41.0
18	38.04113992111821	40.0	36.0	41.0	34.0	41.0
19	37.96266455941496	40.0	36.0	41.0	34.0	41.0
20	37.8393601486863	40.0	35.0	41.0	34.0	41.0
21	37.69561406676761	40.0	35.0	41.0	33.0	41.0
22	37.66925760882631	40.0	35.0	41.0	33.0	41.0
23	37.581978793443874	39.0	35.0	41.0	33.0	41.0
24	37.562742934516905	39.0	35.0	41.0	33.0	41.0
25	37.44819993305236	39.0	35.0	41.0	33.0	41.0
26	37.38959702584276	39.0	35.0	41.0	33.0	41.0
27	37.33186788782334	39.0	35.0	41.0	33.0	41.0
28	37.262372781954966	39.0	35.0	41.0	33.0	41.0
29	37.18183023651061	39.0	35.0	41.0	33.0	41.0
30	37.11913446720293	39.0	35.0	41.0	33.0	41.0
31	36.93341889551478	39.0	35.0	41.0	32.0	41.0
32	36.781108689664244	39.0	35.0	41.0	32.0	41.0
33	36.64459748132393	39.0	35.0	41.0	31.0	41.0
34	36.46479400123223	39.0	35.0	41.0	31.0	41.0
35	36.32400396511176	39.0	35.0	41.0	30.0	41.0
36	36.22996408436697	39.0	35.0	41.0	30.0	41.0
37	35.96692128692564	39.0	35.0	41.0	30.0	41.0
38	36.05368856192469	39.0	35.0	41.0	30.0	41.0
39	35.95262349064593	38.0	35.0	41.0	30.0	41.0
40	35.83938278798672	38.0	35.0	41.0	29.0	41.0
41	35.77702067884481	38.0	35.0	41.0	29.0	41.0
42	35.777845935248365	38.0	35.0	41.0	29.0	41.0
43	35.67064291839988	38.0	35.0	41.0	29.0	41.0
44	35.52082842590561	38.0	35.0	40.0	29.0	41.0
45	35.46037610346376	38.0	35.0	40.0	28.0	41.0
46	35.29949961755753	37.0	35.0	40.0	28.0	41.0
47	35.2757208056788	37.0	35.0	40.0	28.0	41.0
48	35.13287167128167	37.0	35.0	40.0	27.0	41.0
49	35.10116101879008	37.0	35.0	40.0	27.0	41.0
50	34.99293114795807	37.0	34.0	40.0	27.0	41.0
51	34.445375949839935	36.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	3.0
11	6.0
12	5.0
13	22.0
14	25.0
15	49.0
16	88.0
17	265.0
18	530.0
19	1119.0
20	1923.0
21	3299.0
22	4915.0
23	7316.0
24	10959.0
25	16744.0
26	23341.0
27	27426.0
28	28506.0
29	28898.0
30	31773.0
31	37833.0
32	47061.0
33	62526.0
34	119380.0
35	240352.0
36	119316.0
37	156803.0
38	260980.0
39	623430.0
40	286.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.48542217713528	22.351619599381408	27.84391388225731	15.319044341226004
2	33.96520339524823	23.967526618696507	28.687928563304606	13.379341422750665
3	29.25935528662702	24.501814108704217	32.60377289331877	13.635057711349999
4	26.521239706529983	26.388099058798037	31.687204644722	15.403456589949982
5	23.259563352578535	30.933262037504694	30.24804587800328	15.559128731913491
6	21.186558077082505	39.29196126954728	28.58394949064269	10.937531162727518
7	84.52862550877785	4.65544871362956	8.521648291999542	2.29427748559305
8	84.92130956494272	3.89546895963251	8.552750378534494	2.6304710968902767
9	80.02561475133693	5.924165890012889	10.347453968103382	3.7027653905467988
10	43.676385215243144	28.427468802235467	15.76697907104482	12.129166911476561
11	36.29926136587211	23.07030958165268	24.93406303751494	15.696366014960264
12	32.28860148955816	22.23750674462492	29.0608840862428	16.413007679574125
13	23.391194713615544	31.17625719538956	29.200493105524473	16.23205498547042
14	17.971561804481613	33.695795720201964	30.736946960970386	17.59569551434604
15	16.7719483974879	24.79687965756441	41.42366701685711	17.007504928090576
16	19.85348060377936	22.16576172351916	39.09419080941428	18.886566863287193
17	20.332409613940637	21.591208620614378	29.848677837903686	28.227703927541302
18	23.264360728144585	23.893355958259598	32.61315203206587	20.22913128152994
