FastQCFastQC Report
Thu 26 May 2016
SRR1546851_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546851_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1379469
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250441.8154811742779287No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTC16690.12098858328820727No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCG14610.1059103176657105No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTC207.033777E-445.00000438
GTCGTTA207.033777E-445.00000434
ATCGGAT253.8912127E-545.00000412
TCGCGAT207.033777E-445.00000413
CGTTTTT159950.044.1137851
GCGATAA1450.041.8965539
CGACGGT1300.041.53846428
AATAGCG650.041.5384641
ACGGGTA2300.041.086965
TGTAGCG501.0822987E-940.5000041
GACGGTC1350.040.00000429
TTACGCG451.9288564E-840.01
GGCGATA4500.039.5000048
AGGGCGA9200.038.885876
TAGGGCG4750.038.8421065
GGGCGAT18050.038.642667
ATACGCG356.249198E-638.571431
ACGGTCT1400.038.5714330
GTTTTTT187100.038.3979152
CGAGGGT4450.037.921354
Produced by FastQC (version 0.11.5)