##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546849_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1997773
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06756473333056	33.0	31.0	34.0	31.0	34.0
2	32.45475737233409	34.0	31.0	34.0	31.0	34.0
3	32.51241106972614	34.0	31.0	34.0	31.0	34.0
4	36.066331359969325	37.0	35.0	37.0	35.0	37.0
5	36.01986662148302	37.0	35.0	37.0	35.0	37.0
6	36.07970274901103	37.0	35.0	37.0	35.0	37.0
7	36.32134832135583	37.0	37.0	37.0	35.0	37.0
8	36.2234678314303	37.0	37.0	37.0	35.0	37.0
9	38.10508300993156	39.0	39.0	39.0	37.0	39.0
10	37.79898216664256	39.0	38.0	39.0	35.0	39.0
11	37.52075536109458	39.0	37.0	39.0	35.0	39.0
12	36.49033899246811	38.0	35.0	39.0	33.0	39.0
13	36.15922629848336	38.0	35.0	39.0	33.0	39.0
14	37.15589108472284	39.0	35.0	41.0	33.0	41.0
15	37.42102431056982	39.0	35.0	41.0	33.0	41.0
16	37.56900158326296	39.0	35.0	41.0	34.0	41.0
17	37.52950009835952	39.0	35.0	41.0	34.0	41.0
18	37.51781308487	39.0	35.0	41.0	34.0	41.0
19	37.47403583890662	38.0	35.0	41.0	34.0	41.0
20	37.338390297596376	38.0	35.0	41.0	34.0	41.0
21	37.16925746819083	38.0	35.0	41.0	33.0	41.0
22	37.10869453136067	38.0	35.0	41.0	33.0	41.0
23	37.075555130637966	38.0	35.0	41.0	33.0	41.0
24	37.05840403289062	38.0	35.0	41.0	33.0	41.0
25	36.956692777407646	38.0	35.0	41.0	33.0	41.0
26	36.91145941005309	38.0	35.0	41.0	33.0	41.0
27	36.8278598219117	38.0	35.0	41.0	33.0	41.0
28	36.83911885884933	38.0	35.0	41.0	33.0	41.0
29	36.798733890186725	38.0	35.0	41.0	33.0	41.0
30	36.80450581722748	38.0	35.0	41.0	33.0	41.0
31	36.61997283975707	38.0	35.0	41.0	33.0	41.0
32	36.45022482534302	38.0	35.0	41.0	32.0	41.0
33	36.37529539141834	38.0	35.0	41.0	32.0	41.0
34	36.28397820973654	37.0	35.0	41.0	32.0	41.0
35	36.20545377277599	37.0	35.0	41.0	32.0	41.0
36	36.09105939463593	37.0	35.0	41.0	31.0	41.0
37	35.812855614727	37.0	35.0	40.0	30.0	41.0
38	35.919212543166815	37.0	35.0	40.0	31.0	41.0
39	35.888254070907955	37.0	35.0	40.0	31.0	41.0
40	35.77441931590826	37.0	35.0	40.0	30.0	41.0
41	35.76669921958101	36.0	35.0	40.0	31.0	41.0
42	35.76319681965869	36.0	35.0	40.0	31.0	41.0
43	35.63516075149679	36.0	35.0	40.0	31.0	41.0
44	35.5298670069122	36.0	35.0	40.0	30.0	41.0
45	35.47618823560034	36.0	35.0	40.0	30.0	41.0
46	35.36108857212506	35.0	35.0	40.0	30.0	41.0
47	35.344474071879034	35.0	35.0	40.0	30.0	41.0
48	35.25519315758097	35.0	35.0	40.0	30.0	41.0
49	35.265218320599985	36.0	35.0	40.0	30.0	41.0
50	35.142665357876	35.0	35.0	40.0	30.0	41.0
51	34.59311593459317	35.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	4.0
12	2.0
13	4.0
14	6.0
15	29.0
16	56.0
17	177.0
18	470.0
19	892.0
20	1638.0
21	2745.0
22	4348.0
23	6527.0
24	9788.0
25	14815.0
26	20600.0
27	24847.0
28	26495.0
29	28070.0
30	32772.0
31	40448.0
32	52962.0
33	74706.0
34	165714.0
35	431689.0
36	106081.0
37	131117.0
38	225826.0
39	594661.0
40	282.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.79211401896011	23.6416750051182	30.20558391769235	13.360627058229339