19	26.635622076767714	25.537562103104765	27.72042188875371	20.106393931373812
20	27.60900418880961	24.881076293903398	26.571800810810377	20.93811870647662
21	24.70438194440327	26.094596699729028	29.69882723033494	19.502194125532764
22	23.93572379191033	23.549076882525206	28.525464631213882	23.989734694350577
23	21.270646907229	27.91905479842668	28.367474656111725	22.442823638232603
24	21.1555098936438	25.550175427626737	33.59116981038508	19.70314486834438
25	21.030508613445264	26.414781091440677	31.756146704822875	20.798563590291188
26	19.987699313436263	29.4792260162216	28.579798952231616	21.953275718110525
27	20.29872017878579	27.975114018524337	30.69732818522829	21.028837617461583
28	18.400414838228723	26.711194217707057	35.33046101701128	19.55792992705294
29	19.084337323420193	25.961671664382074	34.16103334391631	20.792957668281424
30	21.163056327118486	26.290588357685852	32.39457497678124	20.15178033841442
31	26.525983178999784	25.16110287891047	27.387085141557616	20.92582880053213
32	26.13459279714486	26.80897443430048	27.723817783817324	19.33261498473734
33	24.62093995141175	25.637606249740593	28.539425533142047	21.20202826570561
34	21.01719454867207	26.456987215802663	30.312567884211838	22.21325035131343
35	21.10182240978104	26.17065396853461	30.86270288451639	21.864820737167964
36	26.84471218711274	25.394287673278242	28.250019809387872	19.510980330221148
37	21.71631770700541	28.660384081122004	29.268842231566623	20.354455980305968
38	21.573312792660122	30.404418760218004	26.567865884784286	21.45440256233758
39	21.605169522542546	28.245599755495554	27.809038578985017	22.340192142976882
40	22.72274241704718	26.057080144740596	28.65930601919705	22.56087141901518
41	19.963442920124773	25.306533432586903	29.306250980362563	25.42377266692576
42	22.858146994821528	26.716476721139337	26.950901286720814	23.47447499731832
43	22.524486829047945	26.788760773207578	27.448211252702563	23.238541145041914
44	21.324927325150483	28.72204922322943	28.27562378010555	21.677399671514532
45	20.484146829878057	30.194034975563035	26.962706064799068	22.359112129759843
46	23.31680844079365	28.413831318884785	28.158654061247933	20.11070617907363
47	22.60313144647342	26.957154045885552	28.637421362120463	21.802293145520572
48	22.608413949905696	25.8149474363957	29.35767453418292	22.21896407951569
49	21.336893812517484	25.115878181158603	30.791766409854347	22.755461596469562
50	20.477031621173353	28.58766880428379	29.1136013143731	21.82169826016976
51	20.323246087578518	29.225665851472176	28.08178824599864	22.369299814950672
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1450.0
1	2344.0
2	3238.0
3	14561.5
4	25885.0
5	17449.5
6	9014.0
7	9240.5
8	9467.0
9	9781.0
10	10095.0
11	10220.0
12	10345.0
13	10025.0
14	9705.0
15	9479.5
16	9254.0
17	8674.0
18	8094.0
19	8225.0
20	8356.0
21	8641.0
22	8926.0
23	10082.5
24	11239.0
25	12364.0
26	15573.5
27	17658.0
28	19809.5
29	21961.0
30	27263.0
31	32565.0
32	35016.0
33	37467.0
34	42529.0
35	47591.0
36	52632.5
37	57674.0
38	66574.5
39	75475.0
40	88961.5
41	102448.0
42	122152.0
43	141856.0
44	146989.5
45	152123.0
46	154141.0
47	156159.0
48	151683.0
49	147207.0
50	140827.0
51	134447.0
52	124884.5
53	115322.0
54	103504.0
55	91686.0
56	87415.5
57	83145.0
58	77226.5
59	71308.0
60	68070.0
61	64832.0
62	58122.0
63	51412.0
64	45415.0
65	39418.0
66	33995.0
67	28572.0
68	22905.5
69	17239.0
70	14317.5
71	11396.0
72	9860.5
73	8325.0
