##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546851_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1379469
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.948762168631553	33.0	31.0	34.0	30.0	34.0
2	32.35467415360548	34.0	31.0	34.0	31.0	34.0
3	32.34412371716943	34.0	31.0	34.0	30.0	34.0
4	36.00485259183063	37.0	35.0	37.0	35.0	37.0
5	35.97221902050717	37.0	35.0	37.0	35.0	37.0
6	36.03216237552275	37.0	35.0	37.0	35.0	37.0
7	36.26332161143164	37.0	36.0	37.0	35.0	37.0
8	36.13812198751839	37.0	37.0	37.0	35.0	37.0
9	37.978454028325395	39.0	39.0	39.0	35.0	39.0
10	37.629403777830454	39.0	37.0	39.0	35.0	39.0
11	37.500841990650024	39.0	37.0	39.0	35.0	39.0
12	37.20748853363142	39.0	37.0	39.0	34.0	39.0
13	37.136016104747554	39.0	37.0	39.0	33.0	39.0
14	38.40237874138527	40.0	38.0	41.0	34.0	41.0
15	38.49020093963692	40.0	38.0	41.0	34.0	41.0
16	38.53205182573875	40.0	37.0	41.0	34.0	41.0
17	38.46438810875779	40.0	37.0	41.0	34.0	41.0
18	38.241179033381684	40.0	37.0	41.0	34.0	41.0
19	38.06080310612272	40.0	37.0	41.0	34.0	41.0
20	37.79773594042345	40.0	35.0	41.0	34.0	41.0
21	37.7022970432826	40.0	35.0	41.0	34.0	41.0
22	37.67598981927104	40.0	35.0	41.0	33.0	41.0
23	37.57794702164384	39.0	35.0	41.0	33.0	41.0
24	37.55471851850241	39.0	35.0	41.0	33.0	41.0
25	37.473913513098154	39.0	35.0	41.0	33.0	41.0
26	37.4311456074765	39.0	35.0	41.0	33.0	41.0
27	37.35690399711773	39.0	35.0	41.0	33.0	41.0
28	37.28931929604797	39.0	35.0	41.0	33.0	41.0
29	37.164869235916136	39.0	35.0	41.0	33.0	41.0
30	37.05745471627126	39.0	35.0	41.0	33.0	41.0
31	36.77978555516652	39.0	35.0	41.0	32.0	41.0
32	36.38487925426378	39.0	35.0	41.0	30.0	41.0
33	35.99213175504487	39.0	35.0	41.0	28.0	41.0
34	35.59531094935805	39.0	35.0	41.0	24.0	41.0
35	35.32435814070487	39.0	35.0	41.0	23.0	41.0
36	35.20278020020747	39.0	35.0	41.0	22.0	41.0
37	34.934735032102935	39.0	35.0	41.0	20.0	41.0
38	35.01987866345674	39.0	35.0	41.0	21.0	41.0
39	34.91232785948796	39.0	35.0	41.0	20.0	41.0
40	34.83327425262909	39.0	35.0	41.0	19.0	41.0
41	34.73265510134697	39.0	35.0	41.0	18.0	41.0
42	34.72619609429425	39.0	35.0	41.0	18.0	41.0
43	34.628753527625484	39.0	35.0	41.0	18.0	41.0
44	34.50658043058597	38.0	34.0	41.0	18.0	41.0
45	34.47239771245312	38.0	34.0	41.0	18.0	41.0
46	34.31581354854658	38.0	34.0	40.0	18.0	41.0
47	34.299563817671874	38.0	34.0	40.0	18.0	41.0
48	34.17660491102011	38.0	34.0	40.0	18.0	41.0
49	34.14220616773556	38.0	34.0	40.0	18.0	41.0
50	34.03792546262366	38.0	34.0	40.0	18.0	41.0
51	33.52693826392619	37.0	33.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	5.0
14	10.0
15	29.0
16	69.0
17	190.0
18	480.0
19	990.0
20	1791.0
21	3096.0
22	4839.0
23	7433.0
24	11952.0
25	20670.0
26	31403.0
27	35835.0
28	33098.0
29	28378.0
30	26310.0
31	28256.0
32	32879.0
33	41859.0
34	72084.0
35	131806.0
36	81103.0
37	103220.0
38	184953.0
39	496564.0
40	165.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.053810560440287	22.023184283227824	26.51991454682925	21.40309060950264