2	29.43247305875092	25.915056415318453	32.17332499738459	12.479145528546036
3	27.99977775252744	25.155660828332348	33.23841097061578	13.606150448524431
4	25.275043761228126	27.206244152864212	32.28875352705237	15.229958558855285
5	22.81760740584641	32.089331470592505	30.28642393304945	14.806637190511635
6	20.894866433774006	39.997086756102924	27.738536860794493	11.369509949328577
7	87.81017663167937	4.570739518453798	5.9086292586795395	1.710454591187287
8	89.54335652749337	3.0353298397765913	5.30125294515443	2.1200606875756156
9	86.33488389321509	4.132301317517055	6.915400298232082	2.6174144910357686
10	55.60862019859113	21.437370512065186	11.938093066629692	11.015916222713992
11	50.252656332826604	20.36357484058499	17.966655871312707	11.417112955275698
12	43.97051116418132	21.818745172749857	20.9874194916039	13.223324171464926
13	21.249761609552237	43.28384656314807	22.573535631926152	12.892856195373547
14	14.557710010096242	44.71048512518689	27.05217259418362	13.679632270533236
15	12.647182637867266	23.231318072673922	50.69625027468086	13.425249014777954
16	14.207019516231323	17.782550870394186	48.911112523795246	19.099317089579245
17	15.047755675945165	17.864542167703735	29.01355659526883	38.07414556108227
18	21.84722688713883	23.078547963156975	33.751031773880214	21.32319337582398
19	28.70010756977895	24.250853325177584	25.37740774352241	21.671631361521055
20	30.616341296033134	24.243845521988735	23.811013563603073	21.32879961837506
21	22.4779291741354	27.723870529834972	28.042325129031177	21.755875166998454
22	22.406199302923806	24.72488115516628	25.76619065329244	27.102728888617477
23	19.14276546935012	30.94911183602942	24.854625625634146	25.053497068986317
24	19.996466064963336	24.560197780228286	37.54690848259537	17.896427672213008
25	16.558988433620836	25.109909884656567	36.38521493683216	21.945886744890437
26	15.849097970590254	34.71420426645069	27.243235342553934	22.193462420405123
27	18.10135585974983	32.75477243911095	29.76359175942412	19.380279941715102
28	14.346374688215327	28.06500037792081	40.493689723507124	17.09493521035673
29	14.583989272054431	24.958291057092072	39.36157911834828	21.096140552505215
30	18.51656819868924	28.632932770640107	33.03593551419506	19.814563516475594
31	29.415203829464108	25.762536584486828	24.747105902422348	20.075153683626716
32	29.542745847501195	27.197734677563467	26.16558537931987	17.093934095615467
33	25.877814946943424	26.93954718579138	25.88216979606792	21.30046807119728
34	18.294771227762112	27.468486159338422	30.474082891299464	23.762659721600002
35	19.099216978105122	25.43241899855489	31.315820165754566	24.15254385758542
36	30.49820975656393	22.89379223765663	27.473992290415378	19.134005715364058
37	20.36262378158079	29.825210371748945	30.334677663578397	19.47748818309187
38	19.943607206624574	32.17612811866013	24.078961924102487	23.801302750612805
39	19.76315627451167	30.70013459987696	26.791832705717816	22.74487641989355
