##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546852_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4814161
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.868889096147804	31.0	31.0	34.0	30.0	34.0
2	32.263719472614234	33.0	31.0	34.0	30.0	34.0
3	32.31528629806938	34.0	31.0	34.0	30.0	34.0
4	35.94824061762787	37.0	35.0	37.0	35.0	37.0
5	35.90916257266843	37.0	35.0	37.0	35.0	37.0
6	35.96261300774943	37.0	35.0	37.0	35.0	37.0
7	36.20090271181209	37.0	35.0	37.0	35.0	37.0
8	36.04851084124523	37.0	35.0	37.0	35.0	37.0
9	37.83411543569066	39.0	38.0	39.0	35.0	39.0
10	37.50165771356629	39.0	37.0	39.0	35.0	39.0
11	37.396387241722906	39.0	37.0	39.0	35.0	39.0
12	37.004059274295145	39.0	35.0	39.0	33.0	39.0
13	36.90690465067537	39.0	35.0	39.0	33.0	39.0
14	38.103967233335155	40.0	36.0	41.0	33.0	41.0
15	38.18817505272466	40.0	36.0	41.0	33.0	41.0
16	38.258778632455375	40.0	36.0	41.0	34.0	41.0
17	38.19060745994992	40.0	36.0	41.0	34.0	41.0
18	38.07316124242625	40.0	36.0	41.0	34.0	41.0
19	38.00549192268393	40.0	36.0	41.0	34.0	41.0
20	37.847659436400235	40.0	35.0	41.0	34.0	41.0
21	37.73222831558812	40.0	35.0	41.0	33.0	41.0
22	37.69495120748974	40.0	35.0	41.0	33.0	41.0
23	37.597153481156944	40.0	35.0	41.0	33.0	41.0
24	37.588731037453876	40.0	35.0	41.0	33.0	41.0
25	37.49561325431368	39.0	35.0	41.0	33.0	41.0
26	37.455168616089075	39.0	35.0	41.0	33.0	41.0
27	37.388220294252726	39.0	35.0	41.0	33.0	41.0
28	37.31414549700353	39.0	35.0	41.0	33.0	41.0
29	37.21445024377041	39.0	35.0	41.0	33.0	41.0
30	37.16189695359171	39.0	35.0	41.0	33.0	41.0
31	36.99087463007573	39.0	35.0	41.0	32.0	41.0
32	36.795445146101265	39.0	35.0	41.0	31.0	41.0
33	36.632094356628286	39.0	35.0	41.0	31.0	41.0
34	36.42945094690435	39.0	35.0	41.0	30.0	41.0
35	36.275371347156856	39.0	35.0	41.0	30.0	41.0
36	36.19202120577189	39.0	35.0	41.0	30.0	41.0
37	35.92226558272563	39.0	35.0	41.0	28.0	41.0
38	36.023232708669276	39.0	35.0	41.0	29.0	41.0
39	35.918064019878024	39.0	35.0	41.0	29.0	41.0
40	35.80243992670789	39.0	35.0	41.0	28.0	41.0
41	35.738930833430786	39.0	35.0	41.0	28.0	41.0
42	35.731949346937085	39.0	35.0	41.0	28.0	41.0
43	35.622256089898116	38.0	35.0	41.0	27.0	41.0
44	35.48377401586694	38.0	35.0	41.0	27.0	41.0
45	35.44042004411568	38.0	35.0	40.0	27.0	41.0
46	35.26654488705301	38.0	35.0	40.0	26.0	41.0
47	35.242764627107405	38.0	35.0	40.0	26.0	41.0
48	35.11546581013805	38.0	35.0	40.0	26.0	41.0
49	35.08060698426995	38.0	35.0	40.0	26.0	41.0
50	34.95644599339324	37.0	34.0	40.0	26.0	41.0
51	34.416372447867865	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	10.0
12	16.0
13	28.0
14	53.0
15	119.0
16	278.0
17	712.0
18	1595.0
19	3042.0
20	5769.0
21	9238.0
22	14490.0
23	20969.0
24	31133.0
25	47813.0
26	67416.0
27	78278.0
28	78767.0
29	79321.0
30	86815.0
31	101822.0
32	125392.0
33	163587.0
34	287364.0
35	526502.0
36	308672.0
37	411704.0
38	694066.0
39	1668561.0
40	623.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.23056250092176	22.149882399030695	26.949347975690884	15.670207124356663
