FastQCFastQC Report
Thu 26 May 2016
SRR1546852_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546852_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4814161
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT413910.8597759817338888No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC66190.13749020857424585No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCG60290.12523469821636626No Hit
GAATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC58230.12095565561683541TruSeq Adapter, Index 27 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT256000.043.136721
CGACGGT5150.039.75728228
TACGGGT8200.039.5121964
CACGACG5050.038.7623826
TACGGGA9650.038.7046624
TCACGAC5250.037.71428725
GGGCGAT70150.037.5908787
CTATCGA601.5643309E-1037.49999627
AGGGCGA37050.037.226726
TAGGGTA35450.037.0028235
CGGGTAT7350.036.7346956
CGAGGGA24300.036.6666644
CGGTCTA5300.036.50943431
GTTTTTT322100.036.359052
ATACCGG1550.036.290322
ACGGGTA8750.036.257145
CACGACC5900.036.22881327
CGTAGAT250.002107774836.016
CGTAATA250.002107774836.029
ACACGAC5950.035.9243726
Produced by FastQC (version 0.11.5)