2	37.44520536525286	23.868749497089098	27.016844887416823	11.669200250241216
3	26.548548753179666	23.546016619438348	37.57351560636738	12.331919021014608
4	23.092871242485334	25.07269101371615	38.56860864579052	13.265829098008004
5	22.024851591445692	29.243571258216022	35.717439101567344	13.01413804877094
6	18.7301780612685	38.457841386794485	33.091863608388444	9.720116943548568
7	80.09538452839462	3.1930402205486312	15.196354539319115	1.5152207117376324
8	80.99167143299341	2.937289638259359	14.386260220418146	1.6847787083290746
9	76.11798452882958	4.1142642567538665	16.40218083914898	3.365570375267585
10	33.36928919751006	25.082477388038445	28.21868414585612	13.32954926859538
11	29.51367518951133	22.5039489832682	31.606002019617698	16.376373807602782
12	27.531608176769467	21.84673957877995	33.86636452142092	16.755287723029657
13	22.08842677870978	26.3226647354888	35.0311605407588	16.55774794504262
14	17.568571675043078	29.64669738863287	35.83132350201418	16.95340743430987
15	16.912087187171295	24.81875272296804	42.571380726931885	15.69777936292878
16	19.058637780189333	22.646395098403808	41.14372994246337	17.151237178943493
17	19.14127827446648	22.348744335682788	36.046333770458055	22.463643619392677
18	20.43097742682148	23.90898236930297	37.52900572611635	18.131034477759194
19	22.695326970015273	24.95278980535264	34.251440228087766	18.100442996544324
20	23.748775797063942	24.76960337637163	33.62866436288166	17.852956463682766
21	21.90850247450287	25.421303414574737	35.72657305093482	16.943621059987574
22	20.829826549201176	22.78144706405146	36.083521992882766	20.305204393864596
23	18.824272238085815	26.54282191190958	35.36302736777702	19.269878482227583
24	18.52654898370315	25.763609040870072	37.82027722261247	17.88956475281431
25	18.536335358025443	26.53992224544372	36.555877660172136	18.3678647363587
26	18.201423881218062	28.149309625660308	34.48442842861999	19.16483806450163
27	18.786939032337806	26.442348468867365	36.450329800814664	18.320382697980165
28	16.898241279796792	26.489830507245905	39.13911802294941	17.472810190007895
29	17.750453254114447	25.15881110775233	38.71859389373738	18.37214174439585
30	18.97693967751359	26.00493378249167	36.64866698707981	18.369459552914925
31	22.061894830547114	25.247323426622852	33.510430462736025	19.18035128009401
32	22.657123864327506	26.175289187361223	33.03974210366453	18.127844844646745
33	21.354521196199407	26.901945603706935	32.53722990513016	19.206303294963497
34	18.967950711469413	27.67905621655869	33.46142609946291	19.891566972508986
35	19.972395175244966	27.573000915569683	32.35252115125458	20.10208275793077
36	22.86416004999025	27.761551727512547	30.641500461409425	18.73278776108778
37	20.054310752905646	29.5721034687985	31.28268920867377	19.09089656962208
38	20.691729933764368	29.852428724385977	29.442053427804467	20.013787914045185
39	19.98094919131927	29.23508973380337	30.057725110169205	20.72623596470816