2	34.500985737701754	24.455206213502205	27.988926834810883	13.054881213985158
3	29.623978092963654	24.90849392033212	32.00136015392921	13.466167832775014
4	26.2191272788758	25.895914158251042	32.355565175323385	15.529393387549772
5	23.64663333860251	30.287167379736573	30.58213051038384	15.484068771277071
6	20.69253604106718	39.72812708174903	28.700265736854252	10.879071140329541
7	82.87705791310262	4.5937599511109	10.174690875523273	2.354491260263211
8	82.60284606185793	4.517464206120236	9.726762357968502	3.1529273740533394
9	76.9640026579917	5.739837118035728	12.669351938998302	4.626808284974267
10	37.71033000350425	24.692526901364538	24.285186972350946	13.311956122780272
11	33.991468087585766	24.97012459699624	25.427940611043127	15.610466704374865
12	29.46422024523068	23.2149901093877	29.987218956740335	17.33357068864128
13	22.961778802163035	28.794176181477937	31.057581996115214	17.18646302024382
14	18.572893594543267	32.113259195112086	31.660137664693806	17.65370954565084
15	17.91311092420881	24.583058190201783	40.134449180241376	17.369381705348037
16	19.87434570634426	22.626143994768768	38.22707632752623	19.27243397136074
17	20.727910844693394	22.558157070359716	30.840098617391483	25.873833467555407
18	22.98053596462603	23.439162088679627	32.84952455890029	20.730777387794053
19	25.522266496695895	25.453261741765594	28.942218592190834	20.08225316934768
20	26.69102674380853	24.556345332031896	28.133708033445497	20.618919890714082
21	24.267883853489735	26.770646017031837	29.88146844278785	19.08000168669058
22	23.069731153569645	23.518885222160208	30.07722425569066	23.33415936857949
23	21.093478178232925	26.934952944033242	29.358428187175296	22.61314069055854
24	20.7884613746819	25.412610837070055	33.42131681927547	20.37761096897258
25	20.320591687731255	25.986854199516802	31.815346433158343	21.8772076795936
26	19.74634832528451	28.223007082646383	30.17053231082218	21.86011228124693
27	20.557974691747948	27.72902692701802	30.887604299066858	20.825394082167172
28	18.653468382133457	27.134364637991958	34.50339529567042	19.708771684204162
29	19.945157629751062	25.592268310095985	32.97924602023073	21.48332803992222
30	21.156500582344464	26.629229890732773	31.443775976748594	20.770493550174162
31	24.48464021041257	25.752379282703675	27.759084085472008	22.00389642141175
32	25.240659795133563	26.218483345280724	27.79443811704677	20.74641874253894
33	23.670978182906637	26.747360547351867	27.966887688218158	21.614773581523345
34	20.77583196739785	26.497784349131653	30.12153104144211	22.604852642028384
35	21.255084738545303	26.775028919888637	29.749212791180017	22.22067355038604
36	24.332630337871958	25.999670555263936	28.471004604956086	21.196694501908016
37	21.345858603399428	28.35127450037504	29.02034227770945	21.28252461851608
38	21.20953162970661	28.53097351750388	27.985104777343338	22.274390075446167
39	21.322905486542723	26.936136950966116	28.810336837509176	22.930620724981985
40	22.13777229303299	25.68183739596578	29.108332687668735	23.072057623332498
