##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546848_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2288865
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.937598766200715	33.0	31.0	34.0	30.0	34.0
2	32.322322198993824	33.0	31.0	34.0	31.0	34.0
3	32.392174724153676	34.0	31.0	34.0	30.0	34.0
4	35.99231365764254	37.0	35.0	37.0	35.0	37.0
5	35.94317008648391	37.0	35.0	37.0	35.0	37.0
6	35.99549602095362	37.0	35.0	37.0	35.0	37.0
7	36.230811340992155	37.0	35.0	37.0	35.0	37.0
8	36.10352117752685	37.0	35.0	37.0	35.0	37.0
9	37.920502519807854	39.0	39.0	39.0	35.0	39.0
10	37.63980925043635	39.0	37.0	39.0	35.0	39.0
11	37.4045808730528	39.0	37.0	39.0	35.0	39.0
12	36.678179359638946	39.0	35.0	39.0	33.0	39.0
13	36.46842518016572	39.0	35.0	39.0	33.0	39.0
14	37.53549335587726	40.0	35.0	41.0	33.0	41.0
15	37.68865223593353	40.0	35.0	41.0	33.0	41.0
16	37.78585106592132	40.0	35.0	41.0	33.0	41.0
17	37.736575114740276	40.0	35.0	41.0	34.0	41.0
18	37.68709207401922	39.0	35.0	41.0	33.0	41.0
19	37.64239437450439	39.0	35.0	41.0	34.0	41.0
20	37.5296323723767	39.0	35.0	41.0	34.0	41.0
21	37.38723559493461	39.0	35.0	41.0	33.0	41.0
22	37.3369049725519	39.0	35.0	41.0	33.0	41.0
23	37.26660287959316	39.0	35.0	41.0	33.0	41.0
24	37.26223128056919	39.0	35.0	41.0	33.0	41.0
25	37.14798819502242	39.0	35.0	41.0	33.0	41.0
26	37.11635767072326	39.0	35.0	41.0	33.0	41.0
27	37.04943236058046	39.0	35.0	41.0	33.0	41.0
28	37.001545307390344	39.0	35.0	41.0	33.0	41.0
29	36.91169946676628	38.0	35.0	41.0	33.0	41.0
30	36.882207993918385	38.0	35.0	41.0	32.0	41.0
31	36.71161864067999	38.0	35.0	41.0	32.0	41.0
32	36.59072466047583	38.0	35.0	41.0	32.0	41.0
33	36.49673134938059	38.0	35.0	41.0	31.0	41.0
34	36.35670998507994	38.0	35.0	41.0	31.0	41.0
35	36.258990372957776	38.0	35.0	41.0	31.0	41.0
36	36.159849969307935	38.0	35.0	41.0	31.0	41.0
37	35.8953673545622	38.0	35.0	41.0	30.0	41.0
38	35.97662116376458	38.0	35.0	41.0	30.0	41.0
39	35.867868572414714	38.0	35.0	41.0	30.0	41.0
40	35.72935275780791	38.0	35.0	40.0	30.0	41.0
41	35.67465141019676	37.0	35.0	40.0	30.0	41.0
42	35.67706483344365	37.0	35.0	40.0	30.0	41.0
43	35.56685955702936	37.0	35.0	40.0	30.0	41.0
44	35.44004735971759	37.0	35.0	40.0	29.0	41.0
45	35.392831818390334	37.0	35.0	40.0	29.0	41.0
46	35.22518671918178	36.0	35.0	40.0	29.0	41.0
47	35.201363994818394	36.0	35.0	40.0	29.0	41.0
48	35.07553263298622	36.0	34.0	40.0	29.0	41.0
49	35.05761501879753	36.0	34.0	40.0	28.0	41.0
50	34.93345435401389	36.0	34.0	40.0	28.0	41.0
51	34.38114218182374	35.0	34.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	7.0
12	17.0
13	17.0
14	26.0
15	57.0
16	133.0
17	355.0
18	732.0
19	1436.0
20	2518.0
21	3986.0
22	6079.0
23	8804.0
24	12661.0
25	18518.0
26	25504.0
27	30788.0
28	32904.0
29	35752.0
30	40881.0
31	49557.0
32	63077.0
33	86480.0
34	173379.0
35	365943.0
36	142718.0
37	193311.0
38	312071.0
39	680886.0
40	264.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.830232451455196	22.92039941193561	29.07174516627237	13.177622970336827
