FastQCFastQC Report
Thu 26 May 2016
SRR1546848_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546848_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2288865
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91410.3993682458336337No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC42710.1865990348928399No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24950.10900599205282968No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGT207.034753E-445.00000419
TCACGAC3600.042.50000425
TGTTACG750.042.0000041
CGTTTTT66950.041.6392821
ATGTACG1250.041.41
GCGTACA556.184564E-1140.90909218
TACGTAG1450.040.3448261
CGTACAC451.9297659E-840.00000419
ACGGTCT4000.039.93749630
TCGTTAG850.039.7058831
CGGTCTA4050.039.44444331
CGCCGTT403.4602272E-739.37500426
TCGTTCA2250.039.016
TAACGCC1450.038.79310212
GGCGATA8150.038.6503078
ATTTACG356.2510044E-638.5714261
GTATCAC700.038.5714269
TATAGCG1400.038.5714261
TAGCATA5250.038.14285730
AGGGCGA18800.038.058516
Produced by FastQC (version 0.11.5)