FastQCFastQC Report
Thu 26 May 2016
SRR1546850_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546850_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5808333
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT671671.156390310266302No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCG71190.12256528680432062No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC67510.11622956190700498No Hit
GAATCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC59920.1031621293062915No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT358450.043.52491
ACGTAAG2400.039.3749961
CGACGGT7100.038.6619728
ACGGGTA7650.038.5294155
CACGACG7150.038.07692326
TAGGGAC48800.037.3463135
GGGCGAT76850.037.3292127
TAGGGAT76900.037.246425
TGGGCGA22750.036.9890146
TAAGGGA68600.036.865894
CGGGATA12100.036.632236
TACGGGT10150.036.354684
TTAGGGA68250.036.2967034
GCGGGAT28650.036.2827225
GTTTTTT450600.036.2816242
TACGGGA25100.036.1254964
ATAGGGA61950.036.1016964
CTACGGG12800.036.0351563
CGGGTAT6450.035.9302336
AGGGATT92250.035.8780486
Produced by FastQC (version 0.11.5)