2	31.531698025003656	25.11891264884561	29.923171528246534	13.426217797904203
3	29.984511974275456	24.502537283762912	31.333259060713498	14.17969168124813
4	27.249444593717847	26.329468972613064	30.607702944472482	15.813383489196612
5	23.631712661078744	31.807118375264597	28.851068105807897	15.710100857848758
6	21.81679566073141	39.918300118180845	26.88446020189046	11.380444019197288
7	85.7257636426788	4.935109759640696	6.995301164550989	2.3438254331295205
8	86.66583656091557	3.966332658326288	6.855100672167209	2.5127301085909393
9	82.13843979439591	5.315778781186308	8.538817274063783	4.006964150353996
10	49.697513833275444	23.17860599030524	14.168507098496416	12.9553730779229
11	43.52764361375616	20.821193036723443	20.59335085293366	15.057812496586736
12	37.6369073754896	21.212653433033406	25.434964491134256	15.715474700342746
13	24.429706426547657	33.210346612840866	25.673379600806513	16.686567359804968
14	18.498994042898993	36.297990488735685	28.45986984815618	16.743145620209145
15	17.00869208100958	23.049284252238557	42.79789327898325	17.144130387768612
16	20.038228554327144	19.087626399984273	40.81372208496351	20.060422960725074
17	20.65857968905986	19.39175093332285	27.91737389492172	32.032295482695574
18	24.653485461134668	22.824238214136702	30.819947878096787	21.702328446631846
19	29.008089162095622	24.672708962739172	25.07378110985139	21.245420765313813
20	30.929041249702365	23.638615645745816	23.784757947716447	21.647585156835376
21	26.059903052386225	25.663724160227886	27.126457873225373	21.14991491416051
22	24.66344673014791	23.23029099575554	26.276779102306165	25.829483171790386
23	22.315645527368368	27.757207174735075	25.356410273214014	24.57073702468254
24	22.692207709934838	24.089581517477	33.02650003385958	20.19171073872858
25	20.968209134221546	24.831958197621965	31.35003593484107	22.84979673331542
26	19.91956712169569	29.226188525754033	27.204050916065388	23.650193436484894
27	20.481330266311033	28.25658131868852	28.85084965692603	22.41123875807442
28	18.701889364379287	26.926882974749493	34.73787226420082	19.633355396670403
29	19.38515377709039	24.9145318749686	33.782507924233194	21.91780642370782
30	21.707046942480225	26.130374661677298	31.06976601940263	21.092812376439852
31	29.057065401410743	24.41568200833164	24.77367603593921	21.753576554318407
32	29.41165162646115	25.780812760909882	25.375677464594897	19.43185814803407
33	27.546928281047595	24.770442992487542	25.581150482881256	22.10147824358361
34	22.10392487106055	25.901877131241903	28.951467211915077	23.042730785782474
35	22.58062402107595	24.96726543505187	29.409336068313337	23.042774475558847
36	28.70378986965155	24.434162783737793	26.376566551544105	20.48548079506655
37	23.748932331089865	28.26392120111933	26.858683233829865	21.128463233960936
38	23.552459406736524	30.459245084354038	23.49339082907904	22.494904679830398