41	19.689786029175178	25.631236678623754	28.920449482267003	25.75852780993407
42	22.416470907391755	26.79355758978563	27.636882106767928	23.153089396054682
43	22.206340834882752	26.82895316546331	27.736276372975478	23.22842962667846
44	21.473523631635917	28.262162399637237	28.738818664352937	21.52549530437391
45	20.702049640633124	29.048924620510196	27.293457780078402	22.955567958778282
46	22.18561032753163	27.99601010435671	28.57243453220613	21.24594503590553
47	21.348870550860262	26.983663404692948	29.515257175653243	22.152208868793544
48	22.432901600091895	25.673216994612353	29.215329524708462	22.678551880587293
49	21.54121974732461	24.66170117700675	30.40650281533999	23.390576260328643
50	20.05664122990486	27.97145753953804	29.5477238920759	22.4241773384812
51	20.434027860721734	28.223796420601637	27.94983383397439	23.392341884702237
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3400.0
1	5457.5
2	7515.0
3	39041.5
4	70568.0
5	48463.0
6	26358.0
7	26601.0
8	26844.0
9	27695.5
10	28547.0
11	28357.0
12	28167.0
13	27000.5
14	25834.0
15	25584.0
16	25334.0
17	23841.5
18	22349.0
19	21810.0
20	21271.0
21	21669.5
22	22068.0
23	23426.0
24	24784.0
25	30264.0
26	41350.0
27	46956.0
28	52715.5
29	58475.0
30	70439.0
31	82403.0
32	90180.0
33	97957.0
34	112193.5
35	126430.0
36	137957.5
37	149485.0
38	173138.0
39	196791.0
40	234272.5
41	271754.0
42	305301.0
43	338848.0
44	354295.5
45	369743.0
46	389529.5
47	409316.0
48	407314.5
49	405313.0
50	388550.0
51	371787.0
52	344947.0
53	318107.0
54	290454.0
55	262801.0
56	245176.5
57	227552.0
58	208855.0
59	190158.0
60	174415.0
61	158672.0
62	137351.5
63	116031.0
64	102376.0
65	88721.0
66	72777.5
67	56834.0
68	47510.5
69	38187.0
70	31914.5
71	25642.0
72	22155.0
73	18668.0
74	13954.0
75	7021.5
76	4803.0
77	3635.0
78	2467.0
79	1752.0
80	1037.0
81	836.5
82	636.0
83	497.0
84	358.0
85	218.5
86	79.0
87	65.5
88	52.0
89	45.0
90	38.0
91	24.5
92	11.0
93	13.0
94	15.0
95	11.0
96	7.0
97	5.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4814161.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.240747495448055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.26076326529606	16.447785905402387
2	7.462821921430179	3.0210618823032553
3	2.3320604858915384	1.4160794231712759
4	1.1955087010410506	0.9679195898553202
5	0.7547733904563851	0.7638588806255459
6	0.5002945657533456	0.6075801587254983
7	0.3680025403440886	0.5214052547751693
8	0.290844394351974	0.47095263572358603
9	0.24005722306582475	0.43730438728804266
>10	2.9177084687856394	14.268538452828869
>50	1.4068160291608225	20.98081402408967
>100	1.2630804454686615	37.33555301757413
>500	0.003686955266718919	0.47921021994295887
>1k	0.0031602473714713386	1.0055632116893505
>5k	3.1602473714713384E-4	0.3938372401584782
>10k+	1.0534157904904461E-4	0.8825357158464389