40	21.115081237780625	27.422073276021425	30.5262387193913	20.936606766806648
41	19.005138933894127	27.33646062361677	31.343002271163762	22.31539817132534
42	21.10145280539106	28.474217253160454	29.429004928708075	20.99532501274041
43	20.71833437358868	29.071403561805305	29.51469007277438	20.695571991831642
44	20.3672572562341	29.522664155555507	30.350736406544836	19.759342181665556
45	19.810376311464772	29.929849819024568	29.359702900173907	20.900070969336753
46	21.364307570521703	29.23313245893891	30.168347385841944	19.234212584697445
47	20.835118440501383	27.99019042834598	31.155321359160666	20.019369771991975
48	21.288481292439336	27.323049666212146	30.851798771846266	20.53667026950225
49	20.207703108949893	26.392401713992847	32.0443590975948	21.35553607946246
50	19.1493973405709	28.448482713275908	31.72575824465791	20.676361701495285
51	19.14214817440624	29.19239214509351	30.545883959697534	21.119575720802715
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	872.0
1	1427.5
2	1983.0
3	22153.0
4	42323.0
5	29225.0
6	16127.0
7	16131.0
8	16135.0
9	16517.5
10	16900.0
11	16802.0
12	16704.0
13	16001.5
14	15299.0
15	14523.5
16	13748.0
17	12873.5
18	11999.0
19	11205.0
20	10411.0
21	9908.0
22	9405.0
23	9779.5
24	10154.0
25	11295.0
26	14128.5
27	15821.0
28	18149.0
29	20477.0
30	22982.0
31	25487.0
32	29396.5
33	33306.0
34	39066.5
35	44827.0
36	48157.0
37	51487.0
38	55602.5
39	59718.0
40	68691.5
41	77665.0
42	85760.0
43	93855.0
44	98920.5
45	103986.0
46	108079.5
47	112173.0
48	110633.5
49	109094.0
50	103067.5
51	97041.0
52	89672.5
53	82304.0
54	74049.5
55	65795.0
56	60265.0
57	54735.0
58	49218.5
59	43702.0
60	38599.5
61	33497.0
62	28379.0
63	23261.0
64	19224.5
65	15188.0
66	12099.5
67	9011.0
68	7051.0
69	5091.0
70	4084.0
71	3077.0
72	2524.5
73	1972.0
74	1642.5
75	941.5
76	570.0
77	397.0
78	224.0
79	179.0
80	134.0
81	118.0
82	102.0
83	65.5
84	29.0
85	20.0
86	11.0
87	9.5
88	8.0
89	5.5
90	3.0
91	4.5
92	6.0
93	4.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1379469.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.7165873138294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.2581193094848	22.36414473534895
2	6.266021303136125	3.7240953832991948
3	2.9827674698607782	2.659130098649034
4	1.8552275547980321	2.2052412647671176
5	1.463553825749574	2.1745912525688134
6	1.1801290225153185	2.104164428349636
7	0.9397250952987127	1.9547795991808596
8	0.8136692565001991	1.934357880429345
9	0.7248042395821777	1.9384837623879756
>10	8.276456096901008	50.993018729974914
>50	0.2113531964560212	3.7938924829091976
>100	0.024713710388994267	1.560908891380391
>500	0.002718508043682684	0.5240729107341969
>1k	4.942741897604881E-4	0.22986942413089073
>5k	0.0	0.0
>10k+	2.4713709488024404E-4	1.8392491558894657