39	23.164100984549112	27.912655399073337	25.554456029516814	23.368787586860737
40	24.955731334089165	23.96283747621638	26.15851087766207	24.92292031203238
41	20.570763238548366	23.81354951034683	27.15481253809202	28.460874713012778
42	23.60833863071872	25.88811485168413	24.650733005223113	25.852813512374034
43	23.127532641724173	25.470091071338853	25.850891162213586	25.55148512472339
44	22.53199729997182	27.7874404999858	27.545573897980002	22.134988302062375
45	21.30012036533391	30.840088865005143	24.304142009249123	23.55564876041182
46	23.668281003903683	28.70252286613671	26.426503965939453	21.20269216402016
47	23.773049087648246	25.91148888204416	27.181419611903717	23.13404241840388
48	24.129164454871738	24.055416112352628	28.527457932206573	23.28796150056906
49	23.260262182348022	22.903709917360786	30.336127294532446	23.49990060575875
50	21.49820981141308	28.36628634716333	27.17761860135919	22.9578852400644
51	20.987694774484297	29.911593737507452	25.26129763004808	23.839413857960167
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1299.0
1	2466.0
2	3633.0
3	10401.5
4	17170.0
5	12300.5
6	7431.0
7	7720.5
8	8010.0
9	8353.5
10	8697.0
11	8606.0
12	8515.0
13	8358.5
14	8202.0
15	7846.5
16	7491.0
17	6889.0
18	6287.0
19	6359.5
20	6432.0
21	6476.5
22	6521.0
23	7127.5
24	7734.0
25	8371.5
26	10119.5
27	11230.0
28	15768.5
29	20307.0
30	23754.0
31	27201.0
32	30505.0
33	33809.0
34	38171.5
35	42534.0
36	46202.0
37	49870.0
38	60375.0
39	70880.0
40	97603.5
41	124327.0
42	150288.5
43	176250.0
44	179655.5
45	183061.0
46	183778.0
47	184495.0
48	185289.5
49	186084.0
50	184783.0
51	183482.0
52	179519.5
53	175557.0
54	158817.0
55	142077.0
56	135297.5
57	128518.0
58	119326.5
59	110135.0
60	104999.0
61	99863.0
62	86200.5
63	72538.0
64	64613.5
65	56689.0
66	46575.5
67	36462.0
68	30606.5
69	24751.0
70	20665.0
71	16579.0
72	13889.5
73	11200.0
74	9582.0
75	5500.5
76	3037.0
77	2387.0
78	1737.0
79	1282.0
80	827.0
81	623.0
82	419.0
83	328.0
84	237.0
85	233.5
86	230.0
87	135.5
88	41.0
89	33.0
90	25.0
91	16.0
92	7.0
93	6.5
94	6.0
95	4.5
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2288865.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.255337811239105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.50256972787889	15.693595125943782
2	6.915829995439557	2.663332856145778
3	2.104711188374438	1.2158077478173288
4	1.0490942566885701	0.8080265723347684
5	0.6839098341557414	0.6584457444548656
6	0.4470762635575324	0.5165162689312112
7	0.34360114896389543	0.4631309336940787
8	0.27100083485756	0.41745700978481115
9	0.2539444737071845	0.44008079638762476
>10	3.3244995900075622	16.160070681878683
>50	1.8741373223575009	26.565017613284354
>100	1.2215554432633746	32.44385758949435
>500	0.005533659941614234	0.7446687250286578
>1k	0.002305691642339264	0.8041605327874606
>5k	2.305691642339264E-4	0.4058318020322519