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41391	0.8597759817338888	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6619	0.13749020857424585	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCG	6029	0.12523469821636626	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	5823	0.12095565561683541	TruSeq Adapter, Index 27 (95% over 22bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0386025727016608E-4	0.0	0.0	0.05550292148517675	0.0
2	1.0386025727016608E-4	0.0	0.0	0.20934073455374674	0.0
3	1.454043601782325E-4	0.0	0.0	0.30925430204764653	0.0
4	1.454043601782325E-4	0.0	0.0	0.48012519730852377	0.0
5	1.454043601782325E-4	0.0	0.0	0.9223414007134368	0.0
6	1.454043601782325E-4	0.0	0.0	1.4367197108696614	0.0
7	1.454043601782325E-4	0.0	0.0	1.8126522980847546	0.0
8	1.454043601782325E-4	0.0	0.0	2.6160737042238513	0.0
9	1.6617641163226574E-4	0.0	0.0	2.9974901130228093	0.0
10	1.6617641163226574E-4	0.0	0.0	3.6936031013503703	0.0
11	1.6617641163226574E-4	0.0	0.0	4.065048925451392	0.0
12	1.6617641163226574E-4	0.0	0.0	4.47438297140457	0.0
13	1.8694846308629895E-4	0.0	0.0	4.634057730931724	0.0
14	1.8694846308629895E-4	0.0	0.0	4.703020941759115	0.0
15	1.8694846308629895E-4	0.0	0.0	4.799548664866006	0.0
16	2.2849256599436537E-4	0.0	0.0	4.978354483782325	0.0
17	2.2849256599436537E-4	0.0	0.0	5.165012138148267	0.0
18	2.2849256599436537E-4	0.0	0.0	5.44597490611552	0.0
19	2.2849256599436537E-4	0.0	0.0	5.585313827269175	0.0
20	2.2849256599436537E-4	0.0	0.0	5.7284124897360105	0.0
21	2.492646174483986E-4	0.0	0.0	5.901547538605377	0.0
22	2.492646174483986E-4	0.0	0.0	6.085359421922117	0.0
23	2.492646174483986E-4	0.0	0.0	6.282195381500536	0.0
24	2.700366689024318E-4	0.0	0.0	6.438608928949406	0.0
25	2.700366689024318E-4	0.0	0.0	6.5763484021411	0.0
26	2.700366689024318E-4	0.0	0.0	6.712696147885374	0.0
27	2.700366689024318E-4	0.0	0.0	6.855462457528945	0.0
28	3.115807718104982E-4	0.0	0.0	7.003089427212759	0.0
29	3.115807718104982E-4	0.0	0.0	7.1593575703014505	0.0
30	3.115807718104982E-4	0.0	0.0	7.372042605139296	0.0
31	3.115807718104982E-4	0.0	0.0	7.5405247144829595	0.0
32	3.115807718104982E-4	0.0	0.0	7.705163994307627	0.0
33	3.115807718104982E-4	0.0	0.0	7.873396839033842	0.0
34	3.115807718104982E-4	0.0	0.0	8.037454501417796	0.0
35	3.115807718104982E-4	0.0	0.0	8.217236606752454	0.0
36	3.115807718104982E-4	0.0	0.0	8.382623680429466	0.0
37	3.115807718104982E-4	0.0	0.0	8.548717003855916	0.0
38	3.323528232645315E-4	0.0	0.0	8.737971164653613	0.0
39	3.323528232645315E-4	2.0772051454033217E-5	0.0	8.976704352014817	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	25600	0.0	43.13672	1
CGACGGT	515	0.0	39.757282	28
TACGGGT	820	0.0	39.512196	4
CACGACG	505	0.0	38.76238	26
TACGGGA	965	0.0	38.704662	4
TCACGAC	525	0.0	37.714287	25
GGGCGAT	7015	0.0	37.590878	7
CTATCGA	60	1.5643309E-10	37.499996	27
AGGGCGA	3705	0.0	37.22672	6
TAGGGTA	3545	0.0	37.002823	5
CGGGTAT	735	0.0	36.734695	6
CGAGGGA	2430	0.0	36.666664	4
CGGTCTA	530	0.0	36.509434	31
GTTTTTT	32210	0.0	36.35905	2
ATACCGG	155	0.0	36.29032	2
ACGGGTA	875	0.0	36.25714	5
CACGACC	590	0.0	36.228813	27
CGTAGAT	25	0.0021077748	36.0	16
CGTAATA	25	0.0021077748	36.0	29
ACACGAC	595	0.0	35.92437	26
>>END_MODULE