FastQCFastQC Report
Thu 26 May 2016
SRR1546849_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546849_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1997773
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC76980.38532906391266675No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59830.29948347484924465No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAG406.8193913E-945.0000041
CGAACAA207.034538E-445.00000438
CGTATTA207.034538E-445.00000413
GCACGTC302.166309E-645.0000049
ATTCGAC406.8193913E-945.00000440
CGCGACA302.166309E-645.00000428
GCGTTCC207.034538E-445.00000445
TCGCTAA207.034538E-445.00000415
AATCTCG600.045.00000418
ACGTCGT207.034538E-445.00000437
ACGTCGA207.034538E-445.00000420
CTATTCG207.034538E-445.0000041
CGTAAGT207.034538E-445.00000432
ACCGTTG207.034538E-445.00000427
ACCCGGT207.034538E-445.00000420
TCGAACA207.034538E-445.00000434
ATCGATC207.034538E-445.00000433
ATCGAAC207.034538E-445.00000433
TGCGTAT207.034538E-445.00000413
CTTAACG1000.045.01