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9141	0.3993682458336337	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4271	0.1865990348928399	No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	2495	0.10900599205282968	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.310693291216389E-4	0.0	0.0	0.028835252406760555	0.0
2	1.310693291216389E-4	0.0	0.0	0.10253990514949549	0.0
3	1.310693291216389E-4	0.0	0.0	0.14784620324920866	0.0
4	1.310693291216389E-4	0.0	0.0	0.2277984940134084	0.0
5	1.310693291216389E-4	0.0	0.0	0.4175431928051676	0.0
6	1.310693291216389E-4	0.0	0.0	0.6636040133428577	0.0
7	1.310693291216389E-4	0.0	0.0	0.8376640824163941	0.0
8	1.310693291216389E-4	0.0	0.0	1.3007320222031444	0.0
9	1.310693291216389E-4	0.0	0.0	1.5464433245298435	0.0
10	1.310693291216389E-4	0.0	0.0	1.9484766467222838	0.0
11	1.310693291216389E-4	0.0	0.0	2.2356932366041686	0.0
12	1.310693291216389E-4	0.0	0.0	2.510545619772245	0.0
13	1.310693291216389E-4	0.0	0.0	2.6174981923355025	0.0
14	1.310693291216389E-4	0.0	0.0	2.657255888835733	0.0
15	1.310693291216389E-4	0.0	0.0	2.716062327834975	0.0
16	2.1844888186939816E-4	0.0	0.0	2.8432869566357124	0.0
17	2.1844888186939816E-4	0.0	0.0	2.9849292116398303	0.0
18	2.1844888186939816E-4	0.0	0.0	3.211460702138396	0.0
19	2.1844888186939816E-4	0.0	0.0	3.304825754249377	0.0
20	2.1844888186939816E-4	0.0	0.0	3.416147304450022	0.0
21	2.621386582432778E-4	0.0	0.0	3.546168078938688	0.0
22	2.621386582432778E-4	0.0	0.0	3.6839656336219044	0.0
23	3.0582843461715744E-4	0.0	0.0	3.83875851131456	0.0
24	3.0582843461715744E-4	0.0	0.0	3.9560655608784265	0.0
25	3.0582843461715744E-4	0.0	0.0	4.047027675288844	0.0
26	3.0582843461715744E-4	0.0	0.0	4.141310212703677	0.0
27	3.0582843461715744E-4	0.0	0.0	4.243238460983937	0.0
28	3.0582843461715744E-4	0.0	0.0	4.346783230990032	0.0
29	3.0582843461715744E-4	0.0	0.0	4.462124240617074	0.0
30	3.0582843461715744E-4	0.0	0.0	4.616873428533356	0.0
31	3.932079873649167E-4	0.0	0.0	4.746632064363778	0.0
32	3.932079873649167E-4	0.0	0.0	4.870623649712849	0.0
33	3.932079873649167E-4	0.0	0.0	4.996799723880613	0.0
34	3.932079873649167E-4	0.0	0.0	5.12987878271545	0.0
35	3.932079873649167E-4	0.0	0.0	5.290263951783963	0.0
36	3.932079873649167E-4	0.0	0.0	5.430114925956752	0.0
37	4.3689776373879633E-4	0.0	0.0	5.562451258593233	0.0
38	4.3689776373879633E-4	0.0	0.0	5.716108202100168	0.0
39	4.3689776373879633E-4	0.0	0.0	5.921275391951906	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATCGT	20	7.034753E-4	45.000004	19
TCACGAC	360	0.0	42.500004	25
TGTTACG	75	0.0	42.000004	1
CGTTTTT	6695	0.0	41.639282	1
ATGTACG	125	0.0	41.4	1
GCGTACA	55	6.184564E-11	40.909092	18
TACGTAG	145	0.0	40.344826	1
CGTACAC	45	1.9297659E-8	40.000004	19
ACGGTCT	400	0.0	39.937496	30
TCGTTAG	85	0.0	39.705883	1
CGGTCTA	405	0.0	39.444443	31
CGCCGTT	40	3.4602272E-7	39.375004	26
TCGTTCA	225	0.0	39.0	16
TAACGCC	145	0.0	38.793102	12
GGCGATA	815	0.0	38.650307	8
ATTTACG	35	6.2510044E-6	38.571426	1
GTATCAC	70	0.0	38.571426	9
TATAGCG	140	0.0	38.571426	1
TAGCATA	525	0.0	38.142857	30
AGGGCGA	1880	0.0	38.05851	6
>>END_MODULE