40	22.669041978242774	25.82130201979905	25.0818286161641	26.427827385794085
41	17.460442202392365	23.507275351103456	27.804159932084378	31.228122514419805
42	22.77035479005873	26.308995066006	24.500831676071304	26.419818467863966
43	22.773958803127282	26.589056914874714	25.986035450474105	24.650948831523902
44	20.22006504242474	31.154941026833377	27.15688919611988	21.468104734622003
45	17.073911800790178	36.18389076236389	23.340489635208804	23.401707801637123
46	22.90044965068604	31.085413608052566	26.458711775562087	19.555424965699306
47	22.456805653094722	26.765503388022566	27.891707416207943	22.88598354267477
48	23.616897415271907	23.68382193572543	30.001706900633856	22.69757374836881
49	21.04803698918746	22.65717876855879	31.754008088005996	24.540776154247755
50	18.87366582689825	31.653446112245987	27.0539746007179	22.418913460137862
51	18.131889859358395	33.13034063429629	24.029506855884026	24.708262650461286
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1619.0
1	2275.5
2	2932.0
3	6900.5
4	10869.0
5	8086.5
6	5304.0
7	5883.5
8	6463.0
9	7114.0
10	7765.0
11	8124.0
12	8483.0
13	8299.0
14	8115.0
15	7659.5
16	7204.0
17	6357.0
18	5510.0
19	5628.0
20	5746.0
21	6499.5
22	7253.0
23	7389.5
24	7526.0
25	9406.0
26	13395.0
27	15504.0
28	16813.5
29	18123.0
30	20501.0
31	22879.0
32	30164.0
33	37449.0
34	39761.0
35	42073.0
36	44041.5
37	46010.0
38	63361.0
39	80712.0
40	112982.5
41	145253.0
42	178737.5
43	212222.0
44	218056.0
45	223890.0
46	215107.5
47	206325.0
48	187499.5
49	168674.0
50	161934.0
51	155194.0
52	143707.0
53	132220.0
54	116385.5
55	100551.0
56	89910.5
57	79270.0
58	70666.0
59	62062.0
60	56731.0
61	51400.0
62	43273.0
63	35146.0
64	30206.5
65	25267.0
66	20823.0
67	16379.0
68	13168.0
69	9957.0
70	8318.5
71	6680.0
72	5181.0
73	3682.0
74	3234.0
75	1873.5
76	961.0
77	735.5
78	510.0
79	390.5
80	271.0
81	198.0
82	125.0
83	95.5
84	66.0
85	48.0
86	30.0
87	24.5
88	19.0
89	10.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	2.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1997773.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.019419243711226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.86286257623249	13.762389594315074
2	8.342327774818232	2.8396314773617615
3	2.4896733005186125	1.2711838104420154
4	1.1338726761595963	0.7719141777819591
5	0.6782026630674142	0.5771307727472874
6	0.4672621944436201	0.47715187103834916
7	0.3202701210649157	0.38155680231465655
8	0.25103586611197487	0.3417987720454288
9	0.20019609042524755	0.30664990745092896
>10	2.1503761864413344	8.754261706617374
>50	1.1430322383318197	14.507926999652772
>100	1.9498603647395556	54.11076419177056
>500	0.00983573708901939	1.0777421921235264
>1k	5.961052781223873E-4	0.12590398761416702
>5k	5.961052781223873E-4	0.6939937367241817
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7698	0.38532906391266675	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5983	0.29948347484924465	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0011147412643979E-4	0.0	0.0	0.03619029789670798	0.0