74	6573.0
75	3529.5
76	2238.0
77	1662.0
78	1086.0
79	819.0
80	552.0
81	391.5
82	231.0
83	236.5
84	242.0
85	155.0
86	68.0
87	54.0
88	40.0
89	29.0
90	18.0
91	20.0
92	22.0
93	16.0
94	10.0
95	9.5
96	9.0
97	5.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1855181.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.99426593364339
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.81669304687918	17.555095728170464
2	6.235985835905849	2.7431186166669344
3	2.1786537407435267	1.4375366925372028
4	1.2531842527671369	1.1025147087685836
5	0.7838695893320268	0.8620318102532217
6	0.6167500055174269	0.8138978181555789
7	0.4634046990514217	0.7134572330085867
8	0.40233694564028094	0.7079284621873732
9	0.3561553332792259	0.7050037602445818
>10	5.4670356866382965	31.10684857474145
>50	2.1448869215501194	32.47983856413767
>100	0.27482064908597736	7.7471160186744115
>500	0.004978634887728393	0.7784455926985779
>1k	9.957269775456787E-4	0.3631067077491187
>5k	0.0	0.0
>10k+	2.4893174438641967E-4	0.8840597120061849
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16147	0.8703732951124445	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2715	0.1463469063126455	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.030940377246209398	0.0
2	0.0	0.0	0.0	0.09486944939604276	0.0
3	0.0	0.0	0.0	0.15675020388846156	0.0
4	0.0	0.0	0.0	0.2395992628212557	0.0
5	0.0	0.0	0.0	0.4256727510684941	0.0
6	0.0	0.0	0.0	0.7418144105615571	0.0
7	0.0	0.0	0.0	0.9561331212426173	0.0
8	0.0	0.0	0.0	1.4816344065619473	0.0
9	0.0	0.0	0.0	1.7660271423650846	0.0
10	0.0	0.0	0.0	2.1793560843928437	0.0
11	0.0	0.0	0.0	2.4664439750083686	0.0
12	0.0	0.0	0.0	2.727604476328725	0.0
13	0.0	0.0	0.0	2.8391299824653227	0.0
14	0.0	0.0	0.0	2.8867803195483352	0.0
15	0.0	0.0	0.0	2.9435941829934653	0.0
16	0.0	0.0	0.0	3.0526401467026667	0.0
17	0.0	0.0	0.0	3.181037321964811	0.0
18	0.0	0.0	0.0	3.382850514316393	0.0
19	0.0	0.0	0.0	3.4812236649685393	0.0
20	0.0	0.0	0.0	3.588922051271547	0.0
21	0.0	0.0	0.0	3.7199065751535834	0.0
22	0.0	0.0	0.0	3.8561196993716518	0.0
23	0.0	0.0	0.0	4.003975892379234	0.0
24	0.0	0.0	0.0	4.119544130734414	0.0
25	0.0	0.0	0.0	4.219857792851479	0.0
26	0.0	0.0	0.0	4.31596701346122	0.0
27	0.0	0.0	0.0	4.420916341855593	0.0
28	0.0	0.0	0.0	4.526135185731204	0.0
29	0.0	0.0	0.0	4.64418296651378	0.0
30	0.0	0.0	0.0	4.80648518931576	0.0
31	0.0	0.0	0.0	4.937038488427814	0.0
32	0.0	0.0	0.0	5.065597372978702	0.0
33	0.0	0.0	0.0	5.193455517278368	0.0
34	0.0	0.0	0.0	5.326380552625324	0.0
35	0.0	0.0	0.0	5.478548993332726	0.0
36	5.3903096247751565E-5	0.0	0.0	5.615247245417024	0.0
37	5.3903096247751565E-5	0.0	0.0	5.750166695325146	0.0
38	5.3903096247751565E-5	0.0	0.0	5.905784934192405	0.0
39	5.3903096247751565E-5	0.0	0.0	6.099836080684311	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	80	0.0	45.0	1
CTTACCG	20	7.034408E-4	45.0	29
CGTTTTT	8730	0.0	42.5	1
TCACGAC	450	0.0	41.0	25
ATATCGG	55	6.184564E-11	40.909092	2
TGCAACG	55	6.184564E-11	40.909092	1
CGGTCTA	440	0.0	40.397728	31
ACGTAAG	45	1.929402E-8	40.000004	1
CACGACG	445	0.0	39.94382	26
TAGGGAC	1645	0.0	39.392097	5
ACGTTAG	80	0.0	39.375	1
TAATGCG	40	3.4597542E-7	39.375	1
ACGACGG	465	0.0	38.70968	27
ACGTAGG	195	0.0	38.076927	2
CGTAAGG	285	0.0	37.894737	2
ACGGTCT	470	0.0	37.81915	30
CGGGATC	340	0.0	37.72059	6
TACGGGT	90	0.0	37.500004	4
TTACGGG	665	0.0	37.218044	3
TAGACGG	230	0.0	37.173912	2
>>END_MODULE