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25044	1.8154811742779287	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTC	1669	0.12098858328820727	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCG	1461	0.1059103176657105	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4498332329323819E-4	0.0	0.0	0.021530023509045874	0.0
2	1.4498332329323819E-4	0.0	0.0	0.08865730219381515	0.0
3	1.4498332329323819E-4	0.0	0.0	0.15955414728420864	0.0
4	1.4498332329323819E-4	0.0	0.0	0.24364447479428678	0.0
5	1.4498332329323819E-4	0.0	0.0	0.5074416315263337	0.0
6	1.4498332329323819E-4	0.0	0.0	0.8199531848849086	0.0
7	1.4498332329323819E-4	0.0	0.0	1.0629452347243757	0.0
8	1.4498332329323819E-4	0.0	0.0	1.5395779100508964	0.0
9	1.4498332329323819E-4	0.0	0.0	1.839765880929546	0.0
10	1.4498332329323819E-4	0.0	0.0	2.315383672993014	0.0
11	1.4498332329323819E-4	0.0	0.0	2.537063174308375	0.0
12	1.4498332329323819E-4	0.0	0.0	2.790783990071542	0.0
13	1.4498332329323819E-4	0.0	0.0	2.896839291060546	0.0
14	1.4498332329323819E-4	0.0	0.0	2.9490332874461114	0.0
15	1.4498332329323819E-4	0.0	0.0	3.0005748588768575	0.0
16	2.174749849398573E-4	0.0	0.0	3.082925386507417	0.0
17	2.174749849398573E-4	0.0	0.0	3.175642221753443	0.0
18	2.174749849398573E-4	0.0	0.0	3.32685982794829	0.0
19	2.174749849398573E-4	0.0	0.0	3.4057307558198118	0.0
20	2.174749849398573E-4	0.0	0.0	3.480107200669243	0.0
21	2.174749849398573E-4	0.0	0.0	3.5648499531341407	0.0
22	2.174749849398573E-4	0.0	0.0	3.661843796417317	0.0
23	2.174749849398573E-4	0.0	0.0	3.7623897311211776	0.0
24	2.174749849398573E-4	0.0	0.0	3.849162250112181	0.0
25	2.174749849398573E-4	0.0	0.0	3.9265108530891233	0.0
26	2.174749849398573E-4	0.0	0.0	4.005889222592171	0.0
27	2.174749849398573E-4	0.0	0.0	4.091284400011888	0.0
28	2.174749849398573E-4	0.0	0.0	4.171677652777989	0.0
29	2.174749849398573E-4	0.0	0.0	4.260044988325218	0.0
30	2.174749849398573E-4	0.0	0.0	4.385745529620455	0.0
31	2.174749849398573E-4	0.0	0.0	4.486363955985962	0.0
32	2.174749849398573E-4	0.0	0.0	4.580965574434801	0.0
33	2.174749849398573E-4	0.0	0.0	4.684193700619586	0.0
34	2.174749849398573E-4	0.0	0.0	4.787856776774252	0.0
35	2.174749849398573E-4	0.0	0.0	4.903335993777316	0.0
36	2.174749849398573E-4	0.0	0.0	5.005694220022342	0.0
37	2.174749849398573E-4	0.0	0.0	5.111604537688052	0.0
38	2.174749849398573E-4	0.0	0.0	5.233318037592726	0.0
39	2.174749849398573E-4	0.0	0.0	5.388957635148017	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGTC	20	7.033777E-4	45.000004	38
GTCGTTA	20	7.033777E-4	45.000004	34
ATCGGAT	25	3.8912127E-5	45.000004	12
TCGCGAT	20	7.033777E-4	45.000004	13
CGTTTTT	15995	0.0	44.113785	1
GCGATAA	145	0.0	41.896553	9
CGACGGT	130	0.0	41.538464	28
AATAGCG	65	0.0	41.538464	1
ACGGGTA	230	0.0	41.08696	5
TGTAGCG	50	1.0822987E-9	40.500004	1
GACGGTC	135	0.0	40.000004	29
TTACGCG	45	1.9288564E-8	40.0	1
GGCGATA	450	0.0	39.500004	8
AGGGCGA	920	0.0	38.88587	6
TAGGGCG	475	0.0	38.842106	5
GGGCGAT	1805	0.0	38.64266	7
ATACGCG	35	6.249198E-6	38.57143	1
ACGGTCT	140	0.0	38.57143	30
GTTTTTT	18710	0.0	38.397915	2
CGAGGGT	445	0.0	37.92135	4
>>END_MODULE