2	1.0011147412643979E-4	0.0	0.0	0.13505037859656727	0.0
3	1.0011147412643979E-4	0.0	0.0	0.20017289251581635	0.0
4	1.0011147412643979E-4	0.0	0.0	0.34673609063692423	0.0
5	1.0011147412643979E-4	0.0	0.0	0.6210915854804324	0.0
6	1.0011147412643979E-4	0.0	0.0	0.950358223882293	0.0
7	1.0011147412643979E-4	0.0	0.0	1.1652975588317591	0.0
8	1.0011147412643979E-4	0.0	0.0	1.7713223674561624	0.0
9	1.0011147412643979E-4	0.0	0.0	2.063747983379493	0.0
10	1.0011147412643979E-4	0.0	0.0	2.5663075834942206	0.0
11	1.0011147412643979E-4	0.0	0.0	2.9156465724584324	0.0
12	1.0011147412643979E-4	0.0	0.0	3.2561256959624543	0.0
13	1.0011147412643979E-4	0.0	0.0	3.3800136451939236	0.0
14	1.0011147412643979E-4	0.0	0.0	3.417905838150781	0.0
15	1.0011147412643979E-4	0.0	0.0	3.4859315848196966	0.0
16	1.501672111896597E-4	0.0	0.0	3.6311933337771607	0.0
17	2.0022294825287957E-4	0.0	0.0	3.8061881905501775	0.0
18	2.0022294825287957E-4	0.0	0.0	4.114531530859612	0.0
19	2.0022294825287957E-4	0.0	0.0	4.220199191800069	0.0
20	2.0022294825287957E-4	0.0	0.0	4.3453385344581195	0.0
21	2.0022294825287957E-4	0.0	0.0	4.494504630906515	0.0
22	2.0022294825287957E-4	0.0	0.0	4.635511642213605	0.0
23	2.0022294825287957E-4	0.0	0.0	4.804750089224351	0.0
24	2.0022294825287957E-4	0.0	0.0	4.926185307339723	0.0
25	2.0022294825287957E-4	0.0	0.0	5.028949735530513	0.0
26	2.0022294825287957E-4	0.0	0.0	5.131213606350672	0.0
27	2.0022294825287957E-4	0.0	0.0	5.224217165814134	0.0
28	2.0022294825287957E-4	0.0	0.0	5.323627859621689	0.0
29	2.0022294825287957E-4	0.0	0.0	5.429395632036273	0.0
30	2.0022294825287957E-4	0.0	0.0	5.591375997172852	0.0
31	2.0022294825287957E-4	0.0	0.0	5.746148336172328	0.0
32	2.0022294825287957E-4	0.0	0.0	5.867883888710079	0.0
33	2.0022294825287957E-4	0.0	0.0	5.984413644593254	0.0
34	2.0022294825287957E-4	0.0	0.0	6.112656442949224	0.0
35	2.0022294825287957E-4	0.0	0.0	6.296360997971241	0.0
36	2.0022294825287957E-4	0.0	0.0	6.4357662256923085	0.0
37	2.0022294825287957E-4	0.0	0.0	6.577574128792411	0.0
38	2.0022294825287957E-4	0.0	0.0	6.702313025553955	0.0
39	2.0022294825287957E-4	0.0	0.0	6.842268866382717	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAG	40	6.8193913E-9	45.000004	1
CGAACAA	20	7.034538E-4	45.000004	38
CGTATTA	20	7.034538E-4	45.000004	13
GCACGTC	30	2.166309E-6	45.000004	9
ATTCGAC	40	6.8193913E-9	45.000004	40
CGCGACA	30	2.166309E-6	45.000004	28
GCGTTCC	20	7.034538E-4	45.000004	45
TCGCTAA	20	7.034538E-4	45.000004	15
AATCTCG	60	0.0	45.000004	18
ACGTCGT	20	7.034538E-4	45.000004	37
ACGTCGA	20	7.034538E-4	45.000004	20
CTATTCG	20	7.034538E-4	45.000004	1
CGTAAGT	20	7.034538E-4	45.000004	32
ACCGTTG	20	7.034538E-4	45.000004	27
ACCCGGT	20	7.034538E-4	45.000004	20
TCGAACA	20	7.034538E-4	45.000004	34
ATCGATC	20	7.034538E-4	45.000004	33
ATCGAAC	20	7.034538E-4	45.000004	33
TGCGTAT	20	7.034538E-4	45.000004	13
CTTAACG	100	0.0	45.0	1
>>